Strain Fitness in Escherichia coli BW25113 around b1622

Experiment: T7_phage 0.00015625 MOI TC4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmalX and malY are separated by 9 nucleotidesmalY and add are separated by 103 nucleotides b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971 malX b1622: malY - bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI), at 1,698,981 to 1,700,153 malY b1623: add - adenosine deaminase (NCBI), at 1,700,257 to 1,701,258 add Position (kb) 1698 1699 1700 1701Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1698.219 kb on - strand, within malXat 1698.303 kb on + strand, within malXat 1698.450 kb on + strand, within malXat 1698.450 kb on + strand, within malXat 1698.495 kb on + strand, within malXat 1698.716 kb on - strand, within malXat 1698.730 kb on - strand, within malXat 1698.918 kb on - strandat 1698.950 kb on + strandat 1698.950 kb on + strandat 1698.952 kb on + strandat 1699.029 kb on - strandat 1699.060 kb on + strandat 1699.068 kb on - strandat 1699.068 kb on - strandat 1699.152 kb on + strand, within malYat 1699.279 kb on + strand, within malYat 1699.279 kb on + strand, within malYat 1699.546 kb on + strand, within malYat 1699.546 kb on + strand, within malYat 1699.566 kb on - strand, within malYat 1699.796 kb on + strand, within malYat 1700.152 kb on + strandat 1700.214 kb on + strandat 1700.313 kb on - strandat 1700.327 kb on + strandat 1700.572 kb on - strand, within addat 1700.727 kb on + strand, within addat 1700.929 kb on - strand, within addat 1701.020 kb on + strand, within addat 1701.061 kb on - strand, within addat 1701.086 kb on - strand, within addat 1701.086 kb on - strand, within addat 1701.146 kb on + strand, within add

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4
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1,698,219 - malX b1621 0.53 +0.9
1,698,303 + malX b1621 0.58 +0.0
1,698,450 + malX b1621 0.67 -1.0
1,698,450 + malX b1621 0.67 -0.8
1,698,495 + malX b1621 0.70 +2.0
1,698,716 - malX b1621 0.84 -1.3
1,698,730 - malX b1621 0.85 -1.1
1,698,918 - -0.8
1,698,950 + -0.2
1,698,950 + -0.9
1,698,952 + -1.2
1,699,029 - +1.1
1,699,060 + +1.3
1,699,068 - -1.1
1,699,068 - -0.9
1,699,152 + malY b1622 0.15 -0.5
1,699,279 + malY b1622 0.25 +1.1
1,699,279 + malY b1622 0.25 +0.4
1,699,546 + malY b1622 0.48 -0.8
1,699,546 + malY b1622 0.48 -0.9
1,699,566 - malY b1622 0.50 -0.5
1,699,796 + malY b1622 0.69 -1.0
1,700,152 + -0.2
1,700,214 + -0.4
1,700,313 - -2.3
1,700,327 + +1.7
1,700,572 - add b1623 0.31 +1.7
1,700,727 + add b1623 0.47 -1.8
1,700,929 - add b1623 0.67 +1.1
1,701,020 + add b1623 0.76 -0.5
1,701,061 - add b1623 0.80 -3.1
1,701,086 - add b1623 0.83 -0.4
1,701,086 - add b1623 0.83 -0.9
1,701,146 + add b1623 0.89 -0.5

Or see this region's nucleotide sequence