Experiment: T7_phage 0.00015625 MOI TC4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hepA and polB are separated by 164 nucleotides polB and araD are separated by 74 nucleotides
b0059: hepA - ATP-dependent helicase HepA (NCBI), at 60,358 to 63,264
hepA
b0060: polB - DNA polymerase II (NCBI), at 63,429 to 65,780
polB
b0061: araD - L-ribulose-5-phosphate 4-epimerase (NCBI), at 65,855 to 66,550
araD
Position (kb) 63
64
65
66 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 62.440 kb on + strand, within hepA at 62.541 kb on - strand, within hepA at 62.541 kb on - strand, within hepA at 62.545 kb on + strand, within hepA at 62.558 kb on + strand, within hepA at 62.558 kb on + strand, within hepA at 62.558 kb on + strand, within hepA at 62.566 kb on - strand, within hepA at 62.570 kb on + strand, within hepA at 62.578 kb on - strand, within hepA at 62.625 kb on + strand, within hepA at 62.625 kb on + strand, within hepA at 62.626 kb on - strand, within hepA at 62.631 kb on - strand, within hepA at 62.694 kb on + strand, within hepA at 62.810 kb on + strand, within hepA at 62.810 kb on + strand, within hepA at 62.810 kb on + strand, within hepA at 62.813 kb on - strand, within hepA at 62.926 kb on + strand, within hepA at 62.934 kb on - strand, within hepA at 62.976 kb on + strand at 62.976 kb on + strand at 62.981 kb on + strand at 63.103 kb on + strand at 63.260 kb on - strand at 63.318 kb on + strand at 63.324 kb on - strand at 63.451 kb on + strand at 63.524 kb on + strand at 63.528 kb on - strand at 63.528 kb on - strand at 63.532 kb on + strand at 63.545 kb on - strand at 63.577 kb on + strand at 63.577 kb on + strand at 63.627 kb on + strand at 63.683 kb on + strand, within polB at 63.727 kb on + strand, within polB at 63.821 kb on + strand, within polB at 63.821 kb on + strand, within polB at 63.830 kb on - strand, within polB at 63.920 kb on - strand, within polB at 63.948 kb on + strand, within polB at 63.994 kb on + strand, within polB at 63.994 kb on + strand, within polB at 64.007 kb on + strand, within polB at 64.120 kb on - strand, within polB at 64.269 kb on + strand, within polB at 64.269 kb on + strand, within polB at 64.281 kb on + strand, within polB at 64.332 kb on - strand, within polB at 64.332 kb on - strand, within polB at 64.340 kb on + strand, within polB at 64.429 kb on + strand, within polB at 64.436 kb on + strand, within polB at 64.436 kb on + strand, within polB at 64.500 kb on - strand, within polB at 64.514 kb on + strand, within polB at 64.540 kb on + strand, within polB at 64.568 kb on + strand, within polB at 65.051 kb on + strand, within polB at 65.172 kb on - strand, within polB at 65.190 kb on + strand, within polB at 65.190 kb on + strand, within polB at 65.278 kb on + strand, within polB at 65.278 kb on + strand, within polB at 65.286 kb on - strand, within polB at 65.291 kb on - strand, within polB at 65.291 kb on - strand, within polB at 65.315 kb on + strand, within polB at 65.317 kb on + strand, within polB at 65.334 kb on - strand, within polB at 65.398 kb on + strand, within polB at 65.406 kb on - strand, within polB at 65.411 kb on + strand, within polB at 65.427 kb on - strand, within polB at 65.482 kb on - strand, within polB at 65.486 kb on - strand, within polB at 65.493 kb on - strand, within polB at 65.493 kb on - strand, within polB at 65.541 kb on + strand, within polB at 65.541 kb on + strand, within polB at 65.652 kb on - strand at 65.692 kb on - strand at 65.814 kb on - strand at 65.816 kb on + strand at 65.824 kb on - strand at 65.865 kb on + strand at 65.873 kb on - strand at 65.873 kb on - strand at 65.883 kb on + strand at 65.883 kb on + strand at 65.891 kb on - strand at 65.900 kb on - strand at 65.949 kb on + strand, within araD at 65.949 kb on + strand, within araD at 65.977 kb on + strand, within araD at 66.057 kb on - strand, within araD at 66.057 kb on - strand, within araD at 66.195 kb on + strand, within araD at 66.226 kb on + strand, within araD at 66.226 kb on + strand, within araD at 66.271 kb on - strand, within araD at 66.271 kb on - strand, within araD at 66.276 kb on - strand, within araD at 66.381 kb on + strand, within araD at 66.381 kb on + strand, within araD at 66.470 kb on + strand, within araD at 66.470 kb on + strand, within araD at 66.473 kb on - strand, within araD at 66.473 kb on - strand, within araD at 66.483 kb on - strand at 66.484 kb on + strand at 66.484 kb on + strand at 66.527 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC4 remove 62,440 + hepA b0059 0.72 +0.0 62,541 - hepA b0059 0.75 -1.6 62,541 - hepA b0059 0.75 -1.6 62,545 + hepA b0059 0.75 -0.7 62,558 + hepA b0059 0.76 +3.5 62,558 + hepA b0059 0.76 +0.7 62,558 + hepA b0059 0.76 +3.0 62,566 - hepA b0059 0.76 -0.3 62,570 + hepA b0059 0.76 +0.0 62,578 - hepA b0059 0.76 +1.5 62,625 + hepA b0059 0.78 +2.4 62,625 + hepA b0059 0.78 +2.9 62,626 - hepA b0059 0.78 +3.0 62,631 - hepA b0059 0.78 -0.3 62,694 + hepA b0059 0.80 -0.1 62,810 + hepA b0059 0.84 -0.9 62,810 + hepA b0059 0.84 -0.5 62,810 + hepA b0059 0.84 +0.1 62,813 - hepA b0059 0.84 +3.1 62,926 + hepA b0059 0.88 +2.1 62,934 - hepA b0059 0.89 +0.3 62,976 + +0.3 62,976 + +0.1 62,981 + -0.2 63,103 + -0.4 63,260 - +0.5 63,318 + +1.8 63,324 - +2.0 63,451 + +1.1 63,524 + -0.4 63,528 - -0.8 63,528 - -1.3 63,532 + -1.8 63,545 - -0.2 63,577 + +0.1 63,577 + -0.9 63,627 + -0.2 63,683 + polB b0060 0.11 +0.0 63,727 + polB b0060 0.13 -1.7 63,821 + polB b0060 0.17 +0.8 63,821 + polB b0060 0.17 +2.3 63,830 - polB b0060 0.17 -1.7 63,920 - polB b0060 0.21 -1.1 63,948 + polB b0060 0.22 +2.3 63,994 + polB b0060 0.24 -3.0 63,994 + polB b0060 0.24 -0.6 64,007 + polB b0060 0.25 +2.3 64,120 - polB b0060 0.29 -1.3 64,269 + polB b0060 0.36 -0.1 64,269 + polB b0060 0.36 -0.7 64,281 + polB b0060 0.36 -0.6 64,332 - polB b0060 0.38 -0.3 64,332 - polB b0060 0.38 -0.9 64,340 + polB b0060 0.39 -0.3 64,429 + polB b0060 0.43 -0.6 64,436 + polB b0060 0.43 -1.6 64,436 + polB b0060 0.43 -0.4 64,500 - polB b0060 0.46 -0.8 64,514 + polB b0060 0.46 -0.5 64,540 + polB b0060 0.47 -2.1 64,568 + polB b0060 0.48 -0.2 65,051 + polB b0060 0.69 -1.7 65,172 - polB b0060 0.74 -0.1 65,190 + polB b0060 0.75 -0.9 65,190 + polB b0060 0.75 +2.5 65,278 + polB b0060 0.79 -3.9 65,278 + polB b0060 0.79 +1.7 65,286 - polB b0060 0.79 -2.7 65,291 - polB b0060 0.79 -0.1 65,291 - polB b0060 0.79 -0.1 65,315 + polB b0060 0.80 -0.1 65,317 + polB b0060 0.80 +1.5 65,334 - polB b0060 0.81 +0.6 65,398 + polB b0060 0.84 +1.3 65,406 - polB b0060 0.84 -0.0 65,411 + polB b0060 0.84 -1.1 65,427 - polB b0060 0.85 -2.5 65,482 - polB b0060 0.87 +2.5 65,486 - polB b0060 0.87 -0.4 65,493 - polB b0060 0.88 -0.5 65,493 - polB b0060 0.88 -0.0 65,541 + polB b0060 0.90 -1.2 65,541 + polB b0060 0.90 +1.8 65,652 - +0.5 65,692 - +1.6 65,814 - +2.2 65,816 + +0.0 65,824 - -0.5 65,865 + -1.5 65,873 - +1.0 65,873 - +2.2 65,883 + -0.3 65,883 + -0.0 65,891 - +1.6 65,900 - -0.8 65,949 + araD b0061 0.14 -1.3 65,949 + araD b0061 0.14 -1.5 65,977 + araD b0061 0.18 +2.0 66,057 - araD b0061 0.29 +0.5 66,057 - araD b0061 0.29 +0.6 66,195 + araD b0061 0.49 +2.1 66,226 + araD b0061 0.53 -1.1 66,226 + araD b0061 0.53 -0.3 66,271 - araD b0061 0.60 -3.1 66,271 - araD b0061 0.60 -0.7 66,276 - araD b0061 0.60 -1.6 66,381 + araD b0061 0.76 -1.1 66,381 + araD b0061 0.76 +0.4 66,470 + araD b0061 0.88 -1.9 66,470 + araD b0061 0.88 -0.5 66,473 - araD b0061 0.89 +0.9 66,473 - araD b0061 0.89 -0.9 66,483 - -1.8 66,484 + +0.7 66,484 + -1.2 66,527 - +2.4
Or see this region's nucleotide sequence