Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01640 and PS417_01645 are separated by 287 nucleotides PS417_01645 and PS417_01650 are separated by 77 nucleotides PS417_01650 and PS417_01655 are separated by 150 nucleotides
PS417_01640: PS417_01640 - histidine kinase, at 359,602 to 360,687
_01640
PS417_01645: PS417_01645 - cupin, at 360,975 to 361,400
_01645
PS417_01650: PS417_01650 - chorismate mutase, at 361,478 to 362,014
_01650
PS417_01655: PS417_01655 - glutamine synthetase, at 362,165 to 363,571
_01655
Position (kb) 361
362
363 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 360.599 kb on + strand at 360.599 kb on + strand at 360.600 kb on - strand at 360.600 kb on - strand at 360.600 kb on - strand at 360.626 kb on - strand at 360.733 kb on + strand at 360.734 kb on - strand at 360.895 kb on + strand at 360.896 kb on - strand at 360.896 kb on - strand at 360.897 kb on + strand at 361.159 kb on + strand, within PS417_01645 at 361.159 kb on + strand, within PS417_01645 at 361.160 kb on - strand, within PS417_01645 at 361.224 kb on + strand, within PS417_01645 at 361.290 kb on - strand, within PS417_01645 at 361.483 kb on + strand at 361.483 kb on + strand at 361.484 kb on - strand at 361.606 kb on + strand, within PS417_01650 at 361.662 kb on - strand, within PS417_01650 at 361.734 kb on + strand, within PS417_01650 at 361.734 kb on + strand, within PS417_01650 at 361.734 kb on + strand, within PS417_01650 at 361.735 kb on - strand, within PS417_01650 at 361.803 kb on + strand, within PS417_01650 at 361.803 kb on + strand, within PS417_01650 at 361.804 kb on - strand, within PS417_01650 at 361.804 kb on - strand, within PS417_01650 at 362.068 kb on + strand at 362.068 kb on + strand at 362.069 kb on - strand at 362.069 kb on - strand at 362.069 kb on - strand at 362.069 kb on - strand at 362.143 kb on + strand at 362.143 kb on + strand at 362.143 kb on + strand at 362.149 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 360,599 + -0.9 360,599 + -1.6 360,600 - +1.1 360,600 - +0.8 360,600 - +0.3 360,626 - +0.5 360,733 + +0.4 360,734 - +1.6 360,895 + +1.6 360,896 - +1.1 360,896 - +0.5 360,897 + +0.1 361,159 + PS417_01645 0.43 +0.6 361,159 + PS417_01645 0.43 -2.8 361,160 - PS417_01645 0.43 +0.4 361,224 + PS417_01645 0.58 -1.0 361,290 - PS417_01645 0.74 -0.9 361,483 + -0.1 361,483 + -2.2 361,484 - +0.0 361,606 + PS417_01650 0.24 -3.7 361,662 - PS417_01650 0.34 +2.7 361,734 + PS417_01650 0.48 +1.0 361,734 + PS417_01650 0.48 +0.1 361,734 + PS417_01650 0.48 +0.1 361,735 - PS417_01650 0.48 -0.2 361,803 + PS417_01650 0.61 +0.4 361,803 + PS417_01650 0.61 -0.1 361,804 - PS417_01650 0.61 +0.5 361,804 - PS417_01650 0.61 +1.7 362,068 + -0.2 362,068 + +0.4 362,069 - -0.0 362,069 - +2.6 362,069 - +0.4 362,069 - -0.1 362,143 + +1.2 362,143 + +0.0 362,143 + +0.9 362,149 - +0.9
Or see this region's nucleotide sequence