Strain Fitness in Pseudomonas putida KT2440 around PP_2024

Experiment: Temperature shift 30_to_4; with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_5496 and PP_2023 are separated by 132 nucleotidesPP_2023 and PP_2024 overlap by 10 nucleotidesPP_2024 and PP_2025 overlap by 4 nucleotides PP_5496: PP_5496 - conserved exported protein of unknown function, at 2,295,578 to 2,295,754 _5496 PP_2023: PP_2023 - Glutathione S-transferase family protein, at 2,295,887 to 2,296,486 _2023 PP_2024: PP_2024 - Exonuclease SbcC, at 2,296,477 to 2,300,121 _2024 PP_2025: PP_2025 - Exonuclease SbcD, at 2,300,118 to 2,301,356 _2025 Position (kb) 2296 2297 2298 2299 2300 2301Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2295.478 kb on + strandat 2295.570 kb on - strandat 2295.602 kb on - strand, within PP_5496at 2295.686 kb on + strand, within PP_5496at 2295.686 kb on + strand, within PP_5496at 2295.687 kb on - strand, within PP_5496at 2295.699 kb on - strand, within PP_5496at 2296.057 kb on - strand, within PP_2023at 2296.158 kb on + strandat 2296.158 kb on + strand, within PP_2023at 2296.158 kb on + strand, within PP_2023at 2296.158 kb on + strand, within PP_2023at 2296.242 kb on - strand, within PP_2023at 2296.562 kb on - strandat 2296.864 kb on + strand, within PP_2024at 2296.864 kb on + strand, within PP_2024at 2296.864 kb on + strand, within PP_2024at 2296.865 kb on - strand, within PP_2024at 2296.865 kb on - strand, within PP_2024at 2296.904 kb on + strand, within PP_2024at 2296.904 kb on + strand, within PP_2024at 2296.904 kb on + strand, within PP_2024at 2296.905 kb on - strand, within PP_2024at 2297.599 kb on + strand, within PP_2024at 2297.791 kb on + strand, within PP_2024at 2297.792 kb on - strand, within PP_2024at 2297.795 kb on - strand, within PP_2024at 2298.077 kb on - strand, within PP_2024at 2298.662 kb on + strand, within PP_2024at 2298.788 kb on + strand, within PP_2024at 2298.988 kb on - strand, within PP_2024at 2299.447 kb on + strand, within PP_2024at 2299.447 kb on + strand, within PP_2024at 2299.683 kb on - strand, within PP_2024at 2299.727 kb on + strand, within PP_2024at 2299.727 kb on + strand, within PP_2024at 2299.728 kb on - strand, within PP_2024at 2299.728 kb on - strand, within PP_2024at 2300.630 kb on - strand, within PP_2025at 2300.689 kb on + strand, within PP_2025at 2300.689 kb on + strand, within PP_2025at 2300.696 kb on + strand, within PP_2025at 2300.885 kb on - strand, within PP_2025

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Per-strain Table

Position Strand Gene LocusTag Fraction Temperature shift 30_to_4; with MOPS
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2,295,478 + -0.6
2,295,570 - -0.2
2,295,602 - PP_5496 0.14 -2.7
2,295,686 + PP_5496 0.61 +0.5
2,295,686 + PP_5496 0.61 -0.8
2,295,687 - PP_5496 0.62 +1.1
2,295,699 - PP_5496 0.68 -1.4
2,296,057 - PP_2023 0.28 +0.1
2,296,158 + +2.5
2,296,158 + PP_2023 0.45 -0.3
2,296,158 + PP_2023 0.45 -0.4
2,296,158 + PP_2023 0.45 +0.7
2,296,242 - PP_2023 0.59 -1.3
2,296,562 - -0.8
2,296,864 + PP_2024 0.11 -2.2
2,296,864 + PP_2024 0.11 -0.4
2,296,864 + PP_2024 0.11 -0.1
2,296,865 - PP_2024 0.11 -0.8
2,296,865 - PP_2024 0.11 -0.9
2,296,904 + PP_2024 0.12 -0.0
2,296,904 + PP_2024 0.12 -0.1
2,296,904 + PP_2024 0.12 -2.0
2,296,905 - PP_2024 0.12 -1.9
2,297,599 + PP_2024 0.31 -0.8
2,297,791 + PP_2024 0.36 +0.7
2,297,792 - PP_2024 0.36 -0.2
2,297,795 - PP_2024 0.36 -1.2
2,298,077 - PP_2024 0.44 -1.0
2,298,662 + PP_2024 0.60 -3.4
2,298,788 + PP_2024 0.63 -1.3
2,298,988 - PP_2024 0.69 -1.0
2,299,447 + PP_2024 0.81 -1.1
2,299,447 + PP_2024 0.81 -0.3
2,299,683 - PP_2024 0.88 -0.8
2,299,727 + PP_2024 0.89 -0.7
2,299,727 + PP_2024 0.89 -1.3
2,299,728 - PP_2024 0.89 -1.5
2,299,728 - PP_2024 0.89 -0.7
2,300,630 - PP_2025 0.41 -1.0
2,300,689 + PP_2025 0.46 -0.4
2,300,689 + PP_2025 0.46 -0.5
2,300,696 + PP_2025 0.47 +0.2
2,300,885 - PP_2025 0.62 +0.6

Or see this region's nucleotide sequence