Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19460 and PS417_19465 are separated by 166 nucleotides PS417_19465 and PS417_19470 are separated by 3 nucleotides PS417_19470 and PS417_19475 are separated by 96 nucleotides PS417_19475 and PS417_19480 are separated by 171 nucleotides
PS417_19460: PS417_19460 - septum formation inhibitor, at 4,220,114 to 4,220,851
_19460
PS417_19465: PS417_19465 - cell division inhibitor MinD, at 4,221,018 to 4,221,830
_19465
PS417_19470: PS417_19470 - cell division topological specificity factor, at 4,221,834 to 4,222,088
_19470
PS417_19475: PS417_19475 - pseudouridine synthase, at 4,222,185 to 4,222,820
_19475
PS417_19480: PS417_19480 - aminopeptidase, at 4,222,992 to 4,224,281
_19480
Position (kb)
4221
4222
4223 Strain fitness (log2 ratio)
-2
-1
0
1 at 4222.172 kb on - strand at 4222.212 kb on + strand at 4222.661 kb on + strand, within PS417_19475 at 4222.661 kb on + strand, within PS417_19475 at 4222.661 kb on + strand, within PS417_19475 at 4222.661 kb on + strand, within PS417_19475 at 4222.661 kb on + strand, within PS417_19475 at 4222.662 kb on - strand, within PS417_19475 at 4222.662 kb on - strand, within PS417_19475 at 4222.662 kb on - strand, within PS417_19475 at 4222.701 kb on + strand, within PS417_19475 at 4222.701 kb on + strand, within PS417_19475 at 4222.701 kb on + strand, within PS417_19475 at 4222.702 kb on - strand, within PS417_19475 at 4222.702 kb on - strand, within PS417_19475 at 4222.835 kb on + strand at 4222.882 kb on + strand at 4222.987 kb on + strand at 4222.988 kb on - strand at 4222.988 kb on - strand at 4222.988 kb on - strand at 4222.991 kb on + strand at 4222.991 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,222,172 - -0.1 4,222,212 + -0.5 4,222,661 + PS417_19475 0.75 -1.0 4,222,661 + PS417_19475 0.75 -1.4 4,222,661 + PS417_19475 0.75 -1.8 4,222,661 + PS417_19475 0.75 -0.5 4,222,661 + PS417_19475 0.75 -0.5 4,222,662 - PS417_19475 0.75 -0.3 4,222,662 - PS417_19475 0.75 -0.2 4,222,662 - PS417_19475 0.75 -0.2 4,222,701 + PS417_19475 0.81 -0.6 4,222,701 + PS417_19475 0.81 -0.5 4,222,701 + PS417_19475 0.81 -0.5 4,222,702 - PS417_19475 0.81 -2.2 4,222,702 - PS417_19475 0.81 -0.5 4,222,835 + +0.4 4,222,882 + +0.2 4,222,987 + -1.4 4,222,988 - +0.2 4,222,988 - -0.9 4,222,988 - -0.3 4,222,991 + -0.4 4,222,991 + +0.3
Or see this region's nucleotide sequence