Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02415 and PS417_02420 are separated by 123 nucleotides PS417_02420 and PS417_02425 are separated by 15 nucleotides PS417_02425 and PS417_02430 are separated by 57 nucleotides
PS417_02415: PS417_02415 - phosphoserine phosphatase, at 553,413 to 554,627
_02415
PS417_02420: PS417_02420 - phosphatidylserine decarboxylase, at 554,751 to 555,611
_02420
PS417_02425: PS417_02425 - thiosulfate sulfurtransferase, at 555,627 to 556,442
_02425
PS417_02430: PS417_02430 - histidine kinase, at 556,500 to 558,035
_02430
Position (kb) 555
556
557 Strain fitness (log2 ratio) -1
0
1
2 at 554.707 kb on - strand at 554.759 kb on + strand at 554.760 kb on - strand at 554.760 kb on - strand at 555.622 kb on - strand at 555.683 kb on - strand at 555.683 kb on - strand at 555.724 kb on + strand, within PS417_02425 at 555.725 kb on - strand, within PS417_02425 at 555.986 kb on - strand, within PS417_02425 at 556.115 kb on - strand, within PS417_02425 at 556.193 kb on - strand, within PS417_02425 at 556.193 kb on - strand, within PS417_02425 at 556.193 kb on - strand, within PS417_02425 at 556.557 kb on - strand at 556.775 kb on + strand, within PS417_02430 at 556.776 kb on - strand, within PS417_02430 at 556.778 kb on + strand, within PS417_02430 at 556.779 kb on - strand, within PS417_02430 at 556.779 kb on - strand, within PS417_02430 at 556.973 kb on + strand, within PS417_02430 at 557.049 kb on + strand, within PS417_02430 at 557.049 kb on + strand, within PS417_02430 at 557.049 kb on + strand, within PS417_02430 at 557.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 554,707 - +1.9 554,759 + -0.7 554,760 - -0.8 554,760 - -0.8 555,622 - +0.3 555,683 - +0.4 555,683 - -0.7 555,724 + PS417_02425 0.12 +0.5 555,725 - PS417_02425 0.12 +0.8 555,986 - PS417_02425 0.44 +1.6 556,115 - PS417_02425 0.60 -0.6 556,193 - PS417_02425 0.69 -0.4 556,193 - PS417_02425 0.69 +1.6 556,193 - PS417_02425 0.69 +0.7 556,557 - -0.4 556,775 + PS417_02430 0.18 +0.5 556,776 - PS417_02430 0.18 +0.4 556,778 + PS417_02430 0.18 +0.3 556,779 - PS417_02430 0.18 +0.2 556,779 - PS417_02430 0.18 -1.5 556,973 + PS417_02430 0.31 +0.7 557,049 + PS417_02430 0.36 +1.0 557,049 + PS417_02430 0.36 +0.5 557,049 + PS417_02430 0.36 -0.1 557,050 - +0.6
Or see this region's nucleotide sequence