Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05030 and PS417_05035 overlap by 4 nucleotides PS417_05035 and PS417_05040 are separated by 187 nucleotides PS417_05040 and PS417_05045 are separated by 8 nucleotides
PS417_05030: PS417_05030 - major facilitator transporter, at 1,118,735 to 1,120,102
_05030
PS417_05035: PS417_05035 - hypothetical protein, at 1,120,099 to 1,120,902
_05035
PS417_05040: PS417_05040 - amino acid dehydrogenase, at 1,121,090 to 1,122,109
_05040
PS417_05045: PS417_05045 - acetamidase, at 1,122,118 to 1,123,425
_05045
Position (kb)
1121
1122
1123 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1120.492 kb on + strand, within PS417_05035 at 1120.492 kb on + strand, within PS417_05035 at 1120.493 kb on - strand, within PS417_05035 at 1120.804 kb on + strand, within PS417_05035 at 1120.805 kb on - strand, within PS417_05035 at 1120.805 kb on - strand, within PS417_05035 at 1120.805 kb on - strand, within PS417_05035 at 1120.981 kb on + strand at 1120.981 kb on + strand at 1120.981 kb on + strand at 1120.982 kb on - strand at 1121.012 kb on + strand at 1121.013 kb on - strand at 1121.049 kb on + strand at 1121.050 kb on - strand at 1121.050 kb on - strand at 1121.051 kb on + strand at 1121.051 kb on + strand at 1121.052 kb on - strand at 1121.052 kb on - strand at 1121.052 kb on - strand at 1121.282 kb on + strand, within PS417_05040 at 1121.282 kb on + strand, within PS417_05040 at 1121.282 kb on + strand, within PS417_05040 at 1121.282 kb on + strand, within PS417_05040 at 1121.411 kb on + strand at 1121.412 kb on - strand, within PS417_05040 at 1121.949 kb on - strand, within PS417_05040 at 1122.110 kb on + strand at 1122.110 kb on + strand at 1122.111 kb on - strand at 1122.111 kb on - strand at 1122.128 kb on + strand at 1122.348 kb on + strand, within PS417_05045 at 1122.368 kb on - strand, within PS417_05045 at 1122.533 kb on - strand, within PS417_05045 at 1122.593 kb on + strand, within PS417_05045 at 1122.594 kb on - strand, within PS417_05045 at 1122.734 kb on + strand, within PS417_05045 at 1122.983 kb on + strand, within PS417_05045 at 1122.984 kb on - strand, within PS417_05045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,120,492 + PS417_05035 0.49 +0.1 1,120,492 + PS417_05035 0.49 +1.0 1,120,493 - PS417_05035 0.49 +0.0 1,120,804 + PS417_05035 0.88 +0.3 1,120,805 - PS417_05035 0.88 +0.5 1,120,805 - PS417_05035 0.88 +0.2 1,120,805 - PS417_05035 0.88 -0.8 1,120,981 + -1.1 1,120,981 + -1.5 1,120,981 + -0.0 1,120,982 - +0.5 1,121,012 + +0.1 1,121,013 - +0.6 1,121,049 + -0.3 1,121,050 - -0.4 1,121,050 - -0.1 1,121,051 + -0.3 1,121,051 + -1.0 1,121,052 - +0.6 1,121,052 - -0.7 1,121,052 - -0.4 1,121,282 + PS417_05040 0.19 -1.7 1,121,282 + PS417_05040 0.19 -0.9 1,121,282 + PS417_05040 0.19 +0.2 1,121,282 + PS417_05040 0.19 -1.8 1,121,411 + +0.6 1,121,412 - PS417_05040 0.32 -0.6 1,121,949 - PS417_05040 0.84 -0.7 1,122,110 + +0.7 1,122,110 + -0.3 1,122,111 - +0.7 1,122,111 - +1.1 1,122,128 + +1.5 1,122,348 + PS417_05045 0.18 -1.5 1,122,368 - PS417_05045 0.19 +0.2 1,122,533 - PS417_05045 0.32 -1.5 1,122,593 + PS417_05045 0.36 -0.7 1,122,594 - PS417_05045 0.36 -0.6 1,122,734 + PS417_05045 0.47 -1.2 1,122,983 + PS417_05045 0.66 +3.6 1,122,984 - PS417_05045 0.66 +1.6
Or see this region's nucleotide sequence