Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02915 and PS417_02920 are separated by 236 nucleotides PS417_02920 and PS417_02925 overlap by 4 nucleotides PS417_02925 and PS417_02930 are separated by 163 nucleotides
PS417_02915: PS417_02915 - precorrin-3B C17-methyltransferase, at 664,507 to 666,144
_02915
PS417_02920: PS417_02920 - hypothetical protein, at 666,381 to 667,160
_02920
PS417_02925: PS417_02925 - membrane protein, at 667,157 to 667,753
_02925
PS417_02930: PS417_02930 - histidine kinase, at 667,917 to 670,688
_02930
Position (kb) 666
667
668 Strain fitness (log2 ratio) -2
-1
0
1
2 at 665.482 kb on + strand, within PS417_02915 at 665.483 kb on - strand, within PS417_02915 at 665.603 kb on + strand, within PS417_02915 at 665.866 kb on + strand, within PS417_02915 at 665.866 kb on + strand, within PS417_02915 at 665.867 kb on - strand at 666.335 kb on + strand at 666.336 kb on - strand at 666.347 kb on - strand at 666.347 kb on - strand at 666.347 kb on - strand at 666.347 kb on - strand at 666.378 kb on - strand at 666.410 kb on + strand at 666.438 kb on + strand at 666.568 kb on - strand, within PS417_02920 at 666.575 kb on + strand, within PS417_02920 at 666.583 kb on - strand, within PS417_02920 at 666.583 kb on - strand, within PS417_02920 at 666.583 kb on - strand, within PS417_02920 at 666.583 kb on - strand, within PS417_02920 at 666.583 kb on - strand, within PS417_02920 at 666.600 kb on + strand, within PS417_02920 at 666.600 kb on + strand, within PS417_02920 at 666.600 kb on + strand, within PS417_02920 at 666.600 kb on + strand, within PS417_02920 at 666.601 kb on - strand, within PS417_02920 at 666.601 kb on - strand, within PS417_02920 at 666.601 kb on - strand, within PS417_02920 at 666.759 kb on - strand, within PS417_02920 at 666.811 kb on + strand, within PS417_02920 at 666.856 kb on + strand, within PS417_02920 at 666.969 kb on + strand, within PS417_02920 at 666.970 kb on - strand, within PS417_02920 at 666.970 kb on - strand, within PS417_02920 at 666.997 kb on + strand, within PS417_02920 at 666.998 kb on - strand, within PS417_02920 at 667.454 kb on + strand, within PS417_02925 at 667.455 kb on - strand, within PS417_02925 at 667.455 kb on - strand, within PS417_02925 at 667.455 kb on - strand, within PS417_02925 at 667.455 kb on - strand, within PS417_02925 at 667.588 kb on - strand, within PS417_02925 at 667.679 kb on - strand, within PS417_02925 at 667.708 kb on - strand at 667.848 kb on + strand at 667.848 kb on + strand at 667.911 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 665,482 + PS417_02915 0.60 -0.5 665,483 - PS417_02915 0.60 -0.6 665,603 + PS417_02915 0.67 -1.0 665,866 + PS417_02915 0.83 -2.1 665,866 + PS417_02915 0.83 -0.1 665,867 - -1.0 666,335 + -0.4 666,336 - -0.1 666,347 - +1.0 666,347 - -0.2 666,347 - +0.1 666,347 - -0.3 666,378 - +1.6 666,410 + -0.1 666,438 + +2.4 666,568 - PS417_02920 0.24 -2.7 666,575 + PS417_02920 0.25 -0.1 666,583 - PS417_02920 0.26 -0.2 666,583 - PS417_02920 0.26 -1.6 666,583 - PS417_02920 0.26 -0.6 666,583 - PS417_02920 0.26 -0.3 666,583 - PS417_02920 0.26 -1.8 666,600 + PS417_02920 0.28 +0.4 666,600 + PS417_02920 0.28 -0.2 666,600 + PS417_02920 0.28 -0.4 666,600 + PS417_02920 0.28 +0.2 666,601 - PS417_02920 0.28 +1.6 666,601 - PS417_02920 0.28 -0.9 666,601 - PS417_02920 0.28 -0.4 666,759 - PS417_02920 0.48 +0.4 666,811 + PS417_02920 0.55 -1.0 666,856 + PS417_02920 0.61 -1.0 666,969 + PS417_02920 0.75 +0.0 666,970 - PS417_02920 0.76 +0.0 666,970 - PS417_02920 0.76 -0.1 666,997 + PS417_02920 0.79 -1.4 666,998 - PS417_02920 0.79 -1.3 667,454 + PS417_02925 0.50 -0.4 667,455 - PS417_02925 0.50 -0.1 667,455 - PS417_02925 0.50 +0.3 667,455 - PS417_02925 0.50 -0.3 667,455 - PS417_02925 0.50 +0.2 667,588 - PS417_02925 0.72 -1.5 667,679 - PS417_02925 0.87 +1.6 667,708 - -0.4 667,848 + -1.4 667,848 + -1.3 667,911 + +0.7
Or see this region's nucleotide sequence