Experiment: T7_phage 0.00015625 MOI TC4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfbL and yfbM are separated by 102 nucleotides yfbM and yfbN are separated by 272 nucleotides yfbN and yfbO are separated by 208 nucleotides yfbO and yfbP are separated by 58 nucleotides
b2271: yfbL - putative aminopeptidase (VIMSS), at 2,383,882 to 2,384,853
yfbL
b2272: yfbM - hypothetical protein (NCBI), at 2,384,956 to 2,385,459
yfbM
b2273: yfbN - hypothetical protein (NCBI), at 2,385,732 to 2,386,448
yfbN
b2274: yfbO - predicted protein (RefSeq), at 2,386,657 to 2,387,079
yfbO
b2275: yfbP - TPR-like repeats-containing protein (NCBI), at 2,387,138 to 2,387,986
yfbP
Position (kb)
2385
2386
2387 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2384.917 kb on - strand at 2385.135 kb on - strand, within yfbM at 2385.170 kb on - strand, within yfbM at 2385.184 kb on + strand, within yfbM at 2385.190 kb on - strand, within yfbM at 2385.202 kb on - strand, within yfbM at 2385.216 kb on + strand, within yfbM at 2385.252 kb on + strand, within yfbM at 2385.269 kb on + strand, within yfbM at 2385.269 kb on + strand, within yfbM at 2385.404 kb on + strand, within yfbM at 2385.420 kb on + strand at 2385.428 kb on + strand at 2385.441 kb on + strand at 2385.443 kb on + strand at 2385.444 kb on + strand at 2385.449 kb on + strand at 2385.456 kb on + strand at 2385.456 kb on + strand at 2385.458 kb on + strand at 2385.475 kb on + strand at 2385.476 kb on + strand at 2385.476 kb on + strand at 2385.484 kb on + strand at 2385.486 kb on + strand at 2385.553 kb on + strand at 2385.558 kb on + strand at 2385.558 kb on + strand at 2386.671 kb on - strand at 2386.721 kb on - strand, within yfbO at 2386.781 kb on - strand, within yfbO at 2386.781 kb on - strand, within yfbO at 2386.788 kb on - strand, within yfbO at 2386.819 kb on + strand, within yfbO at 2386.859 kb on - strand, within yfbO at 2386.859 kb on - strand, within yfbO at 2386.859 kb on - strand, within yfbO at 2386.870 kb on - strand, within yfbO at 2386.885 kb on - strand, within yfbO at 2386.898 kb on - strand, within yfbO at 2386.898 kb on - strand, within yfbO at 2386.908 kb on - strand, within yfbO at 2386.992 kb on - strand, within yfbO at 2386.992 kb on - strand, within yfbO at 2386.997 kb on + strand, within yfbO at 2387.005 kb on - strand, within yfbO at 2387.005 kb on - strand, within yfbO at 2387.013 kb on + strand, within yfbO at 2387.020 kb on + strand, within yfbO at 2387.020 kb on + strand, within yfbO at 2387.020 kb on + strand, within yfbO at 2387.020 kb on + strand, within yfbO at 2387.022 kb on + strand, within yfbO at 2387.041 kb on + strand at 2387.041 kb on + strand at 2387.044 kb on + strand at 2387.044 kb on + strand at 2387.046 kb on + strand at 2387.048 kb on + strand at 2387.048 kb on - strand at 2387.066 kb on - strand at 2387.214 kb on + strand at 2387.237 kb on + strand, within yfbP at 2387.245 kb on - strand, within yfbP at 2387.266 kb on + strand, within yfbP at 2387.266 kb on + strand, within yfbP at 2387.269 kb on - strand, within yfbP at 2387.280 kb on + strand, within yfbP at 2387.294 kb on - strand, within yfbP at 2387.297 kb on + strand, within yfbP at 2387.354 kb on + strand, within yfbP
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4 remove 2,384,917 - +2.8 2,385,135 - yfbM b2272 0.36 +0.8 2,385,170 - yfbM b2272 0.42 +1.8 2,385,184 + yfbM b2272 0.45 +3.4 2,385,190 - yfbM b2272 0.46 +2.8 2,385,202 - yfbM b2272 0.49 -0.1 2,385,216 + yfbM b2272 0.52 -0.7 2,385,252 + yfbM b2272 0.59 +1.7 2,385,269 + yfbM b2272 0.62 -0.5 2,385,269 + yfbM b2272 0.62 +1.7 2,385,404 + yfbM b2272 0.89 -0.2 2,385,420 + -2.5 2,385,428 + -0.9 2,385,441 + -1.5 2,385,443 + +1.9 2,385,444 + -1.9 2,385,449 + +0.1 2,385,456 + -1.5 2,385,456 + -1.0 2,385,458 + +1.7 2,385,475 + -1.2 2,385,476 + -1.3 2,385,476 + -0.5 2,385,484 + +3.5 2,385,486 + -0.6 2,385,553 + -0.2 2,385,558 + +1.8 2,385,558 + +2.8 2,386,671 - +2.7 2,386,721 - yfbO b2274 0.15 +2.7 2,386,781 - yfbO b2274 0.29 +1.2 2,386,781 - yfbO b2274 0.29 +0.2 2,386,788 - yfbO b2274 0.31 +4.7 2,386,819 + yfbO b2274 0.38 +2.7 2,386,859 - yfbO b2274 0.48 +0.6 2,386,859 - yfbO b2274 0.48 -0.6 2,386,859 - yfbO b2274 0.48 +1.2 2,386,870 - yfbO b2274 0.50 +2.6 2,386,885 - yfbO b2274 0.54 -0.4 2,386,898 - yfbO b2274 0.57 +0.4 2,386,898 - yfbO b2274 0.57 +0.7 2,386,908 - yfbO b2274 0.59 -0.4 2,386,992 - yfbO b2274 0.79 +2.7 2,386,992 - yfbO b2274 0.79 +0.7 2,386,997 + yfbO b2274 0.80 +0.2 2,387,005 - yfbO b2274 0.82 +2.7 2,387,005 - yfbO b2274 0.82 +1.7 2,387,013 + yfbO b2274 0.84 +0.7 2,387,020 + yfbO b2274 0.86 -2.5 2,387,020 + yfbO b2274 0.86 +0.4 2,387,020 + yfbO b2274 0.86 +1.7 2,387,020 + yfbO b2274 0.86 +2.7 2,387,022 + yfbO b2274 0.86 -0.0 2,387,041 + +0.4 2,387,041 + +1.2 2,387,044 + +1.1 2,387,044 + +0.0 2,387,046 + +1.8 2,387,048 + +1.6 2,387,048 - +1.4 2,387,066 - +2.7 2,387,214 + +2.7 2,387,237 + yfbP b2275 0.12 +0.2 2,387,245 - yfbP b2275 0.13 +0.4 2,387,266 + yfbP b2275 0.15 -1.9 2,387,266 + yfbP b2275 0.15 +0.2 2,387,269 - yfbP b2275 0.15 +0.8 2,387,280 + yfbP b2275 0.17 -1.6 2,387,294 - yfbP b2275 0.18 +1.2 2,387,297 + yfbP b2275 0.19 -1.6 2,387,354 + yfbP b2275 0.25 +2.0
Or see this region's nucleotide sequence