Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13205 and PS417_13210 overlap by 4 nucleotides PS417_13210 and PS417_13215 overlap by 4 nucleotides PS417_13215 and PS417_13220 overlap by 8 nucleotides PS417_13220 and PS417_13225 overlap by 4 nucleotides
PS417_13205: PS417_13205 - isochorismate-pyruvate lyase, at 2,888,360 to 2,888,668
_13205
PS417_13210: PS417_13210 - enantio-pyochelin biosynthetic protein PchC, at 2,888,665 to 2,889,423
_13210
PS417_13215: PS417_13215 - Triostin synthetase I, at 2,889,420 to 2,891,018
_13215
PS417_13220: PS417_13220 - hypothetical protein, at 2,891,011 to 2,891,730
_13220
PS417_13225: PS417_13225 - thiazolinyl imide reductase, at 2,891,727 to 2,892,872
_13225
Position (kb)
2889
2890
2891
2892 Strain fitness (log2 ratio)
-1
0
1 at 2888.569 kb on - strand, within PS417_13205 at 2888.569 kb on - strand, within PS417_13205 at 2889.645 kb on + strand, within PS417_13215 at 2889.645 kb on + strand, within PS417_13215 at 2889.645 kb on + strand, within PS417_13215 at 2889.841 kb on + strand, within PS417_13215 at 2889.841 kb on + strand, within PS417_13215 at 2889.842 kb on - strand at 2889.860 kb on - strand, within PS417_13215 at 2889.860 kb on - strand, within PS417_13215 at 2889.860 kb on - strand, within PS417_13215 at 2890.399 kb on + strand, within PS417_13215 at 2890.642 kb on + strand, within PS417_13215 at 2890.643 kb on - strand, within PS417_13215 at 2890.643 kb on - strand at 2891.003 kb on + strand at 2891.108 kb on - strand, within PS417_13220 at 2891.192 kb on - strand at 2891.782 kb on - strand at 2892.010 kb on + strand, within PS417_13225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,888,569 - PS417_13205 0.68 -1.3 2,888,569 - PS417_13205 0.68 -0.5 2,889,645 + PS417_13215 0.14 +0.2 2,889,645 + PS417_13215 0.14 -0.0 2,889,645 + PS417_13215 0.14 +0.6 2,889,841 + PS417_13215 0.26 +0.9 2,889,841 + PS417_13215 0.26 -0.3 2,889,842 - -1.7 2,889,860 - PS417_13215 0.28 +0.7 2,889,860 - PS417_13215 0.28 +0.2 2,889,860 - PS417_13215 0.28 +0.7 2,890,399 + PS417_13215 0.61 +0.2 2,890,642 + PS417_13215 0.76 +0.5 2,890,643 - PS417_13215 0.76 +1.0 2,890,643 - -1.4 2,891,003 + -0.4 2,891,108 - PS417_13220 0.13 +1.0 2,891,192 - -1.0 2,891,782 - -0.3 2,892,010 + PS417_13225 0.25 +1.4
Or see this region's nucleotide sequence