Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11285 and PS417_11290 overlap by 8 nucleotides PS417_11290 and PS417_11295 overlap by 4 nucleotides PS417_11295 and PS417_11300 are separated by 352 nucleotides
PS417_11285: PS417_11285 - general secretion pathway protein GspL, at 2,415,251 to 2,416,312
_11285
PS417_11290: PS417_11290 - general secretion pathway protein GspM, at 2,416,305 to 2,416,817
_11290
PS417_11295: PS417_11295 - general secretion pathway protein GspD, at 2,416,814 to 2,419,108
_11295
PS417_11300: PS417_11300 - general secretion pathway protein GspE, at 2,419,461 to 2,420,879
_11300
Position (kb)
2416
2417
2418
2419
2420 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2416.294 kb on - strand at 2416.484 kb on - strand, within PS417_11290 at 2416.949 kb on - strand at 2417.082 kb on + strand, within PS417_11295 at 2417.226 kb on - strand, within PS417_11295 at 2417.369 kb on - strand, within PS417_11295 at 2417.784 kb on - strand, within PS417_11295 at 2417.784 kb on - strand, within PS417_11295 at 2417.920 kb on + strand, within PS417_11295 at 2417.921 kb on - strand, within PS417_11295 at 2418.739 kb on + strand, within PS417_11295 at 2418.739 kb on + strand, within PS417_11295 at 2418.911 kb on + strand at 2418.912 kb on - strand at 2418.943 kb on + strand at 2418.944 kb on - strand at 2418.967 kb on + strand at 2419.003 kb on + strand at 2419.121 kb on + strand at 2419.121 kb on + strand at 2419.122 kb on - strand at 2419.122 kb on - strand at 2419.122 kb on - strand at 2419.361 kb on + strand at 2419.436 kb on + strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.468 kb on + strand at 2419.469 kb on - strand at 2419.531 kb on + strand at 2419.703 kb on - strand, within PS417_11300 at 2419.703 kb on - strand, within PS417_11300 at 2419.728 kb on - strand, within PS417_11300 at 2419.728 kb on - strand, within PS417_11300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,416,294 - -0.6 2,416,484 - PS417_11290 0.35 +0.1 2,416,949 - -0.3 2,417,082 + PS417_11295 0.12 -0.9 2,417,226 - PS417_11295 0.18 -1.0 2,417,369 - PS417_11295 0.24 -0.2 2,417,784 - PS417_11295 0.42 -1.9 2,417,784 - PS417_11295 0.42 -0.1 2,417,920 + PS417_11295 0.48 -0.6 2,417,921 - PS417_11295 0.48 -1.6 2,418,739 + PS417_11295 0.84 -0.3 2,418,739 + PS417_11295 0.84 -1.1 2,418,911 + +0.1 2,418,912 - +0.2 2,418,943 + -1.5 2,418,944 - -0.2 2,418,967 + -0.2 2,419,003 + -0.3 2,419,121 + -1.4 2,419,121 + +1.7 2,419,122 - -0.2 2,419,122 - +0.1 2,419,122 - -0.8 2,419,361 + +0.1 2,419,436 + +2.4 2,419,437 - +0.6 2,419,437 - +0.5 2,419,437 - -0.3 2,419,437 - +0.4 2,419,437 - +0.3 2,419,437 - -0.8 2,419,468 + +1.1 2,419,469 - +0.2 2,419,531 + -0.3 2,419,703 - PS417_11300 0.17 +0.7 2,419,703 - PS417_11300 0.17 -0.3 2,419,728 - PS417_11300 0.19 -0.9 2,419,728 - PS417_11300 0.19 +0.6
Or see this region's nucleotide sequence