Strain Fitness in Escherichia coli BW25113 around b4075

Experiment: T7_phage 0.00015625 MOI TC4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnrfE and nrfF overlap by 8 nucleotidesnrfF and nrfG overlap by 4 nucleotidesnrfG and gltP are separated by 341 nucleotides b4074: nrfE - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE (NCBI), at 4,289,535 to 4,291,193 nrfE b4075: nrfF - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF (NCBI), at 4,291,186 to 4,291,569 nrfF b4076: nrfG - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI), at 4,291,566 to 4,292,162 nrfG b4077: gltP - glutamate/aspartate:proton symporter (NCBI), at 4,292,504 to 4,293,817 gltP Position (kb) 4291 4292Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4290.255 kb on + strand, within nrfEat 4290.255 kb on + strand, within nrfEat 4290.265 kb on - strand, within nrfEat 4290.415 kb on - strand, within nrfEat 4290.415 kb on - strand, within nrfEat 4290.441 kb on - strand, within nrfEat 4290.443 kb on + strand, within nrfEat 4290.443 kb on + strand, within nrfEat 4290.465 kb on + strand, within nrfEat 4290.465 kb on - strand, within nrfEat 4290.465 kb on - strand, within nrfEat 4290.566 kb on + strand, within nrfEat 4290.572 kb on - strand, within nrfEat 4290.619 kb on + strand, within nrfEat 4290.619 kb on + strand, within nrfEat 4290.726 kb on + strand, within nrfEat 4290.797 kb on - strand, within nrfEat 4290.832 kb on + strand, within nrfEat 4290.839 kb on + strand, within nrfEat 4290.839 kb on + strand, within nrfEat 4290.847 kb on - strand, within nrfEat 4290.930 kb on + strand, within nrfEat 4290.952 kb on + strand, within nrfEat 4291.075 kb on + strandat 4291.075 kb on + strandat 4291.155 kb on + strandat 4291.208 kb on - strandat 4291.236 kb on + strand, within nrfFat 4291.270 kb on + strand, within nrfFat 4291.423 kb on - strand, within nrfFat 4291.464 kb on + strand, within nrfFat 4291.472 kb on - strand, within nrfFat 4291.483 kb on + strand, within nrfFat 4291.497 kb on + strand, within nrfFat 4291.503 kb on + strand, within nrfFat 4291.503 kb on + strand, within nrfFat 4291.711 kb on - strand, within nrfGat 4291.800 kb on - strand, within nrfGat 4291.854 kb on + strand, within nrfGat 4291.859 kb on + strand, within nrfGat 4291.911 kb on + strand, within nrfGat 4291.911 kb on + strand, within nrfGat 4291.934 kb on + strand, within nrfGat 4291.982 kb on + strand, within nrfGat 4291.982 kb on + strand, within nrfGat 4292.106 kb on + strandat 4292.109 kb on + strandat 4292.112 kb on - strandat 4292.112 kb on - strandat 4292.114 kb on + strandat 4292.114 kb on + strandat 4292.131 kb on - strandat 4292.368 kb on + strandat 4292.368 kb on + strandat 4292.388 kb on + strandat 4292.479 kb on + strandat 4292.479 kb on + strandat 4292.479 kb on + strandat 4292.479 kb on + strandat 4292.479 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4
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4,290,255 + nrfE b4074 0.43 -0.0
4,290,255 + nrfE b4074 0.43 +0.1
4,290,265 - nrfE b4074 0.44 -0.4
4,290,415 - nrfE b4074 0.53 -0.5
4,290,415 - nrfE b4074 0.53 -0.3
4,290,441 - nrfE b4074 0.55 -0.6
4,290,443 + nrfE b4074 0.55 -0.2
4,290,443 + nrfE b4074 0.55 -1.4
4,290,465 + nrfE b4074 0.56 -1.1
4,290,465 - nrfE b4074 0.56 -0.8
4,290,465 - nrfE b4074 0.56 -0.4
4,290,566 + nrfE b4074 0.62 +1.2
4,290,572 - nrfE b4074 0.63 +1.6
4,290,619 + nrfE b4074 0.65 +0.3
4,290,619 + nrfE b4074 0.65 +2.6
4,290,726 + nrfE b4074 0.72 -1.1
4,290,797 - nrfE b4074 0.76 +2.4
4,290,832 + nrfE b4074 0.78 -2.7
4,290,839 + nrfE b4074 0.79 +0.5
4,290,839 + nrfE b4074 0.79 -1.7
4,290,847 - nrfE b4074 0.79 +2.8
4,290,930 + nrfE b4074 0.84 -1.7
4,290,952 + nrfE b4074 0.85 +1.2
4,291,075 + -0.5
4,291,075 + +1.0
4,291,155 + -0.6
4,291,208 - -2.7
4,291,236 + nrfF b4075 0.13 +2.0
4,291,270 + nrfF b4075 0.22 +0.0
4,291,423 - nrfF b4075 0.62 +2.2
4,291,464 + nrfF b4075 0.72 -0.0
4,291,472 - nrfF b4075 0.74 +0.6
4,291,483 + nrfF b4075 0.77 +2.3
4,291,497 + nrfF b4075 0.81 +1.9
4,291,503 + nrfF b4075 0.83 +0.6
4,291,503 + nrfF b4075 0.83 +0.1
4,291,711 - nrfG b4076 0.24 -1.6
4,291,800 - nrfG b4076 0.39 +2.0
4,291,854 + nrfG b4076 0.48 -0.8
4,291,859 + nrfG b4076 0.49 -0.5
4,291,911 + nrfG b4076 0.58 +2.0
4,291,911 + nrfG b4076 0.58 +2.1
4,291,934 + nrfG b4076 0.62 -0.1
4,291,982 + nrfG b4076 0.70 -0.2
4,291,982 + nrfG b4076 0.70 -1.6
4,292,106 + -1.1
4,292,109 + -1.8
4,292,112 - -1.1
4,292,112 - -0.5
4,292,114 + -1.7
4,292,114 + +0.7
4,292,131 - -2.1
4,292,368 + -1.6
4,292,368 + -1.9
4,292,388 + +0.2
4,292,479 + -2.5
4,292,479 + -0.2
4,292,479 + +0.2
4,292,479 + -1.0
4,292,479 + -1.2

Or see this region's nucleotide sequence