Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25645 and PS417_25650 are separated by 3 nucleotides PS417_25650 and PS417_25655 are separated by 77 nucleotides PS417_25655 and PS417_25660 are separated by 165 nucleotides
PS417_25645: PS417_25645 - peptidase M23, at 5,539,791 to 5,541,209
_25645
PS417_25650: PS417_25650 - anhydro-N-acetylmuramic acid kinase, at 5,541,213 to 5,542,304
_25650
PS417_25655: PS417_25655 - iron--sulfur cluster insertion protein ErpA, at 5,542,382 to 5,542,732
_25655
PS417_25660: PS417_25660 - N-acetyl-gamma-glutamyl-phosphate reductase, at 5,542,898 to 5,543,932
_25660
Position (kb)
5541
5542
5543 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5540.415 kb on - strand, within PS417_25645 at 5540.641 kb on - strand, within PS417_25645 at 5540.667 kb on + strand, within PS417_25645 at 5540.670 kb on + strand, within PS417_25645 at 5540.687 kb on + strand, within PS417_25645 at 5540.687 kb on + strand, within PS417_25645 at 5540.732 kb on + strand, within PS417_25645 at 5540.732 kb on + strand, within PS417_25645 at 5540.733 kb on - strand, within PS417_25645 at 5540.916 kb on + strand, within PS417_25645 at 5541.042 kb on + strand, within PS417_25645 at 5541.222 kb on + strand at 5541.222 kb on + strand at 5541.312 kb on + strand at 5541.313 kb on - strand at 5541.313 kb on - strand at 5541.329 kb on + strand, within PS417_25650 at 5541.330 kb on - strand, within PS417_25650 at 5541.334 kb on + strand, within PS417_25650 at 5541.576 kb on - strand, within PS417_25650 at 5541.576 kb on - strand, within PS417_25650 at 5541.803 kb on - strand, within PS417_25650 at 5542.369 kb on + strand at 5542.769 kb on - strand at 5542.769 kb on - strand at 5542.823 kb on + strand at 5542.824 kb on - strand at 5542.824 kb on - strand at 5542.836 kb on - strand at 5542.900 kb on - strand at 5542.900 kb on - strand at 5542.900 kb on - strand at 5543.238 kb on - strand, within PS417_25660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,540,415 - PS417_25645 0.44 -1.7 5,540,641 - PS417_25645 0.60 -1.4 5,540,667 + PS417_25645 0.62 -2.9 5,540,670 + PS417_25645 0.62 -1.2 5,540,687 + PS417_25645 0.63 -2.5 5,540,687 + PS417_25645 0.63 -2.4 5,540,732 + PS417_25645 0.66 -1.5 5,540,732 + PS417_25645 0.66 -1.5 5,540,733 - PS417_25645 0.66 -1.2 5,540,916 + PS417_25645 0.79 -1.4 5,541,042 + PS417_25645 0.88 -4.4 5,541,222 + +0.2 5,541,222 + -0.9 5,541,312 + -3.0 5,541,313 - -3.2 5,541,313 - -1.0 5,541,329 + PS417_25650 0.11 -2.5 5,541,330 - PS417_25650 0.11 -2.6 5,541,334 + PS417_25650 0.11 -2.2 5,541,576 - PS417_25650 0.33 -1.3 5,541,576 - PS417_25650 0.33 -1.0 5,541,803 - PS417_25650 0.54 -3.0 5,542,369 + -1.2 5,542,769 - +2.4 5,542,769 - +0.1 5,542,823 + -0.6 5,542,824 - +0.8 5,542,824 - -0.7 5,542,836 - +0.9 5,542,900 - -1.2 5,542,900 - -1.6 5,542,900 - +0.6 5,543,238 - PS417_25660 0.33 -1.5
Or see this region's nucleotide sequence