Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07430 and PS417_07435 are separated by 78 nucleotides PS417_07435 and PS417_07440 overlap by 4 nucleotides PS417_07440 and PS417_07445 are separated by 68 nucleotides PS417_07445 and PS417_07450 are separated by 25 nucleotides PS417_07450 and PS417_07455 are separated by 30 nucleotides
PS417_07430: PS417_07430 - hypothetical protein, at 1,635,960 to 1,636,646
_07430
PS417_07435: PS417_07435 - hypothetical protein, at 1,636,725 to 1,637,000
_07435
PS417_07440: PS417_07440 - cyclic nucleotide-binding protein, at 1,636,997 to 1,637,482
_07440
PS417_07445: PS417_07445 - peptidase S9, at 1,637,551 to 1,639,593
_07445
PS417_07450: PS417_07450 - MFS transporter, at 1,639,619 to 1,640,155
_07450
PS417_07455: PS417_07455 - glutamine amidotransferase, at 1,640,186 to 1,640,962
_07455
Position (kb)
1637
1638
1639
1640 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1636.687 kb on + strand at 1636.687 kb on + strand at 1636.741 kb on + strand at 1636.999 kb on - strand at 1637.016 kb on - strand at 1637.056 kb on + strand, within PS417_07440 at 1637.061 kb on - strand, within PS417_07440 at 1637.192 kb on + strand, within PS417_07440 at 1637.368 kb on - strand, within PS417_07440 at 1637.450 kb on + strand at 1637.451 kb on - strand at 1637.543 kb on + strand at 1637.552 kb on + strand at 1637.552 kb on + strand at 1637.553 kb on - strand at 1637.596 kb on + strand at 1637.596 kb on + strand at 1637.596 kb on + strand at 1637.596 kb on + strand at 1637.596 kb on + strand at 1637.597 kb on - strand at 1637.597 kb on - strand at 1637.597 kb on - strand at 1637.597 kb on - strand at 1637.605 kb on + strand at 1637.605 kb on + strand at 1637.605 kb on + strand at 1637.748 kb on - strand at 1637.807 kb on + strand, within PS417_07445 at 1637.807 kb on + strand, within PS417_07445 at 1637.808 kb on - strand, within PS417_07445 at 1638.227 kb on + strand at 1638.228 kb on - strand, within PS417_07445 at 1638.249 kb on - strand, within PS417_07445 at 1638.348 kb on - strand, within PS417_07445 at 1638.503 kb on + strand, within PS417_07445 at 1638.504 kb on - strand, within PS417_07445 at 1638.504 kb on - strand, within PS417_07445 at 1638.513 kb on - strand, within PS417_07445 at 1638.723 kb on - strand, within PS417_07445 at 1638.723 kb on - strand, within PS417_07445 at 1638.723 kb on - strand, within PS417_07445 at 1638.794 kb on + strand, within PS417_07445 at 1638.794 kb on + strand, within PS417_07445 at 1638.828 kb on + strand, within PS417_07445 at 1638.828 kb on + strand, within PS417_07445 at 1639.002 kb on - strand, within PS417_07445 at 1639.136 kb on + strand, within PS417_07445 at 1639.145 kb on + strand, within PS417_07445 at 1639.145 kb on + strand, within PS417_07445 at 1639.146 kb on - strand, within PS417_07445 at 1639.146 kb on - strand, within PS417_07445 at 1639.175 kb on + strand, within PS417_07445 at 1639.204 kb on - strand at 1639.358 kb on + strand, within PS417_07445 at 1639.361 kb on + strand, within PS417_07445 at 1639.361 kb on + strand, within PS417_07445 at 1639.361 kb on + strand, within PS417_07445 at 1639.500 kb on - strand at 1639.599 kb on + strand at 1639.660 kb on + strand at 1639.812 kb on + strand, within PS417_07450 at 1639.958 kb on + strand, within PS417_07450 at 1639.958 kb on + strand, within PS417_07450 at 1639.959 kb on - strand, within PS417_07450 at 1640.154 kb on - strand at 1640.154 kb on - strand at 1640.196 kb on + strand at 1640.306 kb on + strand, within PS417_07455 at 1640.307 kb on - strand, within PS417_07455 at 1640.547 kb on - strand, within PS417_07455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,636,687 + -0.8 1,636,687 + -1.4 1,636,741 + +2.7 1,636,999 - +1.0 1,637,016 - -1.5 1,637,056 + PS417_07440 0.12 +0.1 1,637,061 - PS417_07440 0.13 -0.3 1,637,192 + PS417_07440 0.40 +0.8 1,637,368 - PS417_07440 0.76 -1.9 1,637,450 + -0.2 1,637,451 - +0.2 1,637,543 + +0.5 1,637,552 + +0.5 1,637,552 + -0.4 1,637,553 - -0.5 1,637,596 + -0.2 1,637,596 + +0.5 1,637,596 + -0.8 1,637,596 + -0.6 1,637,596 + +0.7 1,637,597 - +0.2 1,637,597 - +0.3 1,637,597 - +0.0 1,637,597 - +0.3 1,637,605 + +1.0 1,637,605 + -0.8 1,637,605 + +0.0 1,637,748 - +0.2 1,637,807 + PS417_07445 0.13 -0.0 1,637,807 + PS417_07445 0.13 +1.6 1,637,808 - PS417_07445 0.13 +1.5 1,638,227 + +0.1 1,638,228 - PS417_07445 0.33 -0.0 1,638,249 - PS417_07445 0.34 +0.2 1,638,348 - PS417_07445 0.39 +0.8 1,638,503 + PS417_07445 0.47 +0.8 1,638,504 - PS417_07445 0.47 +0.6 1,638,504 - PS417_07445 0.47 +0.8 1,638,513 - PS417_07445 0.47 +0.1 1,638,723 - PS417_07445 0.57 -0.1 1,638,723 - PS417_07445 0.57 +0.7 1,638,723 - PS417_07445 0.57 +0.8 1,638,794 + PS417_07445 0.61 +0.3 1,638,794 + PS417_07445 0.61 +1.4 1,638,828 + PS417_07445 0.63 +1.5 1,638,828 + PS417_07445 0.63 +0.4 1,639,002 - PS417_07445 0.71 +0.7 1,639,136 + PS417_07445 0.78 -0.0 1,639,145 + PS417_07445 0.78 +0.4 1,639,145 + PS417_07445 0.78 +0.2 1,639,146 - PS417_07445 0.78 -1.3 1,639,146 - PS417_07445 0.78 +1.7 1,639,175 + PS417_07445 0.79 -0.6 1,639,204 - +1.1 1,639,358 + PS417_07445 0.88 +0.7 1,639,361 + PS417_07445 0.89 +0.5 1,639,361 + PS417_07445 0.89 -0.0 1,639,361 + PS417_07445 0.89 +2.2 1,639,500 - +1.2 1,639,599 + -0.1 1,639,660 + -0.0 1,639,812 + PS417_07450 0.36 +1.4 1,639,958 + PS417_07450 0.63 +0.7 1,639,958 + PS417_07450 0.63 +0.7 1,639,959 - PS417_07450 0.63 -1.6 1,640,154 - -0.4 1,640,154 - +1.2 1,640,196 + -0.3 1,640,306 + PS417_07455 0.15 +1.3 1,640,307 - PS417_07455 0.16 +0.4 1,640,547 - PS417_07455 0.46 -0.2
Or see this region's nucleotide sequence