Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05895 and PS417_05900 are separated by 2 nucleotides PS417_05900 and PS417_05905 are separated by 255 nucleotides PS417_05905 and PS417_05910 are separated by 100 nucleotides
PS417_05895: PS417_05895 - membrane protein, at 1,309,575 to 1,310,744
_05895
PS417_05900: PS417_05900 - TetR family transcriptional regulator, at 1,310,747 to 1,311,343
_05900
PS417_05905: PS417_05905 - hypothetical protein, at 1,311,599 to 1,311,784
_05905
PS417_05910: PS417_05910 - glucan biosynthesis protein D, at 1,311,885 to 1,313,510
_05910
Position (kb)
1311
1312 Strain fitness (log2 ratio)
-2
-1
0
1 at 1310.621 kb on - strand, within PS417_05895 at 1310.673 kb on - strand at 1310.673 kb on - strand at 1311.193 kb on - strand, within PS417_05900 at 1311.193 kb on - strand, within PS417_05900 at 1311.344 kb on - strand at 1311.474 kb on + strand at 1311.474 kb on + strand at 1311.480 kb on + strand at 1311.485 kb on + strand at 1311.485 kb on + strand at 1311.485 kb on + strand at 1311.485 kb on + strand at 1311.485 kb on + strand at 1311.485 kb on + strand at 1311.486 kb on - strand at 1311.570 kb on + strand at 1311.571 kb on - strand at 1311.571 kb on - strand at 1311.612 kb on - strand at 1311.619 kb on - strand, within PS417_05905 at 1311.746 kb on + strand, within PS417_05905 at 1311.874 kb on - strand at 1311.893 kb on - strand at 1311.897 kb on - strand at 1311.923 kb on - strand at 1311.923 kb on - strand at 1311.928 kb on + strand at 1311.928 kb on + strand at 1311.928 kb on + strand at 1311.928 kb on + strand at 1311.929 kb on - strand at 1311.929 kb on - strand at 1311.929 kb on - strand at 1312.239 kb on - strand, within PS417_05910 at 1312.262 kb on + strand, within PS417_05910 at 1312.262 kb on + strand, within PS417_05910 at 1312.263 kb on - strand, within PS417_05910 at 1312.267 kb on - strand, within PS417_05910 at 1312.297 kb on + strand, within PS417_05910 at 1312.297 kb on + strand, within PS417_05910 at 1312.297 kb on + strand, within PS417_05910 at 1312.298 kb on - strand, within PS417_05910 at 1312.303 kb on + strand, within PS417_05910 at 1312.304 kb on - strand, within PS417_05910 at 1312.383 kb on - strand, within PS417_05910 at 1312.460 kb on - strand, within PS417_05910 at 1312.480 kb on + strand, within PS417_05910 at 1312.480 kb on + strand, within PS417_05910 at 1312.658 kb on + strand, within PS417_05910 at 1312.659 kb on - strand, within PS417_05910
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,310,621 - PS417_05895 0.89 -1.3 1,310,673 - -0.3 1,310,673 - +1.0 1,311,193 - PS417_05900 0.75 -0.2 1,311,193 - PS417_05900 0.75 -1.0 1,311,344 - -0.3 1,311,474 + -0.8 1,311,474 + -0.7 1,311,480 + -0.1 1,311,485 + -0.5 1,311,485 + +0.0 1,311,485 + +0.0 1,311,485 + -0.3 1,311,485 + -0.4 1,311,485 + -0.3 1,311,486 - -0.3 1,311,570 + +0.5 1,311,571 - -0.4 1,311,571 - -0.7 1,311,612 - +0.3 1,311,619 - PS417_05905 0.11 +0.8 1,311,746 + PS417_05905 0.79 +0.5 1,311,874 - +0.4 1,311,893 - +0.6 1,311,897 - -0.6 1,311,923 - -0.6 1,311,923 - -1.0 1,311,928 + +0.9 1,311,928 + -1.1 1,311,928 + -0.5 1,311,928 + +0.4 1,311,929 - +0.5 1,311,929 - -1.2 1,311,929 - -1.8 1,312,239 - PS417_05910 0.22 -1.5 1,312,262 + PS417_05910 0.23 -0.9 1,312,262 + PS417_05910 0.23 +0.4 1,312,263 - PS417_05910 0.23 -0.4 1,312,267 - PS417_05910 0.23 -1.3 1,312,297 + PS417_05910 0.25 -2.3 1,312,297 + PS417_05910 0.25 -0.6 1,312,297 + PS417_05910 0.25 -1.1 1,312,298 - PS417_05910 0.25 -0.4 1,312,303 + PS417_05910 0.26 -0.9 1,312,304 - PS417_05910 0.26 -0.9 1,312,383 - PS417_05910 0.31 -1.3 1,312,460 - PS417_05910 0.35 -1.6 1,312,480 + PS417_05910 0.37 -1.2 1,312,480 + PS417_05910 0.37 -0.4 1,312,658 + PS417_05910 0.48 -0.0 1,312,659 - PS417_05910 0.48 -0.5
Or see this region's nucleotide sequence