Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02260 and PS417_02265 are separated by 118 nucleotides PS417_02265 and PS417_02270 are separated by 675 nucleotides
PS417_02260: PS417_02260 - serine/threonine protein kinase, at 513,857 to 515,296
_02260
PS417_02265: PS417_02265 - TonB-dependent receptor, at 515,415 to 517,499
_02265
PS417_02270: PS417_02270 - carbamoyltransferase, at 518,175 to 519,932
_02270
Position (kb) 515
516
517
518 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 515.333 kb on + strand at 515.348 kb on - strand at 515.350 kb on + strand at 515.350 kb on + strand at 515.350 kb on + strand at 515.350 kb on + strand at 515.350 kb on + strand at 515.351 kb on - strand at 515.351 kb on - strand at 515.473 kb on + strand at 515.473 kb on + strand at 515.473 kb on + strand at 515.473 kb on + strand at 515.474 kb on - strand at 515.477 kb on - strand at 515.515 kb on + strand at 515.600 kb on + strand at 515.601 kb on - strand at 515.650 kb on + strand, within PS417_02265 at 515.650 kb on + strand, within PS417_02265 at 515.651 kb on - strand, within PS417_02265 at 515.991 kb on - strand, within PS417_02265 at 515.991 kb on - strand, within PS417_02265 at 516.012 kb on + strand, within PS417_02265 at 516.026 kb on - strand, within PS417_02265 at 516.136 kb on + strand, within PS417_02265 at 516.155 kb on + strand, within PS417_02265 at 516.163 kb on + strand, within PS417_02265 at 516.360 kb on + strand, within PS417_02265 at 516.581 kb on - strand, within PS417_02265 at 516.608 kb on - strand, within PS417_02265 at 516.608 kb on - strand, within PS417_02265 at 516.695 kb on - strand, within PS417_02265 at 516.715 kb on - strand, within PS417_02265 at 516.736 kb on + strand, within PS417_02265 at 516.736 kb on + strand, within PS417_02265 at 516.736 kb on + strand, within PS417_02265 at 516.793 kb on + strand, within PS417_02265 at 516.793 kb on + strand, within PS417_02265 at 517.060 kb on + strand, within PS417_02265 at 517.060 kb on + strand, within PS417_02265 at 517.246 kb on + strand, within PS417_02265 at 517.247 kb on - strand, within PS417_02265 at 517.247 kb on - strand, within PS417_02265 at 517.464 kb on + strand at 517.532 kb on + strand at 517.533 kb on - strand at 517.631 kb on - strand at 517.633 kb on + strand at 517.633 kb on + strand at 517.672 kb on + strand at 517.689 kb on + strand at 517.689 kb on + strand at 517.705 kb on + strand at 517.706 kb on - strand at 517.714 kb on + strand at 517.715 kb on - strand at 517.746 kb on + strand at 517.746 kb on + strand at 517.747 kb on - strand at 517.994 kb on + strand at 517.995 kb on - strand at 518.027 kb on - strand at 518.079 kb on - strand at 518.147 kb on + strand at 518.147 kb on + strand at 518.156 kb on + strand at 518.156 kb on + strand at 518.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 515,333 + -2.1 515,348 - -0.6 515,350 + +0.2 515,350 + +0.4 515,350 + -1.4 515,350 + +1.5 515,350 + -0.3 515,351 - -0.9 515,351 - +0.2 515,473 + -0.7 515,473 + -0.3 515,473 + +0.3 515,473 + +0.1 515,474 - +0.4 515,477 - -0.1 515,515 + +0.2 515,600 + -0.5 515,601 - +1.0 515,650 + PS417_02265 0.11 +0.1 515,650 + PS417_02265 0.11 +0.5 515,651 - PS417_02265 0.11 +1.0 515,991 - PS417_02265 0.28 +0.5 515,991 - PS417_02265 0.28 -0.3 516,012 + PS417_02265 0.29 +0.5 516,026 - PS417_02265 0.29 +0.3 516,136 + PS417_02265 0.35 +0.8 516,155 + PS417_02265 0.35 -0.4 516,163 + PS417_02265 0.36 +0.4 516,360 + PS417_02265 0.45 -1.3 516,581 - PS417_02265 0.56 +1.3 516,608 - PS417_02265 0.57 +0.3 516,608 - PS417_02265 0.57 +0.1 516,695 - PS417_02265 0.61 +0.1 516,715 - PS417_02265 0.62 +0.6 516,736 + PS417_02265 0.63 -0.0 516,736 + PS417_02265 0.63 -2.3 516,736 + PS417_02265 0.63 +0.3 516,793 + PS417_02265 0.66 +2.0 516,793 + PS417_02265 0.66 +0.1 517,060 + PS417_02265 0.79 +2.1 517,060 + PS417_02265 0.79 +0.9 517,246 + PS417_02265 0.88 +3.6 517,247 - PS417_02265 0.88 -0.1 517,247 - PS417_02265 0.88 -0.4 517,464 + -1.2 517,532 + +0.2 517,533 - +0.1 517,631 - -1.9 517,633 + -1.2 517,633 + -1.7 517,672 + -0.1 517,689 + -0.4 517,689 + +0.2 517,705 + +0.0 517,706 - +0.6 517,714 + -0.5 517,715 - +0.6 517,746 + -0.3 517,746 + +1.1 517,747 - -2.7 517,994 + -1.5 517,995 - -0.8 518,027 - -0.4 518,079 - -1.5 518,147 + +0.7 518,147 + +0.3 518,156 + +1.7 518,156 + +1.0 518,235 + +3.8
Or see this region's nucleotide sequence