Strain Fitness in Escherichia coli BW25113 around b3061

Experiment: T7_phage 0.00015625 MOI TC4

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	T7_phage 0.00015625 MOI TC4
3,203,507	+	ygiP	b3060	0.17	+2.3
3,203,525	-	ygiP	b3060	0.19	-2.6
3,203,531	+	ygiP	b3060	0.20	+1.4
3,203,560	-	ygiP	b3060	0.23	-2.6
3,203,560	-	ygiP	b3060	0.23	-0.9
3,203,580	+	ygiP	b3060	0.25	+0.3
3,203,609	+	ygiP	b3060	0.28	-1.4
3,203,609	-	ygiP	b3060	0.28	+0.2
3,203,609	-	ygiP	b3060	0.28	-0.3
3,203,617	+	ygiP	b3060	0.29	-1.9
3,203,617	-	ygiP	b3060	0.29	+0.0
3,203,617	-	ygiP	b3060	0.29	+0.4
3,203,618	+	ygiP	b3060	0.29	+1.2
3,203,618	+	ygiP	b3060	0.29	-0.8
3,203,626	-	ygiP	b3060	0.30	+2.3
3,203,642	+	ygiP	b3060	0.32	+0.3
3,203,642	+	ygiP	b3060	0.32	-1.1
3,203,650	+	ygiP	b3060	0.33	+2.7
3,203,650	+	ygiP	b3060	0.33	-0.7
3,203,666	+	ygiP	b3060	0.34	+1.4
3,203,666	+	ygiP	b3060	0.34	-1.7
3,203,673	+	ygiP	b3060	0.35	+2.2
3,203,674	+	ygiP	b3060	0.35	+0.2
3,203,674	+	ygiP	b3060	0.35	-2.3
3,203,674	-	ygiP	b3060	0.35	+1.4
3,203,674	-	ygiP	b3060	0.35	+0.2
3,203,674	-	ygiP	b3060	0.35	+1.6
3,203,677	+	ygiP	b3060	0.35	+2.5
3,203,682	-	ygiP	b3060	0.36	-0.9
3,203,685	+	ygiP	b3060	0.36	+1.9
3,203,685	+	ygiP	b3060	0.36	-0.4
3,203,685	+	ygiP	b3060	0.36	+2.0
3,203,685	+	ygiP	b3060	0.36	-0.7
3,203,689	+	ygiP	b3060	0.37	+0.4
3,203,689	+	ygiP	b3060	0.37	-1.9
3,203,695	+	ygiP	b3060	0.37	+0.0
3,203,695	-	ygiP	b3060	0.37	+1.2
3,203,696	+	ygiP	b3060	0.38	+0.8
3,203,698	+	ygiP	b3060	0.38	-1.4
3,203,698	+	ygiP	b3060	0.38	+0.2
3,203,712	+	ygiP	b3060	0.39	+2.1
3,203,720	+	ygiP	b3060	0.40	+0.1
3,203,720	+	ygiP	b3060	0.40	-1.0
3,203,720	+	ygiP	b3060	0.40	+0.3
3,203,721	+	ygiP	b3060	0.40	+1.4
3,203,728	-	ygiP	b3060	0.41	+0.9
3,203,728	-	ygiP	b3060	0.41	+3.2
3,203,755	+	ygiP	b3060	0.44	-0.3
3,203,757	+	ygiP	b3060	0.44	-0.8
3,203,757	+	ygiP	b3060	0.44	-1.4
3,203,861	-	ygiP	b3060	0.55	-0.3
3,203,861	-	ygiP	b3060	0.55	-1.9
3,203,907	-	ygiP	b3060	0.60	+3.3
3,203,907	-	ygiP	b3060	0.60	-0.3
3,203,908	-	ygiP	b3060	0.60	+0.4
3,203,908	-	ygiP	b3060	0.60	+0.4
3,203,919	-	ygiP	b3060	0.61	+2.5
3,203,919	-	ygiP	b3060	0.61	-0.7
3,203,927	+	ygiP	b3060	0.62	+0.0
3,203,932	-	ygiP	b3060	0.63	+0.5
3,203,932	-	ygiP	b3060	0.63	+0.4
3,203,933	-	ygiP	b3060	0.63	-0.6
3,203,933	-	ygiP	b3060	0.63	+0.3
3,203,935	-	ygiP	b3060	0.63	+0.9
3,203,935	-	ygiP	b3060	0.63	-0.3
3,203,940	-	ygiP	b3060	0.64	+2.0
3,203,940	-	ygiP	b3060	0.64	-1.7
3,203,940	-	ygiP	b3060	0.64	-2.9
3,203,940	-	ygiP	b3060	0.64	-0.8
3,203,947	+	ygiP	b3060	0.64	+3.2
3,203,947	+	ygiP	b3060	0.64	+2.2
3,203,947	+	ygiP	b3060	0.64	+0.4
3,203,947	+	ygiP	b3060	0.64	+0.0
3,203,953	-	ygiP	b3060	0.65	+1.4
3,203,953	-	ygiP	b3060	0.65	-1.3
3,203,953	-	ygiP	b3060	0.65	+2.3
3,203,953	-	ygiP	b3060	0.65	-1.4
3,203,953	-	ygiP	b3060	0.65	+2.4
3,203,953	-	ygiP	b3060	0.65	-0.1
3,203,953	-	ygiP	b3060	0.65	+1.2
3,203,953	-	ygiP	b3060	0.65	+1.3
3,203,955	-	ygiP	b3060	0.65	+2.7
3,203,986	-	ygiP	b3060	0.69	-0.9
3,203,986	-	ygiP	b3060	0.69	+2.5
3,203,987	-	ygiP	b3060	0.69	-0.5
3,203,987	-	ygiP	b3060	0.69	+2.2
3,203,987	-	ygiP	b3060	0.69	-0.7
3,204,012	-	ygiP	b3060	0.71	+2.0
3,204,012	-	ygiP	b3060	0.71	+0.1
3,204,021	-	ygiP	b3060	0.72	-0.0
3,204,021	-	ygiP	b3060	0.72	-0.1
3,204,021	-	ygiP	b3060	0.72	+0.9
3,204,022	-	ygiP	b3060	0.72	+3.7
3,204,022	-	ygiP	b3060	0.72	+1.6
3,204,023	-	ygiP	b3060	0.73	+0.1
3,204,029	-	ygiP	b3060	0.73	-1.5
3,204,034	-	ygiP	b3060	0.74	-0.9
3,204,046	-	ygiP	b3060	0.75	+1.2
3,204,085	+	ygiP	b3060	0.79	-3.1
3,204,093	-	ygiP	b3060	0.80	-1.1
3,204,117	+	ygiP	b3060	0.83	-1.2
3,204,173	+	ygiP	b3060	0.89	-0.2
3,204,202	-				-0.3
3,204,202	-				-0.8
3,204,257	+				-1.6
3,204,263	-				-0.1
3,204,270	-				+1.6
3,204,275	-				-2.5
3,204,278	-				+0.0
3,204,294	+				+2.5
3,204,294	+				+1.6
3,204,294	+				+1.8
3,204,302	-				+0.1
3,204,302	-				+0.5
3,204,316	+				-0.1
3,204,319	-				-0.4
3,204,392	+				+3.2
3,204,394	-				-0.9
3,204,394	-				+1.5
3,204,490	-				-1.1
3,204,490	-				+2.5
3,204,506	-				+1.7
3,204,506	-				-0.3
3,204,525	-				+2.8
3,204,525	-				+2.3
3,204,530	+				-0.4
3,204,572	+				+1.8
3,204,572	+				-0.4
3,204,572	+				+2.3
3,204,573	+				+2.1
3,204,573	+				-2.6
3,204,579	-	ttdA	b3061	0.10	+1.7
3,204,579	-	ttdA	b3061	0.10	+2.2
3,204,580	-	ttdA	b3061	0.10	+0.1
3,204,580	-	ttdA	b3061	0.10	-0.3
3,204,581	-	ttdA	b3061	0.11	-3.3
3,204,581	-	ttdA	b3061	0.11	+2.3
3,204,584	+	ttdA	b3061	0.11	-2.1
3,204,586	+	ttdA	b3061	0.11	+1.7
3,204,591	+	ttdA	b3061	0.12	-1.4
3,204,615	-	ttdA	b3061	0.14	-0.5
3,204,615	-	ttdA	b3061	0.14	-1.0
3,204,619	+	ttdA	b3061	0.15	-0.1
3,204,642	+	ttdA	b3061	0.17	+1.0
3,204,650	-	ttdA	b3061	0.18	+2.7
3,204,668	-	ttdA	b3061	0.20	-1.5
3,204,668	-	ttdA	b3061	0.20	-0.1
3,204,668	-	ttdA	b3061	0.20	+0.5
3,204,685	-	ttdA	b3061	0.22	+0.6
3,204,685	-	ttdA	b3061	0.22	+0.2
3,204,685	-	ttdA	b3061	0.22	+2.9
3,204,685	-	ttdA	b3061	0.22	+2.1
3,204,710	-	ttdA	b3061	0.25	+3.0
3,204,721	-	ttdA	b3061	0.26	-1.8
3,204,852	-	ttdA	b3061	0.40	+2.1
3,204,852	-	ttdA	b3061	0.40	-0.2
3,204,852	-	ttdA	b3061	0.40	+1.9
3,204,852	-	ttdA	b3061	0.40	+0.7
3,204,888	+	ttdA	b3061	0.44	-0.6
3,204,895	-	ttdA	b3061	0.45	-0.6
3,204,895	-	ttdA	b3061	0.45	+1.2
3,204,896	-	ttdA	b3061	0.45	+0.0
3,204,953	+	ttdA	b3061	0.51	+2.6
3,204,982	-	ttdA	b3061	0.54	-1.9
3,205,061	+	ttdA	b3061	0.63	+1.3
3,205,185	+	ttdA	b3061	0.77	-1.7
3,205,185	+	ttdA	b3061	0.77	-0.9
3,205,222	-	ttdA	b3061	0.81	-0.3
3,205,252	-	ttdA	b3061	0.84	+1.7
3,205,252	-	ttdA	b3061	0.84	+1.5
3,205,293	+	ttdA	b3061	0.89	+0.7
3,205,296	+	ttdA	b3061	0.89	-0.4
3,205,296	+	ttdA	b3061	0.89	-0.9
3,205,345	-				+2.4
3,205,345	-				-1.4
3,205,372	+				+0.1
3,205,380	-				-0.3
3,205,401	-				+0.2
3,205,495	-	ttdB	b3062	0.17	-0.6
3,205,627	-	ttdB	b3062	0.39	-0.3
3,205,783	-	ttdB	b3062	0.64	-0.6
3,205,823	-	ttdB	b3062	0.71	+1.6
3,205,823	-	ttdB	b3062	0.71	+1.9
3,205,846	+	ttdB	b3062	0.75	+0.5
3,205,846	+	ttdB	b3062	0.75	-1.5
3,206,003	+				+1.5
3,206,011	-				-2.0
3,206,011	-				-1.1
3,206,046	+				+0.5
3,206,046	+				-0.9
3,206,068	+				+2.0
3,206,084	+				-0.4
3,206,155	-				+1.9
3,206,155	-				-0.8
3,206,233	-	ygjE	b3063	0.13	+0.9
3,206,241	+	ygjE	b3063	0.13	-1.3
3,206,251	+	ygjE	b3063	0.14	-0.6
3,206,259	-	ygjE	b3063	0.15	-0.3
3,206,264	+	ygjE	b3063	0.15	-0.0