Experiment: Temperature shift 30_to_4; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5496 and PP_2023 are separated by 132 nucleotides PP_2023 and PP_2024 overlap by 10 nucleotides PP_2024 and PP_2025 overlap by 4 nucleotides
PP_5496: PP_5496 - conserved exported protein of unknown function, at 2,295,578 to 2,295,754
_5496
PP_2023: PP_2023 - Glutathione S-transferase family protein, at 2,295,887 to 2,296,486
_2023
PP_2024: PP_2024 - Exonuclease SbcC, at 2,296,477 to 2,300,121
_2024
PP_2025: PP_2025 - Exonuclease SbcD, at 2,300,118 to 2,301,356
_2025
Position (kb)
2296
2297
2298
2299
2300
2301 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2295.478 kb on + strand at 2295.570 kb on - strand at 2295.602 kb on - strand, within PP_5496 at 2295.686 kb on + strand, within PP_5496 at 2295.686 kb on + strand, within PP_5496 at 2295.687 kb on - strand, within PP_5496 at 2295.699 kb on - strand, within PP_5496 at 2296.057 kb on - strand, within PP_2023 at 2296.158 kb on + strand at 2296.158 kb on + strand, within PP_2023 at 2296.158 kb on + strand, within PP_2023 at 2296.158 kb on + strand, within PP_2023 at 2296.242 kb on - strand, within PP_2023 at 2296.562 kb on - strand at 2296.864 kb on + strand, within PP_2024 at 2296.864 kb on + strand, within PP_2024 at 2296.864 kb on + strand, within PP_2024 at 2296.865 kb on - strand, within PP_2024 at 2296.865 kb on - strand, within PP_2024 at 2296.904 kb on + strand, within PP_2024 at 2296.904 kb on + strand, within PP_2024 at 2296.904 kb on + strand, within PP_2024 at 2296.905 kb on - strand, within PP_2024 at 2297.599 kb on + strand, within PP_2024 at 2297.791 kb on + strand, within PP_2024 at 2297.792 kb on - strand, within PP_2024 at 2297.795 kb on - strand, within PP_2024 at 2298.077 kb on - strand, within PP_2024 at 2298.662 kb on + strand, within PP_2024 at 2298.788 kb on + strand, within PP_2024 at 2298.988 kb on - strand, within PP_2024 at 2299.447 kb on + strand, within PP_2024 at 2299.447 kb on + strand, within PP_2024 at 2299.683 kb on - strand, within PP_2024 at 2299.727 kb on + strand, within PP_2024 at 2299.727 kb on + strand, within PP_2024 at 2299.728 kb on - strand, within PP_2024 at 2299.728 kb on - strand, within PP_2024 at 2300.630 kb on - strand, within PP_2025 at 2300.689 kb on + strand, within PP_2025 at 2300.689 kb on + strand, within PP_2025 at 2300.696 kb on + strand, within PP_2025 at 2300.885 kb on - strand, within PP_2025
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_4; with MOPS remove 2,295,478 + -0.6 2,295,570 - -0.2 2,295,602 - PP_5496 0.14 -2.7 2,295,686 + PP_5496 0.61 +0.5 2,295,686 + PP_5496 0.61 -0.8 2,295,687 - PP_5496 0.62 +1.1 2,295,699 - PP_5496 0.68 -1.4 2,296,057 - PP_2023 0.28 +0.1 2,296,158 + +2.5 2,296,158 + PP_2023 0.45 -0.3 2,296,158 + PP_2023 0.45 -0.4 2,296,158 + PP_2023 0.45 +0.7 2,296,242 - PP_2023 0.59 -1.3 2,296,562 - -0.8 2,296,864 + PP_2024 0.11 -2.2 2,296,864 + PP_2024 0.11 -0.4 2,296,864 + PP_2024 0.11 -0.1 2,296,865 - PP_2024 0.11 -0.8 2,296,865 - PP_2024 0.11 -0.9 2,296,904 + PP_2024 0.12 -0.0 2,296,904 + PP_2024 0.12 -0.1 2,296,904 + PP_2024 0.12 -2.0 2,296,905 - PP_2024 0.12 -1.9 2,297,599 + PP_2024 0.31 -0.8 2,297,791 + PP_2024 0.36 +0.7 2,297,792 - PP_2024 0.36 -0.2 2,297,795 - PP_2024 0.36 -1.2 2,298,077 - PP_2024 0.44 -1.0 2,298,662 + PP_2024 0.60 -3.4 2,298,788 + PP_2024 0.63 -1.3 2,298,988 - PP_2024 0.69 -1.0 2,299,447 + PP_2024 0.81 -1.1 2,299,447 + PP_2024 0.81 -0.3 2,299,683 - PP_2024 0.88 -0.8 2,299,727 + PP_2024 0.89 -0.7 2,299,727 + PP_2024 0.89 -1.3 2,299,728 - PP_2024 0.89 -1.5 2,299,728 - PP_2024 0.89 -0.7 2,300,630 - PP_2025 0.41 -1.0 2,300,689 + PP_2025 0.46 -0.4 2,300,689 + PP_2025 0.46 -0.5 2,300,696 + PP_2025 0.47 +0.2 2,300,885 - PP_2025 0.62 +0.6
Or see this region's nucleotide sequence