Experiment: T7_phage 0.00015625 MOI TC4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjdA and yjcZ overlap by 4 nucleotides yjcZ and proP are separated by 263 nucleotides
b4109: yjdA - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 4,325,158 to 4,327,386
yjdA
b4110: yjcZ - orf, hypothetical protein (VIMSS), at 4,327,383 to 4,328,261
yjcZ
b4111: proP - proline/glycine betaine transporter (NCBI), at 4,328,525 to 4,330,027
proP
Position (kb)
4327
4328
4329 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4326.398 kb on - strand, within yjdA at 4326.446 kb on - strand, within yjdA at 4326.612 kb on - strand, within yjdA at 4326.683 kb on + strand, within yjdA at 4326.734 kb on - strand, within yjdA at 4326.734 kb on - strand, within yjdA at 4326.766 kb on - strand, within yjdA at 4326.904 kb on + strand, within yjdA at 4326.912 kb on + strand, within yjdA at 4326.963 kb on + strand, within yjdA at 4327.073 kb on + strand, within yjdA at 4327.078 kb on + strand, within yjdA at 4327.078 kb on + strand, within yjdA at 4327.238 kb on + strand at 4327.238 kb on + strand at 4327.238 kb on + strand at 4327.238 kb on + strand at 4327.246 kb on - strand at 4327.246 kb on - strand at 4327.277 kb on - strand at 4327.406 kb on + strand at 4327.406 kb on + strand at 4327.406 kb on + strand at 4327.458 kb on - strand at 4327.468 kb on - strand at 4327.483 kb on - strand, within yjcZ at 4327.488 kb on + strand, within yjcZ at 4327.522 kb on + strand, within yjcZ at 4327.522 kb on + strand, within yjcZ at 4327.634 kb on - strand, within yjcZ at 4327.663 kb on + strand, within yjcZ at 4327.716 kb on - strand, within yjcZ at 4327.739 kb on + strand, within yjcZ at 4327.793 kb on + strand, within yjcZ at 4327.794 kb on + strand, within yjcZ at 4327.823 kb on - strand, within yjcZ at 4327.853 kb on + strand, within yjcZ at 4327.902 kb on + strand, within yjcZ at 4327.902 kb on + strand, within yjcZ at 4327.928 kb on + strand, within yjcZ at 4328.009 kb on + strand, within yjcZ at 4328.039 kb on + strand, within yjcZ at 4328.039 kb on + strand, within yjcZ at 4328.039 kb on + strand, within yjcZ at 4328.183 kb on + strand at 4328.200 kb on + strand at 4328.208 kb on - strand at 4328.208 kb on - strand at 4328.267 kb on + strand at 4328.267 kb on + strand at 4328.555 kb on + strand at 4328.555 kb on + strand at 4328.615 kb on + strand at 4328.729 kb on - strand, within proP at 4328.729 kb on - strand, within proP at 4328.729 kb on - strand, within proP at 4328.834 kb on + strand, within proP at 4328.834 kb on + strand, within proP at 4328.834 kb on - strand, within proP at 4328.840 kb on + strand, within proP at 4328.849 kb on + strand, within proP at 4328.854 kb on + strand, within proP at 4328.865 kb on + strand, within proP at 4328.865 kb on + strand, within proP at 4328.865 kb on + strand, within proP at 4328.865 kb on + strand, within proP at 4328.873 kb on - strand, within proP at 4328.873 kb on - strand, within proP at 4328.888 kb on + strand, within proP at 4328.888 kb on + strand, within proP at 4328.917 kb on + strand, within proP at 4328.954 kb on + strand, within proP at 4328.966 kb on - strand, within proP at 4328.986 kb on + strand, within proP at 4329.118 kb on + strand, within proP at 4329.144 kb on - strand, within proP at 4329.185 kb on + strand, within proP at 4329.237 kb on + strand, within proP at 4329.245 kb on + strand, within proP
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4 remove 4,326,398 - yjdA b4109 0.56 -1.3 4,326,446 - yjdA b4109 0.58 +2.2 4,326,612 - yjdA b4109 0.65 -0.3 4,326,683 + yjdA b4109 0.68 -1.3 4,326,734 - yjdA b4109 0.71 -0.8 4,326,734 - yjdA b4109 0.71 +0.0 4,326,766 - yjdA b4109 0.72 -1.1 4,326,904 + yjdA b4109 0.78 -1.3 4,326,912 + yjdA b4109 0.79 +0.1 4,326,963 + yjdA b4109 0.81 +2.0 4,327,073 + yjdA b4109 0.86 +2.2 4,327,078 + yjdA b4109 0.86 -1.2 4,327,078 + yjdA b4109 0.86 -0.9 4,327,238 + -1.7 4,327,238 + -0.6 4,327,238 + +1.2 4,327,238 + +0.3 4,327,246 - -1.5 4,327,246 - +0.2 4,327,277 - -0.2 4,327,406 + +0.4 4,327,406 + -0.7 4,327,406 + +0.2 4,327,458 - -0.6 4,327,468 - +1.2 4,327,483 - yjcZ b4110 0.11 -0.5 4,327,488 + yjcZ b4110 0.12 -0.8 4,327,522 + yjcZ b4110 0.16 -3.5 4,327,522 + yjcZ b4110 0.16 -2.6 4,327,634 - yjcZ b4110 0.29 +1.3 4,327,663 + yjcZ b4110 0.32 +0.4 4,327,716 - yjcZ b4110 0.38 +0.4 4,327,739 + yjcZ b4110 0.41 -2.8 4,327,793 + yjcZ b4110 0.47 -0.6 4,327,794 + yjcZ b4110 0.47 -1.0 4,327,823 - yjcZ b4110 0.50 -0.5 4,327,853 + yjcZ b4110 0.53 -1.7 4,327,902 + yjcZ b4110 0.59 -0.6 4,327,902 + yjcZ b4110 0.59 -1.0 4,327,928 + yjcZ b4110 0.62 -0.5 4,328,009 + yjcZ b4110 0.71 -0.9 4,328,039 + yjcZ b4110 0.75 +0.3 4,328,039 + yjcZ b4110 0.75 -2.7 4,328,039 + yjcZ b4110 0.75 -0.3 4,328,183 + -0.6 4,328,200 + -0.9 4,328,208 - -0.1 4,328,208 - +2.0 4,328,267 + -1.6 4,328,267 + -1.9 4,328,555 + -0.5 4,328,555 + +0.8 4,328,615 + -2.5 4,328,729 - proP b4111 0.14 -0.7 4,328,729 - proP b4111 0.14 +1.0 4,328,729 - proP b4111 0.14 +1.8 4,328,834 + proP b4111 0.21 -1.1 4,328,834 + proP b4111 0.21 +0.1 4,328,834 - proP b4111 0.21 +2.2 4,328,840 + proP b4111 0.21 -1.2 4,328,849 + proP b4111 0.22 -0.9 4,328,854 + proP b4111 0.22 +1.1 4,328,865 + proP b4111 0.23 -0.1 4,328,865 + proP b4111 0.23 +0.0 4,328,865 + proP b4111 0.23 -0.2 4,328,865 + proP b4111 0.23 -0.8 4,328,873 - proP b4111 0.23 -2.8 4,328,873 - proP b4111 0.23 -2.7 4,328,888 + proP b4111 0.24 +1.7 4,328,888 + proP b4111 0.24 +1.6 4,328,917 + proP b4111 0.26 -1.5 4,328,954 + proP b4111 0.29 -0.4 4,328,966 - proP b4111 0.29 -1.7 4,328,986 + proP b4111 0.31 -0.2 4,329,118 + proP b4111 0.39 +0.1 4,329,144 - proP b4111 0.41 -0.5 4,329,185 + proP b4111 0.44 +0.0 4,329,237 + proP b4111 0.47 -0.5 4,329,245 + proP b4111 0.48 +1.5
Or see this region's nucleotide sequence