Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19375 and PS417_19380 are separated by 104 nucleotides PS417_19380 and PS417_19385 are separated by 10 nucleotides
PS417_19375: PS417_19375 - LysR family transcriptional regulator, at 4,199,066 to 4,199,962
_19375
PS417_19380: PS417_19380 - glutathione S-transferase, at 4,200,067 to 4,200,669
_19380
PS417_19385: PS417_19385 - GntR family transcriptional regulator, at 4,200,680 to 4,201,846
_19385
Position (kb)
4200
4201 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4199.401 kb on - strand, within PS417_19375 at 4199.401 kb on - strand, within PS417_19375 at 4199.738 kb on + strand, within PS417_19375 at 4199.738 kb on + strand, within PS417_19375 at 4199.738 kb on + strand, within PS417_19375 at 4199.738 kb on + strand, within PS417_19375 at 4199.738 kb on + strand, within PS417_19375 at 4199.739 kb on - strand, within PS417_19375 at 4199.739 kb on - strand, within PS417_19375 at 4199.739 kb on - strand, within PS417_19375 at 4199.739 kb on - strand, within PS417_19375 at 4199.739 kb on - strand, within PS417_19375 at 4199.960 kb on + strand at 4199.961 kb on - strand at 4200.086 kb on + strand at 4200.087 kb on - strand at 4200.087 kb on - strand at 4200.087 kb on - strand at 4200.087 kb on - strand at 4200.176 kb on + strand, within PS417_19380 at 4200.177 kb on - strand at 4200.182 kb on + strand, within PS417_19380 at 4200.182 kb on + strand, within PS417_19380 at 4200.182 kb on + strand, within PS417_19380 at 4200.182 kb on + strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.183 kb on - strand, within PS417_19380 at 4200.224 kb on + strand, within PS417_19380 at 4200.225 kb on - strand, within PS417_19380 at 4200.294 kb on - strand, within PS417_19380 at 4200.294 kb on - strand, within PS417_19380 at 4200.294 kb on - strand at 4200.356 kb on + strand, within PS417_19380 at 4200.357 kb on - strand, within PS417_19380 at 4200.357 kb on - strand, within PS417_19380 at 4200.357 kb on - strand, within PS417_19380 at 4200.371 kb on + strand, within PS417_19380 at 4200.629 kb on + strand at 4200.630 kb on - strand at 4200.647 kb on + strand at 4200.657 kb on - strand at 4200.657 kb on - strand at 4200.665 kb on + strand at 4200.665 kb on + strand at 4200.666 kb on - strand at 4200.908 kb on + strand, within PS417_19385 at 4200.909 kb on - strand, within PS417_19385 at 4201.026 kb on + strand, within PS417_19385 at 4201.026 kb on + strand, within PS417_19385 at 4201.191 kb on + strand, within PS417_19385 at 4201.378 kb on - strand, within PS417_19385 at 4201.452 kb on - strand, within PS417_19385 at 4201.452 kb on - strand, within PS417_19385 at 4201.503 kb on + strand, within PS417_19385 at 4201.504 kb on - strand, within PS417_19385 at 4201.545 kb on + strand, within PS417_19385 at 4201.546 kb on - strand, within PS417_19385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,199,401 - PS417_19375 0.37 +0.1 4,199,401 - PS417_19375 0.37 -1.1 4,199,738 + PS417_19375 0.75 -0.3 4,199,738 + PS417_19375 0.75 +0.7 4,199,738 + PS417_19375 0.75 -0.2 4,199,738 + PS417_19375 0.75 -0.7 4,199,738 + PS417_19375 0.75 +0.5 4,199,739 - PS417_19375 0.75 +0.5 4,199,739 - PS417_19375 0.75 -0.5 4,199,739 - PS417_19375 0.75 -0.4 4,199,739 - PS417_19375 0.75 +0.8 4,199,739 - PS417_19375 0.75 -0.6 4,199,960 + +1.6 4,199,961 - -0.4 4,200,086 + -1.4 4,200,087 - -2.3 4,200,087 - -0.1 4,200,087 - -0.0 4,200,087 - -0.7 4,200,176 + PS417_19380 0.18 -2.8 4,200,177 - +0.6 4,200,182 + PS417_19380 0.19 +0.5 4,200,182 + PS417_19380 0.19 -0.2 4,200,182 + PS417_19380 0.19 +0.0 4,200,182 + PS417_19380 0.19 +0.2 4,200,183 - PS417_19380 0.19 -2.4 4,200,183 - PS417_19380 0.19 +1.4 4,200,183 - PS417_19380 0.19 -1.7 4,200,183 - PS417_19380 0.19 -1.0 4,200,183 - PS417_19380 0.19 -0.9 4,200,183 - PS417_19380 0.19 -1.7 4,200,183 - PS417_19380 0.19 -0.8 4,200,183 - PS417_19380 0.19 -0.1 4,200,183 - PS417_19380 0.19 +1.0 4,200,183 - PS417_19380 0.19 +0.3 4,200,183 - PS417_19380 0.19 -1.2 4,200,224 + PS417_19380 0.26 +0.9 4,200,225 - PS417_19380 0.26 -1.2 4,200,294 - PS417_19380 0.38 -0.8 4,200,294 - PS417_19380 0.38 +0.0 4,200,294 - +1.6 4,200,356 + PS417_19380 0.48 +0.4 4,200,357 - PS417_19380 0.48 +0.5 4,200,357 - PS417_19380 0.48 -0.6 4,200,357 - PS417_19380 0.48 -1.9 4,200,371 + PS417_19380 0.50 -0.4 4,200,629 + -0.4 4,200,630 - -0.7 4,200,647 + -0.7 4,200,657 - +1.0 4,200,657 - +0.2 4,200,665 + +2.5 4,200,665 + -0.7 4,200,666 - -0.8 4,200,908 + PS417_19385 0.20 -0.2 4,200,909 - PS417_19385 0.20 -0.1 4,201,026 + PS417_19385 0.30 -0.1 4,201,026 + PS417_19385 0.30 +1.9 4,201,191 + PS417_19385 0.44 -0.9 4,201,378 - PS417_19385 0.60 -0.0 4,201,452 - PS417_19385 0.66 -1.0 4,201,452 - PS417_19385 0.66 -0.2 4,201,503 + PS417_19385 0.71 -1.3 4,201,504 - PS417_19385 0.71 +0.2 4,201,545 + PS417_19385 0.74 +0.0 4,201,546 - PS417_19385 0.74 -1.2
Or see this region's nucleotide sequence