Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16555 and PS417_16560 overlap by 8 nucleotides PS417_16560 and PS417_16565 are separated by 231 nucleotides PS417_16565 and PS417_16570 are separated by 489 nucleotides
PS417_16555: PS417_16555 - cupin, at 3,617,592 to 3,618,758
_16555
PS417_16560: PS417_16560 - GNAT family acetyltransferase, at 3,618,751 to 3,619,173
_16560
PS417_16565: PS417_16565 - secretin, at 3,619,405 to 3,620,142
_16565
PS417_16570: PS417_16570 - isocitrate lyase, at 3,620,632 to 3,621,957
_16570
Position (kb)
3619
3620
3621 Strain fitness (log2 ratio)
-1
0
1
2 at 3618.767 kb on + strand at 3618.767 kb on + strand at 3618.768 kb on - strand at 3618.768 kb on - strand at 3618.768 kb on - strand at 3618.768 kb on - strand at 3618.768 kb on - strand at 3618.829 kb on - strand, within PS417_16560 at 3619.076 kb on - strand, within PS417_16560 at 3619.091 kb on - strand, within PS417_16560 at 3619.240 kb on + strand at 3619.240 kb on + strand at 3619.240 kb on + strand at 3619.280 kb on + strand at 3619.280 kb on + strand at 3619.281 kb on - strand at 3619.281 kb on - strand at 3619.281 kb on - strand at 3619.281 kb on - strand at 3619.281 kb on - strand at 3619.372 kb on - strand at 3619.372 kb on - strand at 3619.720 kb on + strand, within PS417_16565 at 3619.794 kb on - strand, within PS417_16565 at 3619.885 kb on + strand, within PS417_16565 at 3619.886 kb on - strand, within PS417_16565 at 3619.886 kb on - strand, within PS417_16565 at 3619.970 kb on + strand, within PS417_16565 at 3619.971 kb on - strand, within PS417_16565 at 3620.061 kb on + strand, within PS417_16565 at 3620.071 kb on - strand at 3620.071 kb on - strand at 3620.083 kb on + strand at 3620.084 kb on - strand at 3620.170 kb on - strand at 3620.172 kb on + strand at 3620.173 kb on - strand at 3620.173 kb on - strand at 3620.186 kb on - strand at 3620.297 kb on - strand at 3620.301 kb on + strand at 3620.327 kb on + strand at 3620.468 kb on + strand at 3620.472 kb on + strand at 3620.920 kb on + strand, within PS417_16570 at 3620.921 kb on - strand, within PS417_16570 at 3620.921 kb on - strand, within PS417_16570 at 3620.921 kb on - strand, within PS417_16570 at 3620.971 kb on + strand, within PS417_16570 at 3620.971 kb on + strand, within PS417_16570 at 3620.971 kb on + strand, within PS417_16570 at 3621.094 kb on + strand, within PS417_16570 at 3621.095 kb on - strand, within PS417_16570 at 3621.114 kb on + strand, within PS417_16570
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,618,767 + +0.2 3,618,767 + -0.3 3,618,768 - +0.5 3,618,768 - +1.3 3,618,768 - -0.7 3,618,768 - +0.6 3,618,768 - -0.8 3,618,829 - PS417_16560 0.18 +0.7 3,619,076 - PS417_16560 0.77 +1.8 3,619,091 - PS417_16560 0.80 +0.2 3,619,240 + -0.1 3,619,240 + -0.7 3,619,240 + +0.4 3,619,280 + +0.0 3,619,280 + -1.7 3,619,281 - -0.5 3,619,281 - -0.5 3,619,281 - -0.3 3,619,281 - +0.4 3,619,281 - +0.0 3,619,372 - -0.5 3,619,372 - -1.5 3,619,720 + PS417_16565 0.43 -0.2 3,619,794 - PS417_16565 0.53 -1.1 3,619,885 + PS417_16565 0.65 -0.9 3,619,886 - PS417_16565 0.65 -1.1 3,619,886 - PS417_16565 0.65 -1.2 3,619,970 + PS417_16565 0.77 -1.3 3,619,971 - PS417_16565 0.77 -0.3 3,620,061 + PS417_16565 0.89 -1.5 3,620,071 - +1.1 3,620,071 - -0.1 3,620,083 + -1.7 3,620,084 - -0.8 3,620,170 - -0.0 3,620,172 + -1.0 3,620,173 - +0.8 3,620,173 - +0.2 3,620,186 - -1.2 3,620,297 - +1.3 3,620,301 + +0.7 3,620,327 + -1.0 3,620,468 + -1.6 3,620,472 + -1.1 3,620,920 + PS417_16570 0.22 +0.1 3,620,921 - PS417_16570 0.22 -0.0 3,620,921 - PS417_16570 0.22 -0.3 3,620,921 - PS417_16570 0.22 -1.1 3,620,971 + PS417_16570 0.26 -0.8 3,620,971 + PS417_16570 0.26 +1.2 3,620,971 + PS417_16570 0.26 -1.0 3,621,094 + PS417_16570 0.35 -0.3 3,621,095 - PS417_16570 0.35 +0.2 3,621,114 + PS417_16570 0.36 +0.2
Or see this region's nucleotide sequence