Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22485 and PS417_22490 are separated by 69 nucleotides PS417_22490 and PS417_22495 are separated by 3 nucleotides PS417_22495 and PS417_22500 are separated by 886 nucleotides
PS417_22485: PS417_22485 - cold-shock protein, at 4,867,692 to 4,868,294
_22485
PS417_22490: PS417_22490 - hypothetical protein, at 4,868,364 to 4,868,570
_22490
PS417_22495: PS417_22495 - histidine triad (HIT) protein, at 4,868,574 to 4,868,999
_22495
PS417_22500: PS417_22500 - porin, at 4,869,886 to 4,871,184
_22500
Position (kb)
4868
4869 Strain fitness (log2 ratio)
-1
0
1 at 4867.674 kb on + strand at 4868.362 kb on + strand at 4868.365 kb on + strand at 4868.366 kb on - strand at 4868.368 kb on + strand at 4868.368 kb on + strand at 4868.368 kb on + strand at 4868.369 kb on - strand at 4868.369 kb on - strand at 4868.654 kb on - strand at 4868.745 kb on + strand, within PS417_22495 at 4868.745 kb on + strand, within PS417_22495 at 4868.746 kb on - strand, within PS417_22495 at 4868.988 kb on + strand at 4868.989 kb on - strand at 4869.364 kb on + strand at 4869.365 kb on - strand at 4869.418 kb on + strand at 4869.615 kb on - strand at 4869.718 kb on + strand at 4869.794 kb on - strand at 4869.794 kb on - strand at 4869.936 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,867,674 + -0.3 4,868,362 + +0.4 4,868,365 + -1.2 4,868,366 - -0.5 4,868,368 + +0.9 4,868,368 + -0.7 4,868,368 + -0.2 4,868,369 - -1.4 4,868,369 - +0.0 4,868,654 - +1.1 4,868,745 + PS417_22495 0.40 +1.0 4,868,745 + PS417_22495 0.40 +1.5 4,868,746 - PS417_22495 0.40 +1.1 4,868,988 + +1.2 4,868,989 - -0.4 4,869,364 + -0.1 4,869,365 - +0.3 4,869,418 + +0.1 4,869,615 - +1.1 4,869,718 + -0.2 4,869,794 - +1.5 4,869,794 - -0.2 4,869,936 + -0.2
Or see this region's nucleotide sequence