Strain Fitness in Pseudomonas simiae WCS417 around PS417_20745

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_20735 and PS417_20740 are separated by 172 nucleotidesPS417_20740 and PS417_20745 overlap by 1 nucleotidesPS417_20745 and PS417_20750 are separated by 207 nucleotides PS417_20735: PS417_20735 - cytochrome Cbb3, at 4,505,998 to 4,506,975 _20735 PS417_20740: PS417_20740 - prevent-host-death protein, at 4,507,148 to 4,507,402 _20740 PS417_20745: PS417_20745 - twitching motility protein PilT, at 4,507,402 to 4,507,812 _20745 PS417_20750: PS417_20750 - (Fe-S)-binding protein, at 4,508,020 to 4,509,447 _20750 Position (kb) 4507 4508Strain fitness (log2 ratio) -2 -1 0 1at 4506.818 kb on - strand, within PS417_20735at 4506.929 kb on - strandat 4506.929 kb on - strandat 4506.929 kb on - strandat 4506.935 kb on - strandat 4506.973 kb on + strandat 4507.075 kb on - strandat 4507.114 kb on + strandat 4507.260 kb on + strand, within PS417_20740at 4507.260 kb on + strand, within PS417_20740at 4507.261 kb on - strand, within PS417_20740at 4507.261 kb on - strand, within PS417_20740at 4507.261 kb on - strand, within PS417_20740at 4507.261 kb on - strand, within PS417_20740at 4507.267 kb on - strand, within PS417_20740at 4507.267 kb on - strand, within PS417_20740at 4507.267 kb on - strand, within PS417_20740at 4507.267 kb on - strand, within PS417_20740at 4507.405 kb on + strandat 4507.405 kb on + strandat 4507.406 kb on - strandat 4507.406 kb on - strandat 4507.425 kb on + strandat 4507.426 kb on - strandat 4507.426 kb on - strandat 4507.441 kb on - strandat 4507.515 kb on + strand, within PS417_20745at 4507.515 kb on + strand, within PS417_20745at 4507.516 kb on - strand, within PS417_20745at 4507.607 kb on + strand, within PS417_20745at 4507.607 kb on + strand, within PS417_20745at 4507.607 kb on + strand, within PS417_20745at 4507.607 kb on + strand, within PS417_20745at 4507.608 kb on - strand, within PS417_20745at 4507.608 kb on - strand, within PS417_20745at 4507.728 kb on - strand, within PS417_20745at 4507.728 kb on - strand, within PS417_20745at 4507.728 kb on - strand, within PS417_20745at 4507.730 kb on + strand, within PS417_20745at 4507.731 kb on - strand, within PS417_20745at 4507.731 kb on - strand, within PS417_20745at 4507.838 kb on + strandat 4507.919 kb on + strandat 4507.933 kb on + strandat 4507.980 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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4,506,818 - PS417_20735 0.84 -0.7
4,506,929 - +0.1
4,506,929 - -1.2
4,506,929 - -0.4
4,506,935 - +1.1
4,506,973 + -0.4
4,507,075 - +0.3
4,507,114 + -0.1
4,507,260 + PS417_20740 0.44 -1.5
4,507,260 + PS417_20740 0.44 -1.4
4,507,261 - PS417_20740 0.44 -0.7
4,507,261 - PS417_20740 0.44 +0.3
4,507,261 - PS417_20740 0.44 +0.1
4,507,261 - PS417_20740 0.44 +0.4
4,507,267 - PS417_20740 0.47 -1.7
4,507,267 - PS417_20740 0.47 -0.2
4,507,267 - PS417_20740 0.47 -1.5
4,507,267 - PS417_20740 0.47 -0.1
4,507,405 + -0.0
4,507,405 + +1.2
4,507,406 - +0.6
4,507,406 - +0.2
4,507,425 + +0.3
4,507,426 - +0.4
4,507,426 - -0.7
4,507,441 - -0.1
4,507,515 + PS417_20745 0.27 -0.6
4,507,515 + PS417_20745 0.27 -1.5
4,507,516 - PS417_20745 0.28 -0.4
4,507,607 + PS417_20745 0.50 -0.0
4,507,607 + PS417_20745 0.50 -1.8
4,507,607 + PS417_20745 0.50 +0.6
4,507,607 + PS417_20745 0.50 +0.1
4,507,608 - PS417_20745 0.50 -0.3
4,507,608 - PS417_20745 0.50 -1.1
4,507,728 - PS417_20745 0.79 -0.5
4,507,728 - PS417_20745 0.79 -1.0
4,507,728 - PS417_20745 0.79 -0.4
4,507,730 + PS417_20745 0.80 -2.1
4,507,731 - PS417_20745 0.80 -2.3
4,507,731 - PS417_20745 0.80 +0.6
4,507,838 + +0.8
4,507,919 + +0.2
4,507,933 + -0.4
4,507,980 + +1.1

Or see this region's nucleotide sequence