Strain Fitness in Escherichia coli BW25113 around b2212

Experiment: T7_phage 0.00015625 MOI TC4

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntyojI and alkB are separated by 75 nucleotidesalkB and ada overlap by 1 nucleotides b2211: yojI - fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 2,304,994 to 2,306,637 yojI b2212: alkB - oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI), at 2,306,713 to 2,307,363 alkB b2213: ada - fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI), at 2,307,363 to 2,308,427 ada Position (kb) 2306 2307 2308Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2305.720 kb on - strand, within yojIat 2305.720 kb on - strand, within yojIat 2305.747 kb on + strand, within yojIat 2305.747 kb on + strand, within yojIat 2305.760 kb on + strand, within yojIat 2305.763 kb on + strand, within yojIat 2305.799 kb on - strand, within yojIat 2305.829 kb on + strand, within yojIat 2305.848 kb on + strand, within yojIat 2305.890 kb on - strand, within yojIat 2305.935 kb on - strand, within yojIat 2306.040 kb on - strand, within yojIat 2306.078 kb on + strand, within yojIat 2306.139 kb on + strand, within yojIat 2306.139 kb on + strand, within yojIat 2306.337 kb on + strand, within yojIat 2306.337 kb on + strand, within yojIat 2306.433 kb on - strand, within yojIat 2306.444 kb on - strand, within yojIat 2306.444 kb on - strand, within yojIat 2306.444 kb on - strand, within yojIat 2306.500 kb on + strandat 2306.572 kb on + strandat 2306.572 kb on + strandat 2306.783 kb on + strand, within alkBat 2306.801 kb on - strand, within alkBat 2306.942 kb on - strand, within alkBat 2306.942 kb on - strand, within alkBat 2307.059 kb on + strand, within alkBat 2307.168 kb on - strand, within alkBat 2307.181 kb on - strand, within alkBat 2307.336 kb on + strandat 2307.336 kb on + strandat 2307.336 kb on + strandat 2307.368 kb on - strandat 2307.393 kb on + strandat 2307.549 kb on - strand, within adaat 2307.562 kb on - strand, within adaat 2307.583 kb on + strand, within adaat 2307.593 kb on + strand, within adaat 2307.849 kb on - strand, within adaat 2308.153 kb on + strand, within adaat 2308.153 kb on + strand, within adaat 2308.162 kb on + strand, within adaat 2308.327 kb on + strandat 2308.337 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4
remove
2,305,720 - yojI b2211 0.44 -3.6
2,305,720 - yojI b2211 0.44 -1.3
2,305,747 + yojI b2211 0.46 -0.7
2,305,747 + yojI b2211 0.46 -3.4
2,305,760 + yojI b2211 0.47 -0.3
2,305,763 + yojI b2211 0.47 +0.1
2,305,799 - yojI b2211 0.49 -1.8
2,305,829 + yojI b2211 0.51 -1.8
2,305,848 + yojI b2211 0.52 -2.7
2,305,890 - yojI b2211 0.55 -1.2
2,305,935 - yojI b2211 0.57 -1.2
2,306,040 - yojI b2211 0.64 -1.2
2,306,078 + yojI b2211 0.66 +2.4
2,306,139 + yojI b2211 0.70 -1.3
2,306,139 + yojI b2211 0.70 +0.6
2,306,337 + yojI b2211 0.82 -0.7
2,306,337 + yojI b2211 0.82 -0.3
2,306,433 - yojI b2211 0.88 +0.3
2,306,444 - yojI b2211 0.88 -0.8
2,306,444 - yojI b2211 0.88 -1.5
2,306,444 - yojI b2211 0.88 +0.1
2,306,500 + +2.6
2,306,572 + +0.1
2,306,572 + +0.2
2,306,783 + alkB b2212 0.11 -2.0
2,306,801 - alkB b2212 0.14 +2.0
2,306,942 - alkB b2212 0.35 -3.4
2,306,942 - alkB b2212 0.35 -0.1
2,307,059 + alkB b2212 0.53 -0.6
2,307,168 - alkB b2212 0.70 +0.1
2,307,181 - alkB b2212 0.72 -1.3
2,307,336 + +1.4
2,307,336 + -0.6
2,307,336 + -1.2
2,307,368 - -1.1
2,307,393 + -0.1
2,307,549 - ada b2213 0.17 -2.8
2,307,562 - ada b2213 0.19 -0.1
2,307,583 + ada b2213 0.21 -1.2
2,307,593 + ada b2213 0.22 -1.3
2,307,849 - ada b2213 0.46 -0.4
2,308,153 + ada b2213 0.74 -0.4
2,308,153 + ada b2213 0.74 -1.2
2,308,162 + ada b2213 0.75 -0.3
2,308,327 + +0.2
2,308,337 - -0.4

Or see this region's nucleotide sequence