Strain Fitness in Escherichia coli BW25113 around b3513

Experiment: T7_phage 0.00015625 MOI TC4

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	T7_phage 0.00015625 MOI TC4
3,656,710	+	GadE	b3512	0.61	+3.3
3,656,710	+	GadE	b3512	0.61	+3.3
3,656,710	+	GadE	b3512	0.61	+3.3
3,656,710	+	GadE	b3512	0.61	+3.3
3,656,792	+	GadE	b3512	0.76	+3.3
3,656,792	+	GadE	b3512	0.76	+3.3
3,656,832	-	GadE	b3512	0.84	+1.3
3,656,848	+	GadE	b3512	0.87	+3.3
3,656,858	-	GadE	b3512	0.89	-4.7
3,656,871	+				+1.0
3,656,872	+				+1.3
3,656,872	+				+0.0
3,656,880	-				+0.2
3,656,880	-				-0.6
3,656,880	-				-1.3
3,656,880	-				+2.2
3,656,885	+				+1.3
3,656,885	+				+1.0
3,656,885	+				+2.3
3,656,886	-				+0.2
3,656,895	+				-0.7
3,656,895	+				-2.5
3,656,903	-				-1.4
3,656,904	-				+0.5
3,656,920	+				+3.3
3,656,923	+				+3.3
3,656,923	+				+0.5
3,656,928	-				-0.1
3,656,939	-				-2.0
3,656,939	-				+1.1
3,656,948	+				+2.9
3,656,949	+				+1.2
3,656,952	+				+1.2
3,656,953	+				-2.0
3,656,957	+				-0.9
3,656,958	+				+2.7
3,656,960	+				+1.5
3,656,961	+				+1.9
3,656,966	-				+0.8
3,656,966	-				-0.8
3,656,992	+				+0.1
3,656,992	+				-1.1
3,656,992	+				-0.4
3,656,997	+				+1.2
3,656,997	+				-1.5
3,657,000	-				+0.5
3,657,000	-				+2.9
3,657,003	-				-2.0
3,657,012	-				-1.8
3,657,022	-				+3.3
3,657,027	+				-0.2
3,657,032	+				+0.4
3,657,039	+				-1.0
3,657,039	+				-1.0
3,657,039	+				+0.2
3,657,053	+				-1.1
3,657,072	+				+0.5
3,657,122	-				+1.6
3,657,125	-				+1.8
3,657,131	+				+2.1
3,657,131	-				-1.8
3,657,147	+				-1.5
3,657,149	-				+2.6
3,657,168	-				-2.9
3,657,199	-				+0.2
3,657,285	+				-2.8
3,657,295	-				+0.5
3,657,387	-	mdtE	b3513	0.11	+1.1
3,657,448	+	mdtE	b3513	0.17	-0.1
3,657,453	+	mdtE	b3513	0.17	-2.6
3,657,456	-	mdtE	b3513	0.17	-1.0
3,657,456	-	mdtE	b3513	0.17	-0.5
3,657,463	+	mdtE	b3513	0.18	+1.0
3,657,552	+	mdtE	b3513	0.26	-3.1
3,657,552	+	mdtE	b3513	0.26	-0.7
3,657,560	-	mdtE	b3513	0.26	-0.5
3,657,560	-	mdtE	b3513	0.26	-1.1
3,657,560	-	mdtE	b3513	0.26	+0.4
3,657,560	-	mdtE	b3513	0.26	-1.3
3,657,604	-	mdtE	b3513	0.30	-0.1
3,657,604	-	mdtE	b3513	0.30	-0.8
3,657,646	-	mdtE	b3513	0.34	-0.5
3,657,674	-	mdtE	b3513	0.36	-2.1
3,657,707	+	mdtE	b3513	0.39	-0.6
3,657,853	+	mdtE	b3513	0.52	-2.6
3,657,853	+	mdtE	b3513	0.52	-1.0
3,657,853	+	mdtE	b3513	0.52	-0.3
3,657,908	-	mdtE	b3513	0.56	-0.4
3,657,908	-	mdtE	b3513	0.56	-0.3
3,657,919	+	mdtE	b3513	0.57	+0.5
3,657,967	+	mdtE	b3513	0.61	-0.8
3,657,967	+	mdtE	b3513	0.61	-2.8
3,657,967	+	mdtE	b3513	0.61	-0.9
3,657,967	+	mdtE	b3513	0.61	-3.2
3,657,967	+	mdtE	b3513	0.61	+1.1
3,657,975	-	mdtE	b3513	0.62	-2.6
3,657,975	-	mdtE	b3513	0.62	+0.8
3,657,975	-	mdtE	b3513	0.62	-1.7
3,658,002	-	mdtE	b3513	0.65	-2.6
3,658,005	+	mdtE	b3513	0.65	-0.1
3,658,049	-	mdtE	b3513	0.69	-0.2
3,658,049	-	mdtE	b3513	0.69	-1.2
3,658,110	+	mdtE	b3513	0.74	-0.6
3,658,129	-	mdtE	b3513	0.75	+0.1
3,658,129	-	mdtE	b3513	0.75	-0.7
3,658,131	-	mdtE	b3513	0.76	-0.2
3,658,133	-	mdtE	b3513	0.76	+0.2
3,658,133	-	mdtE	b3513	0.76	-0.5
3,658,191	+	mdtE	b3513	0.81	+0.3
3,658,191	+	mdtE	b3513	0.81	+0.7
3,658,209	-	mdtE	b3513	0.82	+2.3
3,658,259	+	mdtE	b3513	0.87	-0.2
3,658,284	+	mdtE	b3513	0.89	+0.5
3,658,417	+				+1.0
3,658,420	+				-2.7
3,658,423	-				-1.8
3,658,428	-				-1.2
3,658,428	-				+0.3
3,658,515	+				+1.6
3,658,578	+				+3.2
3,658,578	+				-0.0
3,658,609	-				+0.0
3,658,623	-				-3.1
3,658,623	-				-0.7
3,658,639	+				+2.5
3,658,639	+				-1.7
3,658,645	-				-0.5
3,658,656	+				+4.2
3,658,656	+				+1.0
3,658,670	-				+0.3
3,658,692	+				-0.5
3,658,692	+				-2.0
3,658,791	-	mdtF	b3514	0.11	-1.5
3,658,800	+	mdtF	b3514	0.12	-1.7
3,658,829	+	mdtF	b3514	0.13	-1.9
3,658,837	+	mdtF	b3514	0.13	-1.1
3,658,887	-	mdtF	b3514	0.14	+2.2
3,658,889	+	mdtF	b3514	0.15	-2.4
3,658,904	+	mdtF	b3514	0.15	-0.5
3,658,904	+	mdtF	b3514	0.15	-0.6
3,658,904	-	mdtF	b3514	0.15	-1.4
3,658,904	-	mdtF	b3514	0.15	-1.7
3,658,906	+	mdtF	b3514	0.15	-1.2
3,658,929	+	mdtF	b3514	0.16	-0.4
3,658,929	+	mdtF	b3514	0.16	-1.1
3,658,934	+	mdtF	b3514	0.16	-1.7
3,658,991	+	mdtF	b3514	0.18	+2.0
3,659,034	-	mdtF	b3514	0.19	-1.2
3,659,096	-	mdtF	b3514	0.21	-0.3
3,659,105	+	mdtF	b3514	0.21	+1.8
3,659,105	+	mdtF	b3514	0.21	+1.2
3,659,148	-	mdtF	b3514	0.23	+1.5
3,659,148	-	mdtF	b3514	0.23	+2.0
3,659,237	+	mdtF	b3514	0.26	-3.3
3,659,237	+	mdtF	b3514	0.26	-1.2
3,659,237	+	mdtF	b3514	0.26	+0.2
3,659,274	+	mdtF	b3514	0.27	-0.0
3,659,274	+	mdtF	b3514	0.27	-1.1
3,659,274	+	mdtF	b3514	0.27	-0.6
3,659,313	-	mdtF	b3514	0.28	-1.1
3,659,313	-	mdtF	b3514	0.28	+2.2
3,659,350	-	mdtF	b3514	0.29	+2.5
3,659,350	-	mdtF	b3514	0.29	+2.0
3,659,360	-	mdtF	b3514	0.30	-2.6
3,659,364	-	mdtF	b3514	0.30	-0.6