Experiment: R2A control with 0.2X Vogels
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04420 and PS417_04425 are separated by 3 nucleotides PS417_04425 and PS417_04430 are separated by 124 nucleotides
PS417_04420: PS417_04420 - histidinol dehydrogenase, at 982,018 to 983,340
_04420
PS417_04425: PS417_04425 - histidinol-phosphate aminotransferase, at 983,344 to 984,396
_04425
PS417_04430: PS417_04430 - uracil transporter, at 984,521 to 985,795
_04430
Position (kb) 983
984
985 Strain fitness (log2 ratio) -2
-1
0
1
2 at 982.360 kb on + strand, within PS417_04420 at 982.360 kb on + strand, within PS417_04420 at 982.423 kb on + strand, within PS417_04420 at 982.423 kb on + strand, within PS417_04420 at 982.628 kb on - strand, within PS417_04420 at 982.681 kb on + strand, within PS417_04420 at 982.681 kb on + strand, within PS417_04420 at 982.681 kb on + strand, within PS417_04420 at 982.682 kb on - strand, within PS417_04420 at 982.682 kb on - strand, within PS417_04420 at 982.893 kb on + strand, within PS417_04420 at 982.893 kb on + strand, within PS417_04420 at 982.893 kb on + strand, within PS417_04420 at 982.894 kb on - strand, within PS417_04420 at 982.894 kb on - strand at 982.894 kb on - strand, within PS417_04420 at 983.120 kb on - strand, within PS417_04420 at 983.188 kb on + strand, within PS417_04420 at 983.188 kb on + strand, within PS417_04420 at 983.189 kb on - strand, within PS417_04420 at 983.189 kb on - strand, within PS417_04420 at 983.189 kb on - strand, within PS417_04420 at 983.350 kb on - strand at 983.386 kb on + strand at 983.386 kb on + strand at 983.386 kb on + strand at 983.386 kb on + strand at 983.386 kb on + strand at 983.387 kb on - strand at 983.557 kb on + strand, within PS417_04425 at 983.558 kb on - strand, within PS417_04425 at 983.558 kb on - strand, within PS417_04425 at 983.558 kb on - strand, within PS417_04425 at 983.675 kb on - strand, within PS417_04425 at 983.823 kb on - strand, within PS417_04425 at 983.823 kb on - strand, within PS417_04425 at 983.894 kb on - strand, within PS417_04425 at 983.894 kb on - strand, within PS417_04425 at 983.894 kb on - strand, within PS417_04425 at 983.999 kb on + strand at 984.000 kb on - strand, within PS417_04425 at 984.124 kb on + strand, within PS417_04425 at 984.125 kb on - strand, within PS417_04425 at 984.163 kb on - strand, within PS417_04425 at 984.458 kb on + strand at 984.458 kb on + strand at 984.459 kb on - strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.469 kb on + strand at 984.470 kb on - strand at 984.519 kb on - strand at 984.523 kb on - strand at 984.523 kb on - strand at 984.842 kb on - strand, within PS417_04430 at 984.900 kb on + strand, within PS417_04430 at 984.900 kb on + strand, within PS417_04430 at 984.900 kb on + strand, within PS417_04430 at 984.939 kb on + strand, within PS417_04430 at 984.940 kb on - strand, within PS417_04430 at 984.940 kb on - strand at 985.210 kb on - strand, within PS417_04430 at 985.381 kb on + strand, within PS417_04430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control with 0.2X Vogels remove 982,360 + PS417_04420 0.26 +0.1 982,360 + PS417_04420 0.26 +0.1 982,423 + PS417_04420 0.31 +0.2 982,423 + PS417_04420 0.31 +0.4 982,628 - PS417_04420 0.46 -0.1 982,681 + PS417_04420 0.50 -0.0 982,681 + PS417_04420 0.50 -0.3 982,681 + PS417_04420 0.50 +0.1 982,682 - PS417_04420 0.50 +0.7 982,682 - PS417_04420 0.50 -1.2 982,893 + PS417_04420 0.66 -1.8 982,893 + PS417_04420 0.66 +0.1 982,893 + PS417_04420 0.66 -1.2 982,894 - PS417_04420 0.66 -0.2 982,894 - -0.4 982,894 - PS417_04420 0.66 -0.3 983,120 - PS417_04420 0.83 -1.0 983,188 + PS417_04420 0.88 +0.0 983,188 + PS417_04420 0.88 +0.4 983,189 - PS417_04420 0.89 +1.0 983,189 - PS417_04420 0.89 -0.1 983,189 - PS417_04420 0.89 +0.1 983,350 - +0.3 983,386 + -1.1 983,386 + -2.0 983,386 + +0.4 983,386 + -0.4 983,386 + +1.1 983,387 - -1.4 983,557 + PS417_04425 0.20 +0.7 983,558 - PS417_04425 0.20 -0.4 983,558 - PS417_04425 0.20 -0.5 983,558 - PS417_04425 0.20 +0.1 983,675 - PS417_04425 0.31 -0.1 983,823 - PS417_04425 0.45 +0.8 983,823 - PS417_04425 0.45 -0.3 983,894 - PS417_04425 0.52 -2.4 983,894 - PS417_04425 0.52 -0.1 983,894 - PS417_04425 0.52 +1.4 983,999 + +1.9 984,000 - PS417_04425 0.62 -1.0 984,124 + PS417_04425 0.74 +0.4 984,125 - PS417_04425 0.74 +0.4 984,163 - PS417_04425 0.78 -0.3 984,458 + +0.9 984,458 + -0.0 984,459 - -0.2 984,469 + +0.3 984,469 + +0.2 984,469 + -0.6 984,469 + -0.2 984,469 + -0.9 984,469 + +0.3 984,470 - +0.6 984,519 - -0.0 984,523 - -1.1 984,523 - -0.7 984,842 - PS417_04430 0.25 +1.4 984,900 + PS417_04430 0.30 -0.3 984,900 + PS417_04430 0.30 -0.1 984,900 + PS417_04430 0.30 +0.4 984,939 + PS417_04430 0.33 -0.2 984,940 - PS417_04430 0.33 -0.5 984,940 - +0.4 985,210 - PS417_04430 0.54 -0.6 985,381 + PS417_04430 0.67 -0.1
Or see this region's nucleotide sequence