Strain Fitness in Escherichia coli BW25113 around b1999

Experiment: T7_phage 0.00015625 MOI TC4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsD-3 and insC overlap by 43 nucleotidesinsC and yeeP are separated by 479 nucleotidesyeeP and isrC are separated by 103 nucleotidesisrC and flu are separated by 20 nucleotides b1996: insD-3 - CP4-44 prophage; IS2 insertion element transposase InsAB' (NCBI), at 2,066,976 to 2,067,881 insD-3 b1997: insC - KpLE2 phage-like element; IS2 insertion element repressor InsA (RefSeq), at 2,067,839 to 2,068,204 insC b1999: yeeP - putative histone (VIMSS), at 2,068,684 to 2,069,235 yeeP b4435: isrC - unknown RNA (NCBI), at 2,069,339 to 2,069,542 isrC b2000: flu - CP4-44 prophage; antigen 43 (Ag43) phase-variable biofilm formation autotransporter (NCBI), at 2,069,563 to 2,072,682 flu Position (kb) 2068 2069 2070Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2068.291 kb on + strandat 2068.344 kb on + strandat 2068.374 kb on + strandat 2068.384 kb on + strandat 2068.392 kb on - strandat 2068.392 kb on - strandat 2068.411 kb on + strandat 2068.428 kb on + strandat 2068.447 kb on - strandat 2068.447 kb on - strandat 2068.450 kb on - strandat 2068.545 kb on + strandat 2068.545 kb on + strandat 2068.566 kb on + strandat 2068.669 kb on - strandat 2068.672 kb on + strandat 2068.678 kb on + strandat 2068.698 kb on + strandat 2068.709 kb on + strandat 2068.775 kb on + strand, within yeePat 2068.851 kb on + strand, within yeePat 2068.851 kb on + strand, within yeePat 2068.859 kb on - strandat 2068.859 kb on - strand, within yeePat 2068.882 kb on - strand, within yeePat 2069.072 kb on + strand, within yeePat 2069.072 kb on + strand, within yeePat 2069.212 kb on + strandat 2069.220 kb on - strandat 2069.396 kb on + strand, within isrCat 2069.396 kb on + strand, within isrCat 2069.541 kb on + strandat 2069.583 kb on - strandat 2069.877 kb on + strand, within fluat 2069.877 kb on + strand, within fluat 2069.936 kb on - strand, within fluat 2070.080 kb on + strand, within fluat 2070.101 kb on + strand, within fluat 2070.162 kb on + strand, within fluat 2070.170 kb on - strand, within flu

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4
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2,068,291 + +1.6
2,068,344 + +2.5
2,068,374 + +0.9
2,068,384 + -0.4
2,068,392 - +4.6
2,068,392 - -2.8
2,068,411 + +0.7
2,068,428 + +0.9
2,068,447 - -1.2
2,068,447 - -0.7
2,068,450 - +2.2
2,068,545 + +2.0
2,068,545 + +2.5
2,068,566 + +1.3
2,068,669 - +1.7
2,068,672 + +1.6
2,068,678 + +0.7
2,068,698 + +2.0
2,068,709 + +2.6
2,068,775 + yeeP b1999 0.16 +2.3
2,068,851 + yeeP b1999 0.30 +2.0
2,068,851 + yeeP b1999 0.30 +1.4
2,068,859 - -0.0
2,068,859 - yeeP b1999 0.32 +0.7
2,068,882 - yeeP b1999 0.36 -0.9
2,069,072 + yeeP b1999 0.70 +1.8
2,069,072 + yeeP b1999 0.70 +1.8
2,069,212 + +2.2
2,069,220 - +2.6
2,069,396 + isrC b4435 0.28 +1.2
2,069,396 + isrC b4435 0.28 +2.4
2,069,541 + +0.3
2,069,583 - -0.5
2,069,877 + flu b2000 0.10 -1.4
2,069,877 + flu b2000 0.10 -1.0
2,069,936 - flu b2000 0.12 -0.5
2,070,080 + flu b2000 0.17 -0.7
2,070,101 + flu b2000 0.17 +0.2
2,070,162 + flu b2000 0.19 -1.0
2,070,170 - flu b2000 0.19 -2.1

Or see this region's nucleotide sequence