Strain Fitness in Escherichia coli BW25113 around b4305

Experiment: T7_phage 0.00015625 MOI TC4

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	T7_phage 0.00015625 MOI TC4
4,527,642	+	sgcC	b4304	0.52	+2.1
4,527,648	-	sgcC	b4304	0.53	-0.4
4,527,702	+	sgcC	b4304	0.57	-2.6
4,527,702	+	sgcC	b4304	0.57	-2.3
4,527,752	-	sgcC	b4304	0.61	-1.0
4,527,850	-	sgcC	b4304	0.68	-0.5
4,527,875	+	sgcC	b4304	0.70	-0.3
4,527,875	+	sgcC	b4304	0.70	-1.2
4,527,940	+	sgcC	b4304	0.75	-0.7
4,528,011	-	sgcC	b4304	0.81	+0.3
4,528,090	-	sgcC	b4304	0.87	+2.1
4,528,092	+	sgcC	b4304	0.87	+0.0
4,528,092	+	sgcC	b4304	0.87	-0.2
4,528,120	+	sgcC	b4304	0.89	+0.2
4,528,125	+	sgcC	b4304	0.89	-2.2
4,528,152	+				+2.4
4,528,153	+				+1.8
4,528,153	+				+2.9
4,528,171	-				-0.3
4,528,185	-				-0.5
4,528,185	-				-0.3
4,528,187	-				+0.1
4,528,194	+				-1.6
4,528,202	-				-1.0
4,528,218	+				+0.2
4,528,221	-				-0.7
4,528,251	+				-0.6
4,528,264	+				+2.3
4,528,264	+				+1.5
4,528,292	-				-2.2
4,528,307	+	sgcB	b4565	0.10	+2.4
4,528,307	+	sgcB	b4565	0.10	+1.2
4,528,328	+	sgcB	b4565	0.18	+3.0
4,528,328	+	sgcB	b4565	0.18	+2.1
4,528,329	-	sgcB	b4565	0.18	-2.4
4,528,330	+	sgcB	b4565	0.19	-0.5
4,528,330	+	sgcB	b4565	0.19	-0.5
4,528,330	+	sgcB	b4565	0.19	-0.5
4,528,358	-	sgcB	b4565	0.29	+0.1
4,528,393	-	sgcB	b4565	0.41	-2.8
4,528,393	-	sgcB	b4565	0.41	+0.5
4,528,411	-	sgcB	b4565	0.48	-1.2
4,528,411	-	sgcB	b4565	0.48	-0.5
4,528,412	-	sgcB	b4565	0.48	-0.7
4,528,412	-	sgcB	b4565	0.48	+0.5
4,528,424	+	sgcB	b4565	0.52	+0.4
4,528,451	-	sgcB	b4565	0.62	-0.1
4,528,573	+				+0.5
4,528,575	+				-1.0
4,528,575	+				-1.2
4,528,578	-				-2.6
4,528,578	-				+0.8
4,528,594	+				-1.8
4,528,698	-	sgcX	b4305	0.13	+1.2
4,528,700	+	sgcX	b4305	0.13	-0.2
4,528,707	+	sgcX	b4305	0.14	+0.1
4,528,707	+	sgcX	b4305	0.14	-0.8
4,528,848	+	sgcX	b4305	0.26	+2.2
4,528,848	+	sgcX	b4305	0.26	+1.0
4,528,953	+	sgcX	b4305	0.36	-3.3
4,528,960	-	sgcX	b4305	0.36	+0.9
4,528,966	-	sgcX	b4305	0.37	+2.2
4,529,011	-	sgcX	b4305	0.41	-0.7
4,529,039	+	sgcX	b4305	0.43	+0.1
4,529,062	+	sgcX	b4305	0.45	-0.7
4,529,080	+	sgcX	b4305	0.47	+1.0
4,529,080	+	sgcX	b4305	0.47	+2.8
4,529,080	-	sgcX	b4305	0.47	+0.9
4,529,080	-	sgcX	b4305	0.47	+1.1
4,529,088	-	sgcX	b4305	0.48	+0.4
4,529,098	-	sgcX	b4305	0.49	+2.6
4,529,121	-	sgcX	b4305	0.51	-1.3
4,529,123	+	sgcX	b4305	0.51	-0.2
4,529,161	-	sgcX	b4305	0.54	-3.3
4,529,183	+	sgcX	b4305	0.56	+2.0
4,529,189	-	sgcX	b4305	0.57	-1.0
4,529,189	-	sgcX	b4305	0.57	+1.8
4,529,189	-	sgcX	b4305	0.57	-0.6
4,529,208	+	sgcX	b4305	0.58	+2.9
4,529,208	+	sgcX	b4305	0.58	+0.2
4,529,209	+	sgcX	b4305	0.58	-2.6
4,529,221	+	sgcX	b4305	0.60	+0.8
4,529,229	-	sgcX	b4305	0.60	+0.2
4,529,310	+	sgcX	b4305	0.67	+2.5
4,529,326	+	sgcX	b4305	0.69	+1.7
4,529,326	+	sgcX	b4305	0.69	-0.5
4,529,336	+	sgcX	b4305	0.70	+2.4
4,529,336	+	sgcX	b4305	0.70	+2.9
4,529,336	+	sgcX	b4305	0.70	+0.3
4,529,344	-	sgcX	b4305	0.70	-1.5
4,529,345	-	sgcX	b4305	0.71	-0.1
4,529,360	+	sgcX	b4305	0.72	-0.1
4,529,403	-	sgcX	b4305	0.76	-2.4
4,529,517	-	sgcX	b4305	0.86	+2.7
4,529,517	-	sgcX	b4305	0.86	+2.1
4,529,688	-				-2.5
4,529,688	-				-1.7
4,529,771	+				+0.0
4,529,806	+				-0.2
4,529,806	+				+0.1
4,529,838	+				-1.4
4,529,851	+				+1.0
4,529,851	-				+0.2
4,529,851	-				+0.1
4,529,895	+				-1.4
4,529,903	+				+0.7
4,529,903	-				+2.0
4,529,948	+				-1.4
4,529,963	-				-1.9
4,529,963	-				-0.4
4,530,060	-				+1.7
4,530,063	+				+0.0
4,530,071	-				+1.7
4,530,173	+	yjhY	b4656	0.38	-0.2
4,530,226	+	yjhY	b4656	0.59	-0.4
4,530,249	-	yjhY	b4656	0.67	-0.9
4,530,249	-	yjhY	b4656	0.67	+1.0
4,530,271	+	yjhY	b4656	0.76	-0.8
4,530,273	+	yjhY	b4656	0.77	+0.6
4,530,306	+	yjhY	b4656	0.89	-0.3
4,530,306	+	yjhY	b4656	0.89	-1.0
4,530,331	+				-0.3
4,530,341	+				-1.2
4,530,342	+				+1.4
4,530,482	-				+2.5
4,530,482	-				+1.5
4,530,553	-	yjhP	b4306	0.12	-0.7
4,530,617	+	yjhP	b4306	0.21	-0.2
4,530,644	-	yjhP	b4306	0.25	+0.9