Experiment: T7_phage 0.00015625 MOI TC4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygcQ and ygcR overlap by 4 nucleotides ygcR and ygcS overlap by 23 nucleotides ygcS and ygcU are separated by 93 nucleotides
b2769: ygcQ - putative flavoprotein (VIMSS), at 2,892,941 to 2,893,801
ygcQ
b2770: ygcR - putative transport protein (VIMSS), at 2,893,798 to 2,894,577
ygcR
b2771: ygcS - putative transport protein (VIMSS), at 2,894,555 to 2,895,892
ygcS
b4463: ygcU - predicted FAD containing dehydrogenase (NCBI), at 2,895,986 to 2,897,440
ygcU
Position (kb)
2894
2895
2896 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2893.566 kb on - strand, within ygcQ at 2893.636 kb on - strand, within ygcQ at 2893.657 kb on - strand, within ygcQ at 2893.717 kb on + strand at 2893.825 kb on + strand at 2893.825 kb on + strand at 2893.883 kb on - strand, within ygcR at 2893.891 kb on - strand, within ygcR at 2893.896 kb on - strand, within ygcR at 2893.959 kb on + strand, within ygcR at 2894.040 kb on + strand, within ygcR at 2894.040 kb on + strand, within ygcR at 2894.141 kb on - strand, within ygcR at 2894.260 kb on - strand, within ygcR at 2894.334 kb on - strand, within ygcR at 2894.334 kb on - strand, within ygcR at 2894.362 kb on + strand, within ygcR at 2894.372 kb on + strand, within ygcR at 2894.372 kb on + strand, within ygcR at 2894.373 kb on - strand, within ygcR at 2894.373 kb on - strand, within ygcR at 2894.500 kb on - strand at 2894.638 kb on + strand at 2894.756 kb on + strand, within ygcS at 2894.873 kb on + strand, within ygcS at 2894.873 kb on + strand, within ygcS at 2894.892 kb on + strand, within ygcS at 2894.999 kb on - strand, within ygcS at 2895.130 kb on - strand, within ygcS at 2895.187 kb on + strand, within ygcS at 2895.187 kb on + strand, within ygcS at 2895.442 kb on - strand, within ygcS at 2895.535 kb on - strand, within ygcS at 2895.559 kb on + strand, within ygcS at 2895.692 kb on - strand, within ygcS at 2895.721 kb on + strand, within ygcS at 2895.770 kb on + strand at 2895.803 kb on + strand at 2895.906 kb on - strand at 2896.019 kb on - strand at 2896.053 kb on + strand at 2896.053 kb on + strand at 2896.084 kb on - strand at 2896.170 kb on + strand, within ygcU at 2896.305 kb on - strand, within ygcU at 2896.357 kb on - strand, within ygcU at 2896.357 kb on - strand, within ygcU at 2896.423 kb on + strand, within ygcU at 2896.423 kb on + strand, within ygcU at 2896.531 kb on - strand, within ygcU at 2896.652 kb on - strand, within ygcU at 2896.732 kb on - strand, within ygcU at 2896.825 kb on - strand, within ygcU
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4 remove 2,893,566 - ygcQ b2769 0.73 +2.0 2,893,636 - ygcQ b2769 0.81 -0.1 2,893,657 - ygcQ b2769 0.83 +0.2 2,893,717 + -0.4 2,893,825 + -0.4 2,893,825 + -0.7 2,893,883 - ygcR b2770 0.11 -2.3 2,893,891 - ygcR b2770 0.12 +2.1 2,893,896 - ygcR b2770 0.13 -0.3 2,893,959 + ygcR b2770 0.21 +0.3 2,894,040 + ygcR b2770 0.31 -1.2 2,894,040 + ygcR b2770 0.31 +0.0 2,894,141 - ygcR b2770 0.44 +0.0 2,894,260 - ygcR b2770 0.59 -0.0 2,894,334 - ygcR b2770 0.69 +2.7 2,894,334 - ygcR b2770 0.69 -1.3 2,894,362 + ygcR b2770 0.72 +2.5 2,894,372 + ygcR b2770 0.74 +0.1 2,894,372 + ygcR b2770 0.74 -3.3 2,894,373 - ygcR b2770 0.74 +1.5 2,894,373 - ygcR b2770 0.74 +2.2 2,894,500 - +1.9 2,894,638 + -1.5 2,894,756 + ygcS b2771 0.15 -0.8 2,894,873 + ygcS b2771 0.24 -0.1 2,894,873 + ygcS b2771 0.24 -0.3 2,894,892 + ygcS b2771 0.25 -0.4 2,894,999 - ygcS b2771 0.33 -0.4 2,895,130 - ygcS b2771 0.43 -2.9 2,895,187 + ygcS b2771 0.47 +0.1 2,895,187 + ygcS b2771 0.47 -2.2 2,895,442 - ygcS b2771 0.66 -0.4 2,895,535 - ygcS b2771 0.73 +2.3 2,895,559 + ygcS b2771 0.75 +2.2 2,895,692 - ygcS b2771 0.85 -0.2 2,895,721 + ygcS b2771 0.87 +0.8 2,895,770 + -1.2 2,895,803 + -1.4 2,895,906 - +0.1 2,896,019 - -2.1 2,896,053 + +3.7 2,896,053 + +0.2 2,896,084 - +1.7 2,896,170 + ygcU b4463 0.13 -0.9 2,896,305 - ygcU b4463 0.22 -0.4 2,896,357 - ygcU b4463 0.25 -0.2 2,896,357 - ygcU b4463 0.25 -0.1 2,896,423 + ygcU b4463 0.30 -0.7 2,896,423 + ygcU b4463 0.30 -0.2 2,896,531 - ygcU b4463 0.37 -0.7 2,896,652 - ygcU b4463 0.46 +1.7 2,896,732 - ygcU b4463 0.51 +0.2 2,896,825 - ygcU b4463 0.58 -2.0
Or see this region's nucleotide sequence