Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12825 and PS417_12830 are separated by 113 nucleotides PS417_12830 and PS417_12835 are separated by 20 nucleotides PS417_12835 and PS417_12840 are separated by 113 nucleotides
PS417_12825: PS417_12825 - MFS transporter, at 2,806,926 to 2,808,140
_12825
PS417_12830: PS417_12830 - LysR family transcriptional regulator, at 2,808,254 to 2,809,162
_12830
PS417_12835: PS417_12835 - GNAT family acetyltransferase, at 2,809,183 to 2,809,851
_12835
PS417_12840: PS417_12840 - TetR family transcriptional regulator, at 2,809,965 to 2,810,558
_12840
Position (kb)
2808
2809
2810 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2807.254 kb on + strand, within PS417_12825 at 2807.255 kb on - strand, within PS417_12825 at 2807.262 kb on + strand, within PS417_12825 at 2807.263 kb on - strand, within PS417_12825 at 2807.452 kb on + strand, within PS417_12825 at 2807.452 kb on + strand, within PS417_12825 at 2807.452 kb on + strand, within PS417_12825 at 2807.538 kb on + strand, within PS417_12825 at 2807.599 kb on + strand, within PS417_12825 at 2807.599 kb on + strand, within PS417_12825 at 2807.599 kb on + strand, within PS417_12825 at 2808.177 kb on + strand at 2808.189 kb on + strand at 2808.223 kb on - strand at 2808.241 kb on - strand at 2808.241 kb on - strand at 2808.242 kb on + strand at 2808.514 kb on + strand, within PS417_12830 at 2808.514 kb on + strand, within PS417_12830 at 2808.514 kb on + strand, within PS417_12830 at 2808.515 kb on - strand, within PS417_12830 at 2808.833 kb on + strand, within PS417_12830 at 2808.834 kb on - strand, within PS417_12830 at 2808.834 kb on - strand at 2808.834 kb on - strand, within PS417_12830 at 2808.834 kb on - strand, within PS417_12830 at 2808.837 kb on + strand, within PS417_12830 at 2808.876 kb on + strand, within PS417_12830 at 2808.986 kb on + strand, within PS417_12830 at 2808.987 kb on - strand, within PS417_12830 at 2809.143 kb on + strand at 2809.143 kb on + strand at 2809.144 kb on - strand at 2809.201 kb on + strand at 2809.306 kb on + strand, within PS417_12835 at 2809.315 kb on + strand, within PS417_12835 at 2809.322 kb on - strand, within PS417_12835 at 2809.348 kb on + strand, within PS417_12835 at 2809.435 kb on + strand, within PS417_12835 at 2809.488 kb on - strand, within PS417_12835 at 2809.735 kb on - strand, within PS417_12835 at 2809.735 kb on - strand, within PS417_12835 at 2809.735 kb on - strand, within PS417_12835 at 2809.738 kb on + strand, within PS417_12835 at 2809.738 kb on + strand at 2809.738 kb on + strand, within PS417_12835 at 2809.739 kb on - strand, within PS417_12835 at 2809.739 kb on - strand, within PS417_12835 at 2810.141 kb on + strand, within PS417_12840
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,807,254 + PS417_12825 0.27 -0.1 2,807,255 - PS417_12825 0.27 +0.1 2,807,262 + PS417_12825 0.28 -0.0 2,807,263 - PS417_12825 0.28 +0.0 2,807,452 + PS417_12825 0.43 -0.4 2,807,452 + PS417_12825 0.43 +0.6 2,807,452 + PS417_12825 0.43 -0.1 2,807,538 + PS417_12825 0.50 +0.1 2,807,599 + PS417_12825 0.55 -0.1 2,807,599 + PS417_12825 0.55 -0.3 2,807,599 + PS417_12825 0.55 -1.1 2,808,177 + -0.8 2,808,189 + +0.1 2,808,223 - -1.0 2,808,241 - -0.5 2,808,241 - +0.6 2,808,242 + -0.2 2,808,514 + PS417_12830 0.29 -1.1 2,808,514 + PS417_12830 0.29 -1.3 2,808,514 + PS417_12830 0.29 -0.9 2,808,515 - PS417_12830 0.29 -1.8 2,808,833 + PS417_12830 0.64 +0.0 2,808,834 - PS417_12830 0.64 +0.1 2,808,834 - +0.8 2,808,834 - PS417_12830 0.64 -0.7 2,808,834 - PS417_12830 0.64 -1.2 2,808,837 + PS417_12830 0.64 +0.4 2,808,876 + PS417_12830 0.68 -1.1 2,808,986 + PS417_12830 0.81 +0.5 2,808,987 - PS417_12830 0.81 -2.5 2,809,143 + -1.6 2,809,143 + +0.4 2,809,144 - +0.5 2,809,201 + -0.2 2,809,306 + PS417_12835 0.18 -0.5 2,809,315 + PS417_12835 0.20 +0.4 2,809,322 - PS417_12835 0.21 -0.9 2,809,348 + PS417_12835 0.25 +1.5 2,809,435 + PS417_12835 0.38 -0.6 2,809,488 - PS417_12835 0.46 -1.2 2,809,735 - PS417_12835 0.83 -0.5 2,809,735 - PS417_12835 0.83 +0.6 2,809,735 - PS417_12835 0.83 -0.2 2,809,738 + PS417_12835 0.83 -0.7 2,809,738 + -0.2 2,809,738 + PS417_12835 0.83 -0.1 2,809,739 - PS417_12835 0.83 -0.1 2,809,739 - PS417_12835 0.83 +1.9 2,810,141 + PS417_12840 0.30 +0.9
Or see this region's nucleotide sequence