Experiment: T7_phage 0.00015625 MOI TC4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insD-3 and insC overlap by 43 nucleotides insC and yeeP are separated by 479 nucleotides yeeP and isrC are separated by 103 nucleotides isrC and flu are separated by 20 nucleotides
b1996: insD-3 - CP4-44 prophage; IS2 insertion element transposase InsAB' (NCBI), at 2,066,976 to 2,067,881
insD-3
b1997: insC - KpLE2 phage-like element; IS2 insertion element repressor InsA (RefSeq), at 2,067,839 to 2,068,204
insC
b1999: yeeP - putative histone (VIMSS), at 2,068,684 to 2,069,235
yeeP
b4435: isrC - unknown RNA (NCBI), at 2,069,339 to 2,069,542
isrC
b2000: flu - CP4-44 prophage; antigen 43 (Ag43) phase-variable biofilm formation autotransporter (NCBI), at 2,069,563 to 2,072,682
flu
Position (kb)
2068
2069
2070 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2068.291 kb on + strand at 2068.344 kb on + strand at 2068.374 kb on + strand at 2068.384 kb on + strand at 2068.392 kb on - strand at 2068.392 kb on - strand at 2068.411 kb on + strand at 2068.428 kb on + strand at 2068.447 kb on - strand at 2068.447 kb on - strand at 2068.450 kb on - strand at 2068.545 kb on + strand at 2068.545 kb on + strand at 2068.566 kb on + strand at 2068.669 kb on - strand at 2068.672 kb on + strand at 2068.678 kb on + strand at 2068.698 kb on + strand at 2068.709 kb on + strand at 2068.775 kb on + strand, within yeeP at 2068.851 kb on + strand, within yeeP at 2068.851 kb on + strand, within yeeP at 2068.859 kb on - strand at 2068.859 kb on - strand, within yeeP at 2068.882 kb on - strand, within yeeP at 2069.072 kb on + strand, within yeeP at 2069.072 kb on + strand, within yeeP at 2069.212 kb on + strand at 2069.220 kb on - strand at 2069.396 kb on + strand, within isrC at 2069.396 kb on + strand, within isrC at 2069.541 kb on + strand at 2069.583 kb on - strand at 2069.877 kb on + strand, within flu at 2069.877 kb on + strand, within flu at 2069.936 kb on - strand, within flu at 2070.080 kb on + strand, within flu at 2070.101 kb on + strand, within flu at 2070.162 kb on + strand, within flu at 2070.170 kb on - strand, within flu
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4 remove 2,068,291 + +1.6 2,068,344 + +2.5 2,068,374 + +0.9 2,068,384 + -0.4 2,068,392 - +4.6 2,068,392 - -2.8 2,068,411 + +0.7 2,068,428 + +0.9 2,068,447 - -1.2 2,068,447 - -0.7 2,068,450 - +2.2 2,068,545 + +2.0 2,068,545 + +2.5 2,068,566 + +1.3 2,068,669 - +1.7 2,068,672 + +1.6 2,068,678 + +0.7 2,068,698 + +2.0 2,068,709 + +2.6 2,068,775 + yeeP b1999 0.16 +2.3 2,068,851 + yeeP b1999 0.30 +2.0 2,068,851 + yeeP b1999 0.30 +1.4 2,068,859 - -0.0 2,068,859 - yeeP b1999 0.32 +0.7 2,068,882 - yeeP b1999 0.36 -0.9 2,069,072 + yeeP b1999 0.70 +1.8 2,069,072 + yeeP b1999 0.70 +1.8 2,069,212 + +2.2 2,069,220 - +2.6 2,069,396 + isrC b4435 0.28 +1.2 2,069,396 + isrC b4435 0.28 +2.4 2,069,541 + +0.3 2,069,583 - -0.5 2,069,877 + flu b2000 0.10 -1.4 2,069,877 + flu b2000 0.10 -1.0 2,069,936 - flu b2000 0.12 -0.5 2,070,080 + flu b2000 0.17 -0.7 2,070,101 + flu b2000 0.17 +0.2 2,070,162 + flu b2000 0.19 -1.0 2,070,170 - flu b2000 0.19 -2.1
Or see this region's nucleotide sequence