Experiment: Temperature shift 30_to_4; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3149 and PP_3150 overlap by 4 nucleotides PP_3150 and PP_3151 are separated by 37 nucleotides
PP_3149: PP_3149 - Transcriptional regulator, AraC family, at 3,566,398 to 3,567,447
_3149
PP_3150: PP_3150 - conserved protein of unknown function, at 3,567,444 to 3,569,441
_3150
PP_3151: PP_3151 - NAD+-dependent succinate semialdehyde dehydrogenase, at 3,569,479 to 3,570,864
_3151
Position (kb)
3567
3568
3569
3570 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3566.467 kb on - strand at 3566.467 kb on - strand at 3566.467 kb on - strand at 3566.496 kb on - strand at 3566.496 kb on - strand at 3566.496 kb on - strand at 3566.707 kb on + strand, within PP_3149 at 3566.708 kb on - strand, within PP_3149 at 3566.708 kb on - strand, within PP_3149 at 3566.800 kb on + strand, within PP_3149 at 3566.801 kb on - strand, within PP_3149 at 3566.801 kb on - strand, within PP_3149 at 3566.827 kb on - strand, within PP_3149 at 3566.849 kb on - strand, within PP_3149 at 3566.849 kb on - strand, within PP_3149 at 3566.849 kb on - strand, within PP_3149 at 3566.849 kb on - strand, within PP_3149 at 3566.849 kb on - strand, within PP_3149 at 3567.249 kb on + strand, within PP_3149 at 3567.250 kb on - strand, within PP_3149 at 3567.392 kb on - strand at 3567.498 kb on + strand at 3567.538 kb on - strand at 3567.763 kb on + strand, within PP_3150 at 3567.764 kb on - strand, within PP_3150 at 3567.852 kb on + strand, within PP_3150 at 3567.853 kb on - strand at 3567.945 kb on + strand, within PP_3150 at 3568.179 kb on + strand, within PP_3150 at 3568.180 kb on - strand, within PP_3150 at 3568.180 kb on - strand, within PP_3150 at 3568.180 kb on - strand, within PP_3150 at 3568.367 kb on + strand, within PP_3150 at 3568.549 kb on - strand, within PP_3150 at 3568.549 kb on - strand, within PP_3150 at 3568.549 kb on - strand, within PP_3150 at 3568.549 kb on - strand, within PP_3150 at 3568.683 kb on - strand, within PP_3150 at 3568.864 kb on - strand, within PP_3150 at 3569.052 kb on + strand, within PP_3150 at 3569.169 kb on + strand, within PP_3150 at 3569.343 kb on - strand at 3569.343 kb on - strand at 3569.359 kb on - strand at 3569.359 kb on - strand at 3569.359 kb on - strand at 3569.664 kb on - strand, within PP_3151 at 3569.675 kb on + strand, within PP_3151 at 3569.675 kb on + strand, within PP_3151 at 3569.676 kb on - strand, within PP_3151 at 3569.676 kb on - strand, within PP_3151 at 3569.676 kb on - strand, within PP_3151 at 3569.676 kb on - strand, within PP_3151 at 3569.676 kb on - strand, within PP_3151 at 3569.950 kb on - strand, within PP_3151 at 3569.951 kb on + strand, within PP_3151 at 3569.951 kb on + strand, within PP_3151 at 3569.951 kb on + strand, within PP_3151 at 3569.951 kb on + strand, within PP_3151 at 3569.951 kb on + strand, within PP_3151 at 3569.951 kb on + strand, within PP_3151 at 3569.951 kb on + strand, within PP_3151 at 3569.951 kb on + strand, within PP_3151 at 3569.951 kb on + strand, within PP_3151 at 3569.952 kb on - strand, within PP_3151 at 3569.952 kb on - strand, within PP_3151 at 3569.952 kb on - strand, within PP_3151 at 3569.952 kb on - strand, within PP_3151 at 3570.087 kb on - strand, within PP_3151 at 3570.359 kb on + strand, within PP_3151 at 3570.374 kb on - strand, within PP_3151 at 3570.398 kb on + strand, within PP_3151 at 3570.399 kb on - strand, within PP_3151 at 3570.399 kb on - strand, within PP_3151
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_4; with MOPS remove 3,566,467 - -0.0 3,566,467 - -0.7 3,566,467 - -0.2 3,566,496 - -0.4 3,566,496 - -0.3 3,566,496 - +0.4 3,566,707 + PP_3149 0.29 -0.3 3,566,708 - PP_3149 0.30 +0.1 3,566,708 - PP_3149 0.30 -0.6 3,566,800 + PP_3149 0.38 -0.2 3,566,801 - PP_3149 0.38 -0.2 3,566,801 - PP_3149 0.38 +2.0 3,566,827 - PP_3149 0.41 +0.3 3,566,849 - PP_3149 0.43 -0.4 3,566,849 - PP_3149 0.43 +0.8 3,566,849 - PP_3149 0.43 +0.0 3,566,849 - PP_3149 0.43 -0.0 3,566,849 - PP_3149 0.43 +0.0 3,567,249 + PP_3149 0.81 -0.2 3,567,250 - PP_3149 0.81 +0.0 3,567,392 - +1.0 3,567,498 + -1.5 3,567,538 - +1.9 3,567,763 + PP_3150 0.16 -0.7 3,567,764 - PP_3150 0.16 -3.1 3,567,852 + PP_3150 0.20 -0.8 3,567,853 - -1.3 3,567,945 + PP_3150 0.25 -0.7 3,568,179 + PP_3150 0.37 -0.9 3,568,180 - PP_3150 0.37 -1.0 3,568,180 - PP_3150 0.37 -0.9 3,568,180 - PP_3150 0.37 -0.6 3,568,367 + PP_3150 0.46 -3.2 3,568,549 - PP_3150 0.55 +0.0 3,568,549 - PP_3150 0.55 -0.5 3,568,549 - PP_3150 0.55 -1.6 3,568,549 - PP_3150 0.55 -0.7 3,568,683 - PP_3150 0.62 -0.5 3,568,864 - PP_3150 0.71 -2.0 3,569,052 + PP_3150 0.80 -1.2 3,569,169 + PP_3150 0.86 -0.6 3,569,343 - -1.8 3,569,343 - -0.2 3,569,359 - -1.3 3,569,359 - -1.2 3,569,359 - -3.1 3,569,664 - PP_3151 0.13 -0.6 3,569,675 + PP_3151 0.14 +0.1 3,569,675 + PP_3151 0.14 -0.0 3,569,676 - PP_3151 0.14 -1.6 3,569,676 - PP_3151 0.14 +0.8 3,569,676 - PP_3151 0.14 +0.6 3,569,676 - PP_3151 0.14 +1.5 3,569,676 - PP_3151 0.14 -0.4 3,569,950 - PP_3151 0.34 -0.1 3,569,951 + PP_3151 0.34 -0.0 3,569,951 + PP_3151 0.34 +0.0 3,569,951 + PP_3151 0.34 +0.4 3,569,951 + PP_3151 0.34 +0.1 3,569,951 + PP_3151 0.34 +0.4 3,569,951 + PP_3151 0.34 -0.1 3,569,951 + PP_3151 0.34 -0.5 3,569,951 + PP_3151 0.34 +0.1 3,569,951 + PP_3151 0.34 +0.7 3,569,952 - PP_3151 0.34 -0.8 3,569,952 - PP_3151 0.34 -0.6 3,569,952 - PP_3151 0.34 +0.5 3,569,952 - PP_3151 0.34 -0.1 3,570,087 - PP_3151 0.44 -0.2 3,570,359 + PP_3151 0.63 -2.0 3,570,374 - PP_3151 0.65 -1.0 3,570,398 + PP_3151 0.66 +0.1 3,570,399 - PP_3151 0.66 -1.1 3,570,399 - PP_3151 0.66 +0.6
Or see this region's nucleotide sequence