Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01195 and PS417_01200 are separated by 39 nucleotides PS417_01200 and PS417_01205 are separated by 104 nucleotides PS417_01205 and PS417_01210 are separated by 179 nucleotides
PS417_01195: PS417_01195 - RNA-binding protein S4, at 262,999 to 263,406
_01195
PS417_01200: PS417_01200 - phosphoesterase, at 263,446 to 264,246
_01200
PS417_01205: PS417_01205 - molecular chaperone Hsp33, at 264,351 to 265,253
_01205
PS417_01210: PS417_01210 - hypothetical protein, at 265,433 to 266,974
_01210
Position (kb)
264
265
266 Strain fitness (log2 ratio)
-1
0
1
2
3 at 263.539 kb on + strand, within PS417_01200 at 263.561 kb on + strand, within PS417_01200 at 263.561 kb on + strand, within PS417_01200 at 263.562 kb on - strand, within PS417_01200 at 263.562 kb on - strand, within PS417_01200 at 263.609 kb on + strand, within PS417_01200 at 263.609 kb on + strand, within PS417_01200 at 263.609 kb on + strand, within PS417_01200 at 263.859 kb on + strand, within PS417_01200 at 263.877 kb on + strand, within PS417_01200 at 263.878 kb on - strand, within PS417_01200 at 263.937 kb on + strand, within PS417_01200 at 263.944 kb on + strand, within PS417_01200 at 263.945 kb on - strand, within PS417_01200 at 264.058 kb on + strand, within PS417_01200 at 264.184 kb on + strand at 264.184 kb on + strand at 264.246 kb on + strand at 264.247 kb on - strand at 264.316 kb on + strand at 264.316 kb on + strand at 265.370 kb on + strand at 265.371 kb on - strand at 265.455 kb on + strand at 265.457 kb on + strand at 265.600 kb on + strand, within PS417_01210 at 265.600 kb on + strand, within PS417_01210 at 265.601 kb on - strand, within PS417_01210 at 265.601 kb on - strand, within PS417_01210 at 265.601 kb on - strand, within PS417_01210 at 265.601 kb on - strand, within PS417_01210 at 265.601 kb on - strand, within PS417_01210 at 265.826 kb on + strand, within PS417_01210 at 265.826 kb on + strand, within PS417_01210 at 266.204 kb on + strand, within PS417_01210 at 266.204 kb on + strand, within PS417_01210 at 266.205 kb on - strand, within PS417_01210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 263,539 + PS417_01200 0.12 +1.6 263,561 + PS417_01200 0.14 +2.1 263,561 + PS417_01200 0.14 +2.9 263,562 - PS417_01200 0.14 +2.2 263,562 - PS417_01200 0.14 +1.5 263,609 + PS417_01200 0.20 +3.2 263,609 + PS417_01200 0.20 +1.2 263,609 + PS417_01200 0.20 +0.7 263,859 + PS417_01200 0.52 +2.0 263,877 + PS417_01200 0.54 +2.2 263,878 - PS417_01200 0.54 -1.6 263,937 + PS417_01200 0.61 +2.0 263,944 + PS417_01200 0.62 +2.6 263,945 - PS417_01200 0.62 +1.7 264,058 + PS417_01200 0.76 +0.2 264,184 + +2.6 264,184 + +2.0 264,246 + +1.4 264,247 - +2.9 264,316 + +0.3 264,316 + +1.5 265,370 + +0.5 265,371 - -0.1 265,455 + -0.6 265,457 + -1.3 265,600 + PS417_01210 0.11 +0.2 265,600 + PS417_01210 0.11 -1.2 265,601 - PS417_01210 0.11 +0.8 265,601 - PS417_01210 0.11 -0.2 265,601 - PS417_01210 0.11 +0.2 265,601 - PS417_01210 0.11 +0.3 265,601 - PS417_01210 0.11 -0.9 265,826 + PS417_01210 0.25 +0.3 265,826 + PS417_01210 0.25 -1.0 266,204 + PS417_01210 0.50 +1.2 266,204 + PS417_01210 0.50 -0.8 266,205 - PS417_01210 0.50 +0.4
Or see this region's nucleotide sequence