Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09230 and PS417_09235 are separated by 112 nucleotides PS417_09235 and PS417_09240 are separated by 105 nucleotides PS417_09240 and PS417_09245 are separated by 172 nucleotides
PS417_09230: PS417_09230 - glycerol acyltransferase, at 2,004,802 to 2,006,676
_09230
PS417_09235: PS417_09235 - molecular chaperone, at 2,006,789 to 2,007,103
_09235
PS417_09240: PS417_09240 - sodium transporter, at 2,007,209 to 2,008,174
_09240
PS417_09245: PS417_09245 - recombinase RdgC, at 2,008,347 to 2,009,267
_09245
Position (kb)
2007
2008
2009 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2006.254 kb on + strand, within PS417_09230 at 2006.255 kb on - strand, within PS417_09230 at 2006.255 kb on - strand, within PS417_09230 at 2006.255 kb on - strand, within PS417_09230 at 2006.257 kb on + strand, within PS417_09230 at 2006.257 kb on + strand, within PS417_09230 at 2006.258 kb on - strand, within PS417_09230 at 2006.258 kb on - strand, within PS417_09230 at 2006.347 kb on + strand, within PS417_09230 at 2006.347 kb on + strand, within PS417_09230 at 2006.674 kb on + strand at 2006.675 kb on - strand at 2006.740 kb on + strand at 2007.039 kb on + strand, within PS417_09235 at 2007.114 kb on + strand at 2007.141 kb on + strand at 2007.270 kb on + strand at 2007.271 kb on - strand at 2008.135 kb on + strand at 2008.135 kb on + strand at 2008.136 kb on - strand at 2008.284 kb on - strand at 2008.348 kb on + strand at 2008.348 kb on + strand at 2008.970 kb on - strand at 2009.049 kb on - strand, within PS417_09245 at 2009.049 kb on - strand, within PS417_09245 at 2009.144 kb on - strand, within PS417_09245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,006,254 + PS417_09230 0.77 +0.9 2,006,255 - PS417_09230 0.77 -0.9 2,006,255 - PS417_09230 0.77 +2.3 2,006,255 - PS417_09230 0.77 -0.0 2,006,257 + PS417_09230 0.78 +0.3 2,006,257 + PS417_09230 0.78 +0.5 2,006,258 - PS417_09230 0.78 +0.2 2,006,258 - PS417_09230 0.78 -1.2 2,006,347 + PS417_09230 0.82 -0.5 2,006,347 + PS417_09230 0.82 +0.4 2,006,674 + -0.3 2,006,675 - -0.2 2,006,740 + -0.2 2,007,039 + PS417_09235 0.79 -3.8 2,007,114 + +0.8 2,007,141 + +0.1 2,007,270 + -1.1 2,007,271 - -1.4 2,008,135 + -0.1 2,008,135 + +0.0 2,008,136 - +1.7 2,008,284 - -0.5 2,008,348 + -0.1 2,008,348 + +0.2 2,008,970 - +2.7 2,009,049 - PS417_09245 0.76 +0.5 2,009,049 - PS417_09245 0.76 +0.8 2,009,144 - PS417_09245 0.87 -1.8
Or see this region's nucleotide sequence