Strain Fitness in Escherichia coli BW25113 around b1507

Experiment: T7_phage 0.00015625 MOI TC4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydeT and yneL are separated by 254 nucleotidesyneL and hipA are separated by 317 nucleotideshipA and hipB overlap by 1 nucleotideshipB and yneO are separated by 222 nucleotides b1505: ydeT - hypothetical protein (RefSeq), at 1,586,877 to 1,588,103 ydeT b1506: yneL - predicted transcriptional regulator (NCBI), at 1,588,358 to 1,588,560 yneL b1507: hipA - regulator with hipB (NCBI), at 1,588,878 to 1,590,200 hipA b1508: hipB - DNA-binding transcriptional regulator (NCBI), at 1,590,200 to 1,590,466 hipB b4696: yneO - no description, at 1,590,689 to 1,596,011 yneO Position (kb) 1588 1589 1590 1591Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1588.339 kb on - strandat 1588.354 kb on - strandat 1588.406 kb on - strand, within yneLat 1588.460 kb on - strand, within yneLat 1588.474 kb on - strand, within yneLat 1588.483 kb on - strand, within yneLat 1588.483 kb on - strand, within yneLat 1588.494 kb on - strand, within yneLat 1588.997 kb on - strandat 1589.129 kb on + strand, within hipAat 1589.163 kb on - strand, within hipAat 1589.222 kb on + strand, within hipAat 1589.337 kb on - strand, within hipAat 1589.584 kb on + strand, within hipAat 1589.584 kb on + strand, within hipAat 1589.592 kb on - strand, within hipAat 1589.592 kb on - strand, within hipAat 1589.629 kb on - strand, within hipAat 1589.888 kb on - strand, within hipAat 1589.977 kb on + strand, within hipAat 1590.648 kb on + strandat 1590.772 kb on + strandat 1590.807 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4
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1,588,339 - +0.6
1,588,354 - +0.9
1,588,406 - yneL b1506 0.24 -0.4
1,588,460 - yneL b1506 0.50 +3.0
1,588,474 - yneL b1506 0.57 +0.4
1,588,483 - yneL b1506 0.62 -0.1
1,588,483 - yneL b1506 0.62 -0.4
1,588,494 - yneL b1506 0.67 +3.2
1,588,997 - +0.1
1,589,129 + hipA b1507 0.19 +2.4
1,589,163 - hipA b1507 0.22 -0.1
1,589,222 + hipA b1507 0.26 +2.4
1,589,337 - hipA b1507 0.35 -0.8
1,589,584 + hipA b1507 0.53 -1.3
1,589,584 + hipA b1507 0.53 +3.4
1,589,592 - hipA b1507 0.54 +3.5
1,589,592 - hipA b1507 0.54 +3.1
1,589,629 - hipA b1507 0.57 -2.1
1,589,888 - hipA b1507 0.76 -0.6
1,589,977 + hipA b1507 0.83 +2.6
1,590,648 + -2.2
1,590,772 + +0.2
1,590,807 + -0.9

Or see this region's nucleotide sequence