Experiment: Temperature shift 30_to_4; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1241 and PP_1242 are separated by 356 nucleotides PP_1242 and PP_1244 are separated by 135 nucleotides PP_1244 and PP_1245 are separated by 508 nucleotides
PP_1241: PP_1241 - conserved protein of unknown function, at 1,418,532 to 1,418,882
_1241
PP_1242: PP_1242 - conserved protein of unknown function, at 1,419,239 to 1,419,511
_1242
PP_1244: PP_1244 - conserved protein of unknown function, at 1,419,647 to 1,420,012
_1244
PP_1245: PP_1245 - conserved exported protein of unknown function, at 1,420,521 to 1,421,411
_1245
Position (kb)
1419
1420
1421 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1418.744 kb on + strand, within PP_1241 at 1418.744 kb on + strand, within PP_1241 at 1418.745 kb on - strand, within PP_1241 at 1418.894 kb on + strand at 1418.894 kb on + strand at 1418.894 kb on + strand at 1418.955 kb on + strand at 1418.956 kb on - strand at 1418.977 kb on - strand at 1418.989 kb on + strand at 1418.989 kb on + strand at 1418.989 kb on + strand at 1418.989 kb on + strand at 1418.989 kb on + strand at 1418.990 kb on - strand at 1418.990 kb on - strand at 1418.990 kb on - strand at 1418.991 kb on + strand at 1418.991 kb on + strand at 1418.991 kb on + strand at 1418.991 kb on + strand at 1418.992 kb on - strand at 1419.118 kb on - strand at 1419.118 kb on - strand at 1419.127 kb on + strand at 1419.167 kb on + strand at 1419.211 kb on - strand at 1419.300 kb on + strand, within PP_1242 at 1419.301 kb on - strand, within PP_1242 at 1419.301 kb on - strand, within PP_1242 at 1419.366 kb on + strand, within PP_1242 at 1419.366 kb on + strand, within PP_1242 at 1419.366 kb on + strand, within PP_1242 at 1419.459 kb on + strand, within PP_1242 at 1419.459 kb on + strand, within PP_1242 at 1419.460 kb on - strand, within PP_1242 at 1419.460 kb on - strand, within PP_1242 at 1419.578 kb on + strand at 1419.579 kb on - strand at 1419.579 kb on - strand at 1419.649 kb on - strand at 1419.674 kb on + strand at 1419.674 kb on + strand at 1419.674 kb on + strand at 1419.675 kb on - strand at 1419.726 kb on - strand, within PP_1244 at 1419.824 kb on + strand, within PP_1244 at 1419.824 kb on + strand, within PP_1244 at 1419.824 kb on + strand, within PP_1244 at 1419.824 kb on + strand, within PP_1244 at 1419.825 kb on - strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244 at 1419.828 kb on + strand, within PP_1244 at 1419.904 kb on + strand, within PP_1244 at 1419.904 kb on + strand, within PP_1244 at 1419.905 kb on - strand, within PP_1244 at 1419.968 kb on - strand, within PP_1244 at 1419.968 kb on - strand, within PP_1244 at 1419.968 kb on - strand, within PP_1244 at 1419.968 kb on - strand, within PP_1244 at 1419.968 kb on - strand, within PP_1244 at 1419.968 kb on - strand, within PP_1244 at 1419.998 kb on + strand at 1420.027 kb on + strand at 1420.028 kb on - strand at 1420.157 kb on + strand at 1420.157 kb on + strand at 1420.158 kb on - strand at 1420.175 kb on + strand at 1420.190 kb on - strand at 1420.190 kb on - strand at 1420.190 kb on - strand at 1420.199 kb on + strand at 1420.208 kb on + strand at 1420.209 kb on - strand at 1420.209 kb on - strand at 1420.221 kb on + strand at 1420.225 kb on - strand at 1420.285 kb on - strand at 1420.285 kb on - strand at 1420.395 kb on + strand at 1420.395 kb on + strand at 1420.395 kb on + strand at 1420.557 kb on - strand at 1420.598 kb on + strand at 1420.599 kb on - strand at 1420.744 kb on - strand, within PP_1245 at 1420.912 kb on - strand, within PP_1245 at 1420.912 kb on - strand, within PP_1245 at 1420.992 kb on + strand, within PP_1245 at 1420.992 kb on + strand, within PP_1245 at 1420.993 kb on - strand, within PP_1245 at 1420.993 kb on - strand, within PP_1245 at 1420.993 kb on - strand, within PP_1245
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_4; with MOPS remove 1,418,744 + PP_1241 0.60 +1.7 1,418,744 + PP_1241 0.60 +0.1 1,418,745 - PP_1241 0.61 +0.7 1,418,894 + +0.5 1,418,894 + +0.1 1,418,894 + +0.2 1,418,955 + +0.3 1,418,956 - +0.8 1,418,977 - -0.1 1,418,989 + +0.8 1,418,989 + -0.2 1,418,989 + -0.2 1,418,989 + +0.9 1,418,989 + +1.4 1,418,990 - -0.0 1,418,990 - -0.2 1,418,990 - +0.6 1,418,991 + +0.7 1,418,991 + -0.2 1,418,991 + -1.5 1,418,991 + -0.4 1,418,992 - -0.0 1,419,118 - +0.3 1,419,118 - +0.4 1,419,127 + -0.5 1,419,167 + -1.4 1,419,211 - -0.3 1,419,300 + PP_1242 0.22 -0.0 1,419,301 - PP_1242 0.23 +2.6 1,419,301 - PP_1242 0.23 +0.5 1,419,366 + PP_1242 0.47 +0.5 1,419,366 + PP_1242 0.47 +0.4 1,419,366 + PP_1242 0.47 +1.4 1,419,459 + PP_1242 0.81 -0.9 1,419,459 + PP_1242 0.81 +0.3 1,419,460 - PP_1242 0.81 +0.7 1,419,460 - PP_1242 0.81 +0.4 1,419,578 + +0.2 1,419,579 - -2.4 1,419,579 - -0.1 1,419,649 - +0.3 1,419,674 + -0.7 1,419,674 + +0.7 1,419,674 + +0.6 1,419,675 - +0.4 1,419,726 - PP_1244 0.22 -0.3 1,419,824 + PP_1244 0.48 -0.1 1,419,824 + PP_1244 0.48 +1.9 1,419,824 + PP_1244 0.48 +0.0 1,419,824 + PP_1244 0.48 -0.4 1,419,825 - PP_1244 0.49 +0.2 1,419,828 + PP_1244 0.49 +0.3 1,419,828 + PP_1244 0.49 +1.0 1,419,828 + PP_1244 0.49 +0.9 1,419,828 + PP_1244 0.49 +0.0 1,419,828 + PP_1244 0.49 +0.3 1,419,904 + PP_1244 0.70 -0.1 1,419,904 + PP_1244 0.70 -0.5 1,419,905 - PP_1244 0.70 +1.0 1,419,968 - PP_1244 0.88 +1.8 1,419,968 - PP_1244 0.88 +1.2 1,419,968 - PP_1244 0.88 -0.6 1,419,968 - PP_1244 0.88 +1.6 1,419,968 - PP_1244 0.88 +1.2 1,419,968 - PP_1244 0.88 +1.0 1,419,998 + -0.9 1,420,027 + -0.1 1,420,028 - +0.7 1,420,157 + -0.4 1,420,157 + -0.1 1,420,158 - +0.3 1,420,175 + -0.9 1,420,190 - +1.1 1,420,190 - +0.5 1,420,190 - +0.6 1,420,199 + +0.0 1,420,208 + -0.5 1,420,209 - +1.2 1,420,209 - +0.7 1,420,221 + -1.2 1,420,225 - +0.1 1,420,285 - -0.9 1,420,285 - -0.4 1,420,395 + -0.8 1,420,395 + +0.4 1,420,395 + +0.8 1,420,557 - +0.8 1,420,598 + +0.2 1,420,599 - -1.1 1,420,744 - PP_1245 0.25 +0.4 1,420,912 - PP_1245 0.44 +0.3 1,420,912 - PP_1245 0.44 +0.2 1,420,992 + PP_1245 0.53 +0.6 1,420,992 + PP_1245 0.53 +0.5 1,420,993 - PP_1245 0.53 -0.3 1,420,993 - PP_1245 0.53 -1.0 1,420,993 - PP_1245 0.53 +0.3
Or see this region's nucleotide sequence