Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04420 and PS417_04425 are separated by 3 nucleotides PS417_04425 and PS417_04430 are separated by 124 nucleotides
PS417_04420: PS417_04420 - histidinol dehydrogenase, at 982,018 to 983,340
_04420
PS417_04425: PS417_04425 - histidinol-phosphate aminotransferase, at 983,344 to 984,396
_04425
PS417_04430: PS417_04430 - uracil transporter, at 984,521 to 985,795
_04430
Position (kb) 983
984
985 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 982.360 kb on + strand, within PS417_04420 at 982.360 kb on + strand, within PS417_04420 at 982.423 kb on + strand, within PS417_04420 at 982.423 kb on + strand, within PS417_04420 at 982.628 kb on - strand, within PS417_04420 at 982.681 kb on + strand, within PS417_04420 at 982.681 kb on + strand, within PS417_04420 at 982.681 kb on + strand, within PS417_04420 at 982.682 kb on - strand, within PS417_04420 at 982.682 kb on - strand, within PS417_04420 at 982.893 kb on + strand, within PS417_04420 at 982.893 kb on + strand, within PS417_04420 at 982.893 kb on + strand, within PS417_04420 at 982.894 kb on - strand, within PS417_04420 at 982.894 kb on - strand at 982.894 kb on - strand, within PS417_04420 at 983.120 kb on - strand, within PS417_04420 at 983.188 kb on + strand, within PS417_04420 at 983.188 kb on + strand, within PS417_04420 at 983.189 kb on - strand, within PS417_04420 at 983.189 kb on - strand, within PS417_04420 at 983.189 kb on - strand, within PS417_04420 at 983.350 kb on - strand at 983.386 kb on + strand at 983.386 kb on + strand at 983.386 kb on + strand at 983.386 kb on + strand at 983.386 kb on + strand at 983.387 kb on - strand at 983.557 kb on + strand, within PS417_04425 at 983.558 kb on - strand, within PS417_04425 at 983.558 kb on - strand, within PS417_04425 at 983.558 kb on - strand, within PS417_04425 at 983.675 kb on - strand, within PS417_04425 at 983.823 kb on - strand, within PS417_04425 at 983.823 kb on - strand, within PS417_04425 at 983.894 kb on - strand, within PS417_04425 at 983.894 kb on - strand, within PS417_04425 at 983.894 kb on - strand, within PS417_04425 at 983.999 kb on + strand at 984.000 kb on - strand, within PS417_04425 at 984.124 kb on + strand, within PS417_04425 at 984.125 kb on - strand, within PS417_04425 at 984.163 kb on - strand, within PS417_04425 at 984.458 kb on + strand at 984.458 kb on + strand at 984.459 kb on - strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.470 kb on - strand at 984.519 kb on - strand at 984.523 kb on - strand at 984.523 kb on - strand at 984.842 kb on - strand, within PS417_04430 at 984.900 kb on + strand, within PS417_04430 at 984.900 kb on + strand, within PS417_04430 at 984.900 kb on + strand, within PS417_04430 at 984.939 kb on + strand, within PS417_04430 at 984.940 kb on - strand, within PS417_04430 at 984.940 kb on - strand at 985.210 kb on - strand, within PS417_04430 at 985.381 kb on + strand, within PS417_04430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 982,360 + PS417_04420 0.26 -0.7 982,360 + PS417_04420 0.26 +0.4 982,423 + PS417_04420 0.31 -2.7 982,423 + PS417_04420 0.31 -0.3 982,628 - PS417_04420 0.46 -0.9 982,681 + PS417_04420 0.50 -0.5 982,681 + PS417_04420 0.50 -0.3 982,681 + PS417_04420 0.50 -2.1 982,682 - PS417_04420 0.50 +0.1 982,682 - PS417_04420 0.50 -0.7 982,893 + PS417_04420 0.66 -1.8 982,893 + PS417_04420 0.66 -1.6 982,893 + PS417_04420 0.66 -0.4 982,894 - PS417_04420 0.66 -0.3 982,894 - -1.2 982,894 - PS417_04420 0.66 -0.7 983,120 - PS417_04420 0.83 -0.4 983,188 + PS417_04420 0.88 -1.0 983,188 + PS417_04420 0.88 -1.6 983,189 - PS417_04420 0.89 -0.2 983,189 - PS417_04420 0.89 -0.6 983,189 - PS417_04420 0.89 -1.5 983,350 - -1.4 983,386 + -1.5 983,386 + -1.7 983,386 + +0.6 983,386 + +0.5 983,386 + +1.3 983,387 - +0.3 983,557 + PS417_04425 0.20 -0.1 983,558 - PS417_04425 0.20 -0.9 983,558 - PS417_04425 0.20 -0.7 983,558 - PS417_04425 0.20 -0.6 983,675 - PS417_04425 0.31 -1.6 983,823 - PS417_04425 0.45 +0.4 983,823 - PS417_04425 0.45 -0.9 983,894 - PS417_04425 0.52 -0.5 983,894 - PS417_04425 0.52 -1.4 983,894 - PS417_04425 0.52 +2.1 983,999 + -0.4 984,000 - PS417_04425 0.62 -0.7 984,124 + PS417_04425 0.74 +1.6 984,125 - PS417_04425 0.74 +0.2 984,163 - PS417_04425 0.78 +0.2 984,458 + +0.6 984,458 + +0.2 984,459 - -0.6 984,469 + +0.6 984,469 + +0.5 984,469 + +0.4 984,469 + +1.2 984,469 + +0.4 984,469 + -0.1 984,470 - -0.5 984,519 - -0.6 984,523 - +1.2 984,523 - -0.5 984,842 - PS417_04430 0.25 +3.2 984,900 + PS417_04430 0.30 -0.3 984,900 + PS417_04430 0.30 -0.3 984,900 + PS417_04430 0.30 -0.1 984,939 + PS417_04430 0.33 +0.4 984,940 - PS417_04430 0.33 -1.7 984,940 - -0.9 985,210 - PS417_04430 0.54 +0.1 985,381 + PS417_04430 0.67 -1.0
Or see this region's nucleotide sequence