Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14815 and PS417_14825 are separated by 84 nucleotides PS417_14825 and PS417_14830 are separated by 83 nucleotides PS417_14830 and PS417_14835 are separated by 53 nucleotides
PS417_14815: PS417_14815 - diguanylate cyclase, at 3,238,583 to 3,239,749
_14815
PS417_14825: PS417_14825 - hypothetical protein, at 3,239,834 to 3,240,139
_14825
PS417_14830: PS417_14830 - Cro/Cl family transcriptional regulator, at 3,240,223 to 3,241,011
_14830
PS417_14835: PS417_14835 - amidase, at 3,241,065 to 3,242,774
_14835
Position (kb)
3240
3241
3242 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3239.264 kb on + strand, within PS417_14815 at 3239.707 kb on + strand at 3239.707 kb on + strand at 3239.742 kb on - strand at 3239.747 kb on + strand at 3239.966 kb on + strand at 3239.966 kb on + strand, within PS417_14825 at 3239.992 kb on - strand, within PS417_14825 at 3240.091 kb on - strand, within PS417_14825 at 3240.126 kb on + strand at 3240.126 kb on + strand at 3240.127 kb on - strand at 3240.178 kb on + strand at 3240.237 kb on + strand at 3240.455 kb on - strand, within PS417_14830 at 3240.515 kb on + strand, within PS417_14830 at 3240.515 kb on + strand, within PS417_14830 at 3240.516 kb on - strand, within PS417_14830 at 3240.516 kb on - strand, within PS417_14830 at 3240.599 kb on + strand, within PS417_14830 at 3240.600 kb on - strand, within PS417_14830 at 3241.014 kb on + strand at 3241.020 kb on + strand at 3241.135 kb on + strand at 3241.659 kb on + strand, within PS417_14835 at 3241.932 kb on + strand, within PS417_14835 at 3241.933 kb on - strand, within PS417_14835
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,239,264 + PS417_14815 0.58 +0.2 3,239,707 + -0.6 3,239,707 + +0.2 3,239,742 - -0.5 3,239,747 + -0.3 3,239,966 + +0.2 3,239,966 + PS417_14825 0.43 -0.2 3,239,992 - PS417_14825 0.52 +0.3 3,240,091 - PS417_14825 0.84 +1.8 3,240,126 + +0.3 3,240,126 + +0.5 3,240,127 - -1.8 3,240,178 + +0.7 3,240,237 + -0.0 3,240,455 - PS417_14830 0.29 -0.6 3,240,515 + PS417_14830 0.37 -0.8 3,240,515 + PS417_14830 0.37 -0.5 3,240,516 - PS417_14830 0.37 -1.0 3,240,516 - PS417_14830 0.37 -0.2 3,240,599 + PS417_14830 0.48 -0.7 3,240,600 - PS417_14830 0.48 +0.1 3,241,014 + -0.3 3,241,020 + +0.2 3,241,135 + -1.1 3,241,659 + PS417_14835 0.35 -0.5 3,241,932 + PS417_14835 0.51 -0.7 3,241,933 - PS417_14835 0.51 -0.8
Or see this region's nucleotide sequence