Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04840 and PS417_04845 are separated by 74 nucleotides PS417_04845 and PS417_04850 are separated by 836 nucleotides
PS417_04840: PS417_04840 - glycosyl transferase, at 1,074,189 to 1,075,679
_04840
PS417_04845: PS417_04845 - GDP-mannose dehydrogenase, at 1,075,754 to 1,077,070
_04845
PS417_04850: PS417_04850 - hypothetical protein, at 1,077,907 to 1,078,686
_04850
Position (kb)
1075
1076
1077
1078 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1074.876 kb on + strand, within PS417_04840 at 1074.876 kb on + strand at 1074.877 kb on - strand, within PS417_04840 at 1074.877 kb on - strand at 1074.900 kb on - strand, within PS417_04840 at 1074.900 kb on - strand at 1075.080 kb on + strand, within PS417_04840 at 1075.351 kb on + strand at 1075.462 kb on + strand, within PS417_04840 at 1075.462 kb on + strand, within PS417_04840 at 1075.472 kb on - strand, within PS417_04840 at 1075.502 kb on + strand, within PS417_04840 at 1075.550 kb on + strand at 1075.577 kb on + strand at 1075.577 kb on + strand at 1075.578 kb on - strand at 1075.578 kb on - strand at 1075.650 kb on + strand at 1075.651 kb on - strand at 1075.651 kb on - strand at 1075.651 kb on - strand at 1075.659 kb on + strand at 1075.670 kb on + strand at 1075.670 kb on + strand at 1075.670 kb on + strand at 1075.723 kb on + strand at 1075.755 kb on + strand at 1075.766 kb on - strand at 1076.017 kb on - strand, within PS417_04845 at 1076.017 kb on - strand, within PS417_04845 at 1076.168 kb on + strand, within PS417_04845 at 1076.301 kb on + strand, within PS417_04845 at 1076.301 kb on + strand, within PS417_04845 at 1076.302 kb on - strand, within PS417_04845 at 1076.302 kb on - strand, within PS417_04845 at 1076.304 kb on + strand, within PS417_04845 at 1076.568 kb on + strand, within PS417_04845 at 1076.568 kb on + strand, within PS417_04845 at 1076.569 kb on - strand, within PS417_04845 at 1076.778 kb on + strand, within PS417_04845 at 1076.985 kb on - strand at 1077.056 kb on + strand at 1077.057 kb on - strand at 1077.077 kb on + strand at 1077.078 kb on - strand at 1077.458 kb on - strand at 1077.674 kb on - strand at 1077.674 kb on - strand at 1077.714 kb on + strand at 1077.715 kb on - strand at 1077.878 kb on + strand at 1077.974 kb on + strand at 1078.067 kb on + strand, within PS417_04850 at 1078.068 kb on - strand, within PS417_04850 at 1078.068 kb on - strand, within PS417_04850 at 1078.068 kb on - strand, within PS417_04850
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,074,876 + PS417_04840 0.46 -0.8 1,074,876 + +1.7 1,074,877 - PS417_04840 0.46 +0.8 1,074,877 - +2.7 1,074,900 - PS417_04840 0.48 +1.7 1,074,900 - -0.2 1,075,080 + PS417_04840 0.60 -0.0 1,075,351 + +0.1 1,075,462 + PS417_04840 0.85 -0.3 1,075,462 + PS417_04840 0.85 +0.1 1,075,472 - PS417_04840 0.86 -0.3 1,075,502 + PS417_04840 0.88 +0.3 1,075,550 + -0.5 1,075,577 + +0.6 1,075,577 + +0.5 1,075,578 - -0.4 1,075,578 - +0.2 1,075,650 + -0.2 1,075,651 - +0.2 1,075,651 - +1.4 1,075,651 - +0.8 1,075,659 + -0.2 1,075,670 + -0.8 1,075,670 + -0.8 1,075,670 + +0.6 1,075,723 + -0.4 1,075,755 + +0.4 1,075,766 - +0.7 1,076,017 - PS417_04845 0.20 +0.7 1,076,017 - PS417_04845 0.20 +0.7 1,076,168 + PS417_04845 0.31 -0.8 1,076,301 + PS417_04845 0.42 -0.0 1,076,301 + PS417_04845 0.42 +0.4 1,076,302 - PS417_04845 0.42 +0.6 1,076,302 - PS417_04845 0.42 +0.9 1,076,304 + PS417_04845 0.42 +0.7 1,076,568 + PS417_04845 0.62 +0.8 1,076,568 + PS417_04845 0.62 +0.1 1,076,569 - PS417_04845 0.62 +0.6 1,076,778 + PS417_04845 0.78 -0.0 1,076,985 - -0.8 1,077,056 + +0.5 1,077,057 - -1.2 1,077,077 + +0.1 1,077,078 - +2.3 1,077,458 - +1.0 1,077,674 - +1.4 1,077,674 - +0.8 1,077,714 + -2.6 1,077,715 - -1.9 1,077,878 + +1.5 1,077,974 + +0.6 1,078,067 + PS417_04850 0.21 -0.0 1,078,068 - PS417_04850 0.21 -0.4 1,078,068 - PS417_04850 0.21 -0.1 1,078,068 - PS417_04850 0.21 -1.0
Or see this region's nucleotide sequence