Experiment: Temperature shift 30_to_4; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0483 and PP_0484 are separated by 129 nucleotides PP_0484 and PP_0485 are separated by 9 nucleotides PP_0485 and PP_0486 are separated by 90 nucleotides
PP_0483: PP_0483 - excinuclease (uvrABC system protein A), at 566,661 to 569,495
_0483
PP_0484: PP_0484 - transporter of an unknown substrate, at 569,625 to 571,019
_0484
PP_0485: PP_0485 - Single-stranded DNA-binding protein, at 571,029 to 571,574
_0485
PP_0486: PP_0486 - Transcriptional regulator, GntR family, at 571,665 to 573,089
_0486
Position (kb) 569
570
571
572 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 569.518 kb on - strand at 569.529 kb on + strand at 569.530 kb on - strand at 569.530 kb on - strand at 569.567 kb on - strand at 569.705 kb on + strand at 569.706 kb on - strand at 569.734 kb on + strand at 569.745 kb on + strand at 569.802 kb on + strand, within PP_0484 at 569.802 kb on + strand, within PP_0484 at 569.803 kb on - strand, within PP_0484 at 569.803 kb on - strand, within PP_0484 at 569.878 kb on - strand, within PP_0484 at 569.911 kb on + strand, within PP_0484 at 569.911 kb on + strand, within PP_0484 at 569.912 kb on - strand, within PP_0484 at 569.912 kb on - strand, within PP_0484 at 569.933 kb on + strand, within PP_0484 at 570.393 kb on + strand, within PP_0484 at 570.394 kb on - strand at 570.394 kb on - strand, within PP_0484 at 570.444 kb on + strand, within PP_0484 at 570.444 kb on + strand, within PP_0484 at 570.444 kb on + strand, within PP_0484 at 570.445 kb on - strand, within PP_0484 at 570.445 kb on - strand, within PP_0484 at 570.445 kb on - strand, within PP_0484 at 570.514 kb on + strand, within PP_0484 at 570.515 kb on - strand, within PP_0484 at 570.516 kb on + strand, within PP_0484 at 570.516 kb on + strand, within PP_0484 at 570.516 kb on + strand, within PP_0484 at 570.663 kb on + strand, within PP_0484 at 570.773 kb on + strand, within PP_0484 at 570.813 kb on + strand, within PP_0484 at 570.813 kb on + strand, within PP_0484 at 570.813 kb on + strand, within PP_0484 at 570.936 kb on + strand at 570.936 kb on + strand at 570.938 kb on + strand at 571.669 kb on + strand at 571.890 kb on - strand, within PP_0486 at 571.890 kb on - strand, within PP_0486 at 571.890 kb on - strand, within PP_0486 at 571.921 kb on + strand, within PP_0486 at 571.922 kb on - strand, within PP_0486 at 571.979 kb on + strand, within PP_0486
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_4; with MOPS remove 569,518 - -1.4 569,529 + -1.2 569,530 - +0.1 569,530 - +0.2 569,567 - +0.7 569,705 + +0.1 569,706 - -3.0 569,734 + -1.1 569,745 + -1.4 569,802 + PP_0484 0.13 -2.1 569,802 + PP_0484 0.13 -1.5 569,803 - PP_0484 0.13 -0.7 569,803 - PP_0484 0.13 -2.0 569,878 - PP_0484 0.18 -0.4 569,911 + PP_0484 0.21 -1.9 569,911 + PP_0484 0.21 -1.2 569,912 - PP_0484 0.21 -1.5 569,912 - PP_0484 0.21 -0.9 569,933 + PP_0484 0.22 +1.4 570,393 + PP_0484 0.55 +0.4 570,394 - -0.2 570,394 - PP_0484 0.55 -1.9 570,444 + PP_0484 0.59 -2.7 570,444 + PP_0484 0.59 -2.1 570,444 + PP_0484 0.59 -1.8 570,445 - PP_0484 0.59 -2.1 570,445 - PP_0484 0.59 -0.6 570,445 - PP_0484 0.59 -2.1 570,514 + PP_0484 0.64 -0.7 570,515 - PP_0484 0.64 -0.1 570,516 + PP_0484 0.64 -0.9 570,516 + PP_0484 0.64 -1.8 570,516 + PP_0484 0.64 -0.1 570,663 + PP_0484 0.74 +0.8 570,773 + PP_0484 0.82 -1.1 570,813 + PP_0484 0.85 -1.2 570,813 + PP_0484 0.85 -0.2 570,813 + PP_0484 0.85 -1.3 570,936 + +0.1 570,936 + -2.5 570,938 + +1.0 571,669 + -1.1 571,890 - PP_0486 0.16 +1.6 571,890 - PP_0486 0.16 +0.1 571,890 - PP_0486 0.16 +0.5 571,921 + PP_0486 0.18 -1.7 571,922 - PP_0486 0.18 -1.4 571,979 + PP_0486 0.22 +1.1
Or see this region's nucleotide sequence