Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05270 and PS417_05275 are separated by 13 nucleotides PS417_05275 and PS417_05285 are separated by 87 nucleotides PS417_05285 and PS417_05290 overlap by 4 nucleotides
PS417_05270: PS417_05270 - 3-oxoacyl-ACP reductase, at 1,169,373 to 1,170,122
_05270
PS417_05275: PS417_05275 - hypothetical protein, at 1,170,136 to 1,170,429
_05275
PS417_05285: PS417_05285 - cobalamin biosynthesis protein CobW, at 1,170,517 to 1,171,491
_05285
PS417_05290: PS417_05290 - hypothetical protein, at 1,171,488 to 1,172,663
_05290
Position (kb)
1170
1171
1172 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1169.803 kb on + strand, within PS417_05270 at 1169.803 kb on + strand, within PS417_05270 at 1169.803 kb on + strand, within PS417_05270 at 1169.804 kb on - strand, within PS417_05270 at 1169.804 kb on - strand, within PS417_05270 at 1169.804 kb on - strand, within PS417_05270 at 1169.804 kb on - strand, within PS417_05270 at 1169.804 kb on - strand, within PS417_05270 at 1169.807 kb on + strand, within PS417_05270 at 1169.807 kb on + strand, within PS417_05270 at 1169.808 kb on - strand, within PS417_05270 at 1169.808 kb on - strand, within PS417_05270 at 1170.131 kb on + strand at 1170.132 kb on - strand at 1170.428 kb on - strand at 1170.428 kb on - strand at 1170.489 kb on + strand at 1170.656 kb on + strand, within PS417_05285 at 1170.656 kb on + strand, within PS417_05285 at 1170.656 kb on + strand, within PS417_05285 at 1170.657 kb on - strand, within PS417_05285 at 1170.687 kb on - strand, within PS417_05285 at 1170.687 kb on - strand, within PS417_05285 at 1171.262 kb on + strand, within PS417_05285 at 1171.499 kb on - strand at 1171.511 kb on + strand at 1171.578 kb on + strand at 1171.578 kb on + strand at 1171.635 kb on + strand, within PS417_05290 at 1172.257 kb on - strand, within PS417_05290 at 1172.355 kb on + strand, within PS417_05290 at 1172.355 kb on + strand, within PS417_05290 at 1172.355 kb on + strand, within PS417_05290 at 1172.356 kb on - strand, within PS417_05290 at 1172.412 kb on + strand, within PS417_05290 at 1172.412 kb on + strand, within PS417_05290 at 1172.412 kb on + strand, within PS417_05290 at 1172.412 kb on + strand, within PS417_05290 at 1172.412 kb on + strand, within PS417_05290 at 1172.412 kb on + strand, within PS417_05290 at 1172.413 kb on - strand, within PS417_05290 at 1172.413 kb on - strand, within PS417_05290
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,169,803 + PS417_05270 0.57 +1.0 1,169,803 + PS417_05270 0.57 +1.3 1,169,803 + PS417_05270 0.57 -1.2 1,169,804 - PS417_05270 0.57 +0.3 1,169,804 - PS417_05270 0.57 +0.3 1,169,804 - PS417_05270 0.57 +1.6 1,169,804 - PS417_05270 0.57 +0.8 1,169,804 - PS417_05270 0.57 +0.6 1,169,807 + PS417_05270 0.58 -0.4 1,169,807 + PS417_05270 0.58 -0.7 1,169,808 - PS417_05270 0.58 +0.9 1,169,808 - PS417_05270 0.58 +0.6 1,170,131 + -0.2 1,170,132 - -1.0 1,170,428 - +0.7 1,170,428 - -0.7 1,170,489 + -0.4 1,170,656 + PS417_05285 0.14 -0.2 1,170,656 + PS417_05285 0.14 +0.8 1,170,656 + PS417_05285 0.14 -0.4 1,170,657 - PS417_05285 0.14 +0.6 1,170,687 - PS417_05285 0.17 -0.6 1,170,687 - PS417_05285 0.17 +0.7 1,171,262 + PS417_05285 0.76 +3.1 1,171,499 - -0.3 1,171,511 + +1.3 1,171,578 + +0.2 1,171,578 + +0.6 1,171,635 + PS417_05290 0.12 -0.4 1,172,257 - PS417_05290 0.65 -0.4 1,172,355 + PS417_05290 0.74 +0.3 1,172,355 + PS417_05290 0.74 -0.0 1,172,355 + PS417_05290 0.74 +0.7 1,172,356 - PS417_05290 0.74 +0.3 1,172,412 + PS417_05290 0.79 -0.6 1,172,412 + PS417_05290 0.79 +0.5 1,172,412 + PS417_05290 0.79 -1.6 1,172,412 + PS417_05290 0.79 +0.4 1,172,412 + PS417_05290 0.79 -0.4 1,172,412 + PS417_05290 0.79 +0.1 1,172,413 - PS417_05290 0.79 +0.0 1,172,413 - PS417_05290 0.79 -1.0
Or see this region's nucleotide sequence