Strain Fitness in Pseudomonas simiae WCS417 around PS417_15025

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_15020 and PS417_15025 are separated by 129 nucleotidesPS417_15025 and PS417_15030 are separated by 9 nucleotidesPS417_15030 and PS417_15035 are separated by 0 nucleotides PS417_15020: PS417_15020 - 2-hydroxyacid dehydrogenase, at 3,283,438 to 3,284,391 _15020 PS417_15025: PS417_15025 - aminoglycoside phosphotransferase, at 3,284,521 to 3,285,588 _15025 PS417_15030: PS417_15030 - short-chain dehydrogenase, at 3,285,598 to 3,286,365 _15030 PS417_15035: PS417_15035 - alkylhydroperoxidase, at 3,286,366 to 3,286,809 _15035 Position (kb) 3284 3285 3286Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3283.609 kb on + strand, within PS417_15020at 3283.680 kb on - strand, within PS417_15020at 3283.680 kb on - strand, within PS417_15020at 3283.945 kb on + strand, within PS417_15020at 3283.978 kb on + strand, within PS417_15020at 3283.978 kb on + strand, within PS417_15020at 3284.136 kb on - strand, within PS417_15020at 3284.335 kb on + strandat 3284.335 kb on + strandat 3284.336 kb on - strandat 3284.336 kb on - strandat 3284.336 kb on - strandat 3284.430 kb on + strandat 3284.430 kb on + strandat 3284.430 kb on + strandat 3284.431 kb on - strandat 3284.431 kb on - strandat 3284.433 kb on + strandat 3284.433 kb on + strandat 3284.434 kb on - strandat 3284.472 kb on + strandat 3284.590 kb on + strandat 3284.668 kb on + strand, within PS417_15025at 3284.668 kb on + strand, within PS417_15025at 3284.668 kb on + strand, within PS417_15025at 3284.669 kb on - strand, within PS417_15025at 3284.669 kb on - strand, within PS417_15025at 3284.809 kb on + strand, within PS417_15025at 3284.810 kb on - strand, within PS417_15025at 3284.851 kb on + strandat 3285.104 kb on + strand, within PS417_15025at 3285.324 kb on + strand, within PS417_15025at 3285.324 kb on + strand, within PS417_15025at 3285.325 kb on - strand, within PS417_15025at 3285.382 kb on + strand, within PS417_15025at 3285.427 kb on + strand, within PS417_15025at 3285.430 kb on + strand, within PS417_15025at 3285.430 kb on + strand, within PS417_15025at 3285.430 kb on + strand, within PS417_15025at 3285.436 kb on + strand, within PS417_15025at 3285.436 kb on + strand, within PS417_15025at 3285.436 kb on + strandat 3285.437 kb on - strand, within PS417_15025at 3285.586 kb on + strandat 3285.597 kb on + strandat 3285.597 kb on + strandat 3285.598 kb on - strandat 3285.967 kb on + strand, within PS417_15030at 3286.318 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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3,283,609 + PS417_15020 0.18 -0.2
3,283,680 - PS417_15020 0.25 -0.1
3,283,680 - PS417_15020 0.25 -0.4
3,283,945 + PS417_15020 0.53 +0.9
3,283,978 + PS417_15020 0.57 +0.5
3,283,978 + PS417_15020 0.57 -0.3
3,284,136 - PS417_15020 0.73 +0.6
3,284,335 + -0.3
3,284,335 + -0.6
3,284,336 - -0.3
3,284,336 - +0.7
3,284,336 - -0.7
3,284,430 + -0.9
3,284,430 + +0.4
3,284,430 + +0.3
3,284,431 - -1.9
3,284,431 - -0.8
3,284,433 + -1.2
3,284,433 + +0.3
3,284,434 - +0.2
3,284,472 + -0.2
3,284,590 + +0.3
3,284,668 + PS417_15025 0.14 -0.1
3,284,668 + PS417_15025 0.14 +0.8
3,284,668 + PS417_15025 0.14 -0.2
3,284,669 - PS417_15025 0.14 -0.1
3,284,669 - PS417_15025 0.14 +1.0
3,284,809 + PS417_15025 0.27 +1.4
3,284,810 - PS417_15025 0.27 +0.2
3,284,851 + -0.8
3,285,104 + PS417_15025 0.55 +1.2
3,285,324 + PS417_15025 0.75 -0.3
3,285,324 + PS417_15025 0.75 +0.2
3,285,325 - PS417_15025 0.75 +0.9
3,285,382 + PS417_15025 0.81 +0.4
3,285,427 + PS417_15025 0.85 -1.4
3,285,430 + PS417_15025 0.85 +3.1
3,285,430 + PS417_15025 0.85 +0.4
3,285,430 + PS417_15025 0.85 +0.3
3,285,436 + PS417_15025 0.86 +0.2
3,285,436 + PS417_15025 0.86 -0.5
3,285,436 + +0.2
3,285,437 - PS417_15025 0.86 -0.9
3,285,586 + +1.4
3,285,597 + +1.2
3,285,597 + +0.0
3,285,598 - -0.1
3,285,967 + PS417_15030 0.48 +0.3
3,286,318 + -0.1

Or see this region's nucleotide sequence