Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11625 and PS417_11630 are separated by 12 nucleotides PS417_11630 and PS417_11635 are separated by 179 nucleotides
PS417_11625: PS417_11625 - fumarate hydratase, at 2,500,359 to 2,501,753
_11625
PS417_11630: PS417_11630 - sodium:dicarboxylate symporter, at 2,501,766 to 2,503,070
_11630
PS417_11635: PS417_11635 - 3-carboxy-cis,cis-muconate cycloisomerase, at 2,503,250 to 2,504,605
_11635
Position (kb)
2501
2502
2503
2504 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2500.932 kb on + strand, within PS417_11625 at 2500.933 kb on - strand, within PS417_11625 at 2500.933 kb on - strand, within PS417_11625 at 2501.045 kb on + strand, within PS417_11625 at 2501.046 kb on - strand, within PS417_11625 at 2501.806 kb on + strand at 2502.124 kb on + strand at 2502.125 kb on - strand, within PS417_11630 at 2502.283 kb on + strand, within PS417_11630 at 2502.283 kb on + strand, within PS417_11630 at 2502.283 kb on + strand, within PS417_11630 at 2502.283 kb on + strand, within PS417_11630 at 2502.283 kb on + strand, within PS417_11630 at 2502.284 kb on - strand, within PS417_11630 at 2502.324 kb on + strand, within PS417_11630 at 2502.348 kb on - strand, within PS417_11630 at 2502.591 kb on + strand, within PS417_11630 at 2502.591 kb on + strand, within PS417_11630 at 2502.649 kb on + strand, within PS417_11630 at 2502.649 kb on + strand, within PS417_11630 at 2502.649 kb on + strand, within PS417_11630 at 2502.817 kb on + strand, within PS417_11630 at 2503.039 kb on + strand at 2503.040 kb on - strand at 2503.091 kb on + strand at 2503.091 kb on + strand at 2503.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,500,932 + PS417_11625 0.41 +0.3 2,500,933 - PS417_11625 0.41 +0.7 2,500,933 - PS417_11625 0.41 +0.2 2,501,045 + PS417_11625 0.49 -0.7 2,501,046 - PS417_11625 0.49 +0.6 2,501,806 + +0.5 2,502,124 + -0.8 2,502,125 - PS417_11630 0.28 +1.0 2,502,283 + PS417_11630 0.40 -0.5 2,502,283 + PS417_11630 0.40 +0.8 2,502,283 + PS417_11630 0.40 +0.5 2,502,283 + PS417_11630 0.40 -0.8 2,502,283 + PS417_11630 0.40 +0.9 2,502,284 - PS417_11630 0.40 +1.1 2,502,324 + PS417_11630 0.43 +0.4 2,502,348 - PS417_11630 0.45 +1.6 2,502,591 + PS417_11630 0.63 -0.1 2,502,591 + PS417_11630 0.63 +2.8 2,502,649 + PS417_11630 0.68 +0.5 2,502,649 + PS417_11630 0.68 +0.2 2,502,649 + PS417_11630 0.68 +0.6 2,502,817 + PS417_11630 0.81 -0.3 2,503,039 + -2.4 2,503,040 - -0.2 2,503,091 + -0.1 2,503,091 + -0.2 2,503,105 + -0.2
Or see this region's nucleotide sequence