Strain Fitness in Pseudomonas simiae WCS417 around PS417_00270

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00265 and PS417_00270 are separated by 12 nucleotidesPS417_00270 and PS417_00275 are separated by 77 nucleotidesPS417_00275 and PS417_00280 are separated by 223 nucleotides PS417_00265: PS417_00265 - GMC family oxidoreductase, at 50,691 to 52,475 _00265 PS417_00270: PS417_00270 - alcohol dehydrogenase, at 52,488 to 53,750 _00270 PS417_00275: PS417_00275 - hypothetical protein, at 53,828 to 54,085 _00275 PS417_00280: PS417_00280 - radical SAM protein, at 54,309 to 55,367 _00280 Position (kb) 52 53 54Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 51.579 kb on + strand, within PS417_00265at 51.579 kb on + strand, within PS417_00265at 51.580 kb on - strand, within PS417_00265at 51.580 kb on - strand, within PS417_00265at 51.580 kb on - strand, within PS417_00265at 51.580 kb on - strand, within PS417_00265at 51.736 kb on - strand, within PS417_00265at 51.984 kb on + strand, within PS417_00265at 51.987 kb on + strand, within PS417_00265at 51.987 kb on + strand, within PS417_00265at 51.988 kb on - strand, within PS417_00265at 51.988 kb on - strand, within PS417_00265at 52.040 kb on + strand, within PS417_00265at 52.044 kb on + strand, within PS417_00265at 52.045 kb on - strand, within PS417_00265at 52.045 kb on - strand, within PS417_00265at 52.240 kb on - strand, within PS417_00265at 52.377 kb on + strandat 52.410 kb on + strandat 52.473 kb on + strandat 52.674 kb on + strand, within PS417_00270at 52.675 kb on - strand, within PS417_00270at 52.717 kb on - strand, within PS417_00270at 52.717 kb on - strand, within PS417_00270at 52.770 kb on - strand, within PS417_00270at 52.772 kb on + strand, within PS417_00270at 52.772 kb on + strand, within PS417_00270at 52.772 kb on + strand, within PS417_00270at 52.772 kb on + strand, within PS417_00270at 52.773 kb on - strand, within PS417_00270at 52.773 kb on - strand, within PS417_00270at 52.803 kb on + strand, within PS417_00270at 52.803 kb on + strand, within PS417_00270at 52.839 kb on + strand, within PS417_00270at 52.840 kb on - strand, within PS417_00270at 52.840 kb on - strand, within PS417_00270at 52.846 kb on - strand, within PS417_00270at 52.846 kb on - strand, within PS417_00270at 53.052 kb on - strand, within PS417_00270at 53.246 kb on - strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.286 kb on + strand, within PS417_00270at 53.287 kb on - strand, within PS417_00270at 53.287 kb on - strand, within PS417_00270at 53.287 kb on - strand, within PS417_00270at 53.287 kb on - strand, within PS417_00270at 53.287 kb on - strand, within PS417_00270at 53.287 kb on - strand, within PS417_00270at 53.370 kb on + strand, within PS417_00270at 53.371 kb on - strand, within PS417_00270at 53.371 kb on - strand, within PS417_00270at 53.371 kb on - strand, within PS417_00270at 53.433 kb on + strand, within PS417_00270at 53.433 kb on + strand, within PS417_00270at 53.434 kb on - strand, within PS417_00270at 53.434 kb on - strand, within PS417_00270at 53.434 kb on - strand, within PS417_00270at 53.550 kb on - strand, within PS417_00270at 53.568 kb on - strand, within PS417_00270at 53.684 kb on + strandat 53.684 kb on + strandat 53.722 kb on + strandat 53.722 kb on + strandat 53.778 kb on - strandat 53.793 kb on - strandat 53.820 kb on + strandat 53.949 kb on + strand, within PS417_00275at 53.949 kb on + strand, within PS417_00275at 53.949 kb on + strand, within PS417_00275at 53.949 kb on + strand, within PS417_00275at 53.979 kb on + strand, within PS417_00275at 53.979 kb on + strand, within PS417_00275at 53.980 kb on - strand, within PS417_00275at 53.980 kb on - strand, within PS417_00275at 54.085 kb on + strandat 54.085 kb on + strandat 54.085 kb on + strandat 54.086 kb on - strandat 54.087 kb on + strandat 54.110 kb on + strandat 54.110 kb on + strandat 54.129 kb on - strandat 54.191 kb on - strandat 54.194 kb on + strandat 54.195 kb on - strandat 54.195 kb on - strandat 54.197 kb on + strandat 54.198 kb on - strandat 54.282 kb on - strandat 54.302 kb on + strandat 54.303 kb on - strandat 54.543 kb on + strand, within PS417_00280at 54.543 kb on + strandat 54.543 kb on + strand, within PS417_00280at 54.544 kb on - strand, within PS417_00280at 54.544 kb on - strand, within PS417_00280at 54.544 kb on - strand, within PS417_00280at 54.549 kb on + strand, within PS417_00280at 54.550 kb on - strand, within PS417_00280at 54.550 kb on - strand, within PS417_00280at 54.571 kb on - strand, within PS417_00280at 54.579 kb on - strand, within PS417_00280at 54.579 kb on - strand, within PS417_00280at 54.666 kb on + strand, within PS417_00280at 54.666 kb on + strand, within PS417_00280at 54.666 kb on + strand, within PS417_00280at 54.667 kb on - strand, within PS417_00280at 54.667 kb on - strand, within PS417_00280at 54.730 kb on - strand, within PS417_00280

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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51,579 + PS417_00265 0.50 -0.3
51,579 + PS417_00265 0.50 -2.3
51,580 - PS417_00265 0.50 -0.5
51,580 - PS417_00265 0.50 +0.6
51,580 - PS417_00265 0.50 -1.0
51,580 - PS417_00265 0.50 -1.5
51,736 - PS417_00265 0.59 -0.8
51,984 + PS417_00265 0.72 +0.5
51,987 + PS417_00265 0.73 -1.2
51,987 + PS417_00265 0.73 +0.0
51,988 - PS417_00265 0.73 -1.4
51,988 - PS417_00265 0.73 +1.4
52,040 + PS417_00265 0.76 -0.2
52,044 + PS417_00265 0.76 +0.5
52,045 - PS417_00265 0.76 -0.1
52,045 - PS417_00265 0.76 +0.1
52,240 - PS417_00265 0.87 -0.4
52,377 + -1.0
52,410 + +0.7
52,473 + -0.5
52,674 + PS417_00270 0.15 -0.1
52,675 - PS417_00270 0.15 -0.4
52,717 - PS417_00270 0.18 -0.8
52,717 - PS417_00270 0.18 -1.0
52,770 - PS417_00270 0.22 +0.7
52,772 + PS417_00270 0.22 +0.4
52,772 + PS417_00270 0.22 -0.3
52,772 + PS417_00270 0.22 -0.7
52,772 + PS417_00270 0.22 -0.4
52,773 - PS417_00270 0.23 +0.3
52,773 - PS417_00270 0.23 -1.0
52,803 + PS417_00270 0.25 -0.9
52,803 + PS417_00270 0.25 -0.7
52,839 + PS417_00270 0.28 +1.7
52,840 - PS417_00270 0.28 +1.6
52,840 - PS417_00270 0.28 -1.3
52,846 - PS417_00270 0.28 -0.0
52,846 - PS417_00270 0.28 -0.3
53,052 - PS417_00270 0.45 -0.1
53,246 - PS417_00270 0.60 -0.7
53,286 + PS417_00270 0.63 -0.5
53,286 + PS417_00270 0.63 -0.3
53,286 + PS417_00270 0.63 +1.1
53,286 + PS417_00270 0.63 -0.9
53,286 + PS417_00270 0.63 -0.4
53,286 + PS417_00270 0.63 +0.0
53,286 + PS417_00270 0.63 -0.3
53,286 + PS417_00270 0.63 -0.5
53,286 + PS417_00270 0.63 +0.1
53,286 + PS417_00270 0.63 -2.1
53,287 - PS417_00270 0.63 +0.1
53,287 - PS417_00270 0.63 +0.6
53,287 - PS417_00270 0.63 +3.7
53,287 - PS417_00270 0.63 -0.7
53,287 - PS417_00270 0.63 -2.3
53,287 - PS417_00270 0.63 -0.5
53,370 + PS417_00270 0.70 -0.3
53,371 - PS417_00270 0.70 +0.1
53,371 - PS417_00270 0.70 -0.4
53,371 - PS417_00270 0.70 -0.8
53,433 + PS417_00270 0.75 -1.4
53,433 + PS417_00270 0.75 -1.2
53,434 - PS417_00270 0.75 -1.9
53,434 - PS417_00270 0.75 -0.8
53,434 - PS417_00270 0.75 -0.7
53,550 - PS417_00270 0.84 -1.5
53,568 - PS417_00270 0.86 -1.1
53,684 + -0.4
53,684 + +0.3
53,722 + -0.6
53,722 + -0.4
53,778 - +0.3
53,793 - -0.1
53,820 + +1.1
53,949 + PS417_00275 0.47 +0.1
53,949 + PS417_00275 0.47 +1.4
53,949 + PS417_00275 0.47 -0.5
53,949 + PS417_00275 0.47 +0.5
53,979 + PS417_00275 0.59 -0.1
53,979 + PS417_00275 0.59 -0.0
53,980 - PS417_00275 0.59 -0.4
53,980 - PS417_00275 0.59 -0.6
54,085 + -1.7
54,085 + +0.2
54,085 + -0.5
54,086 - -1.0
54,087 + -2.0
54,110 + +0.9
54,110 + -0.2
54,129 - -1.1
54,191 - -1.2
54,194 + -0.1
54,195 - +1.2
54,195 - +0.1
54,197 + -0.3
54,198 - +1.0
54,282 - -0.2
54,302 + -0.3
54,303 - -0.6
54,543 + PS417_00280 0.22 -1.1
54,543 + +4.2
54,543 + PS417_00280 0.22 +2.2
54,544 - PS417_00280 0.22 -0.4
54,544 - PS417_00280 0.22 -0.0
54,544 - PS417_00280 0.22 +0.0
54,549 + PS417_00280 0.23 +0.1
54,550 - PS417_00280 0.23 +0.4
54,550 - PS417_00280 0.23 -1.0
54,571 - PS417_00280 0.25 +1.6
54,579 - PS417_00280 0.25 -0.4
54,579 - PS417_00280 0.25 +0.8
54,666 + PS417_00280 0.34 -0.8
54,666 + PS417_00280 0.34 -0.1
54,666 + PS417_00280 0.34 -1.8
54,667 - PS417_00280 0.34 -0.1
54,667 - PS417_00280 0.34 +0.6
54,730 - PS417_00280 0.40 -0.1

Or see this region's nucleotide sequence