Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15500 and PS417_15505 are separated by 134 nucleotides PS417_15505 and PS417_15510 are separated by 55 nucleotides PS417_15510 and PS417_15515 are separated by 90 nucleotides PS417_15515 and PS417_15520 are separated by 23 nucleotides
PS417_15500: PS417_15500 - membrane protein, at 3,395,563 to 3,396,330
_15500
PS417_15505: PS417_15505 - glutaminase, at 3,396,465 to 3,397,373
_15505
PS417_15510: PS417_15510 - hypothetical protein, at 3,397,429 to 3,397,773
_15510
PS417_15515: PS417_15515 - hypothetical protein, at 3,397,864 to 3,398,211
_15515
PS417_15520: PS417_15520 - hypothetical protein, at 3,398,235 to 3,398,645
_15520
Position (kb)
3396
3397
3398 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3395.539 kb on + strand at 3395.540 kb on - strand at 3395.617 kb on + strand at 3395.632 kb on + strand at 3395.632 kb on + strand at 3395.718 kb on + strand, within PS417_15500 at 3395.774 kb on + strand, within PS417_15500 at 3395.854 kb on + strand, within PS417_15500 at 3395.854 kb on + strand, within PS417_15500 at 3395.854 kb on + strand, within PS417_15500 at 3395.855 kb on - strand at 3395.855 kb on - strand, within PS417_15500 at 3395.855 kb on - strand, within PS417_15500 at 3395.855 kb on - strand, within PS417_15500 at 3396.104 kb on - strand, within PS417_15500 at 3396.104 kb on - strand, within PS417_15500 at 3396.106 kb on - strand, within PS417_15500 at 3396.106 kb on - strand, within PS417_15500 at 3396.183 kb on - strand, within PS417_15500 at 3396.183 kb on - strand, within PS417_15500 at 3396.226 kb on + strand, within PS417_15500 at 3396.226 kb on + strand, within PS417_15500 at 3396.226 kb on + strand, within PS417_15500 at 3396.227 kb on - strand, within PS417_15500 at 3396.342 kb on + strand at 3396.427 kb on + strand at 3396.428 kb on - strand at 3396.428 kb on - strand at 3396.569 kb on + strand, within PS417_15505 at 3396.569 kb on + strand, within PS417_15505 at 3396.662 kb on - strand, within PS417_15505 at 3396.818 kb on - strand, within PS417_15505 at 3396.818 kb on - strand, within PS417_15505 at 3397.303 kb on + strand at 3397.303 kb on + strand at 3397.425 kb on + strand at 3397.425 kb on + strand at 3397.426 kb on - strand at 3397.502 kb on - strand, within PS417_15510 at 3397.656 kb on + strand, within PS417_15510 at 3397.751 kb on + strand at 3397.752 kb on - strand at 3397.752 kb on - strand at 3397.810 kb on + strand at 3398.227 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,395,539 + +0.8 3,395,540 - -0.5 3,395,617 + -1.2 3,395,632 + -0.3 3,395,632 + +0.5 3,395,718 + PS417_15500 0.20 +0.2 3,395,774 + PS417_15500 0.27 -1.1 3,395,854 + PS417_15500 0.38 -0.3 3,395,854 + PS417_15500 0.38 +0.3 3,395,854 + PS417_15500 0.38 +1.2 3,395,855 - +2.4 3,395,855 - PS417_15500 0.38 -0.3 3,395,855 - PS417_15500 0.38 +0.4 3,395,855 - PS417_15500 0.38 -0.8 3,396,104 - PS417_15500 0.70 +0.4 3,396,104 - PS417_15500 0.70 -0.6 3,396,106 - PS417_15500 0.71 +2.2 3,396,106 - PS417_15500 0.71 -0.7 3,396,183 - PS417_15500 0.81 -0.5 3,396,183 - PS417_15500 0.81 -0.1 3,396,226 + PS417_15500 0.86 +2.8 3,396,226 + PS417_15500 0.86 +0.9 3,396,226 + PS417_15500 0.86 -0.5 3,396,227 - PS417_15500 0.86 -0.2 3,396,342 + -0.2 3,396,427 + -0.3 3,396,428 - -0.7 3,396,428 - -0.2 3,396,569 + PS417_15505 0.11 -1.3 3,396,569 + PS417_15505 0.11 -0.7 3,396,662 - PS417_15505 0.22 -0.0 3,396,818 - PS417_15505 0.39 -1.2 3,396,818 - PS417_15505 0.39 -0.8 3,397,303 + +0.1 3,397,303 + -0.2 3,397,425 + -1.1 3,397,425 + +1.4 3,397,426 - +1.4 3,397,502 - PS417_15510 0.21 +0.7 3,397,656 + PS417_15510 0.66 +0.9 3,397,751 + +0.9 3,397,752 - +2.3 3,397,752 - +1.8 3,397,810 + -0.3 3,398,227 - +1.2
Or see this region's nucleotide sequence