Strain Fitness in Escherichia coli BW25113 around b4035

Experiment: T7_phage 0.000015625 MOI TC3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmalE and malK are separated by 364 nucleotidesmalK and lamB are separated by 71 nucleotides b4034: malE - maltose ABC transporter periplasmic protein (NCBI), at 4,243,252 to 4,244,442 malE b4035: malK - fused maltose transport subunit, ATP-binding component of ABC superfamily/regulatory protein (NCBI), at 4,244,807 to 4,245,922 malK b4036: lamB - maltoporin precursor (NCBI), at 4,245,994 to 4,247,334 lamB Position (kb) 4244 4245 4246Strain fitness (log2 ratio) -2 -1 0 1at 4243.808 kb on + strand, within malEat 4243.939 kb on - strand, within malEat 4243.952 kb on + strand, within malEat 4243.957 kb on + strand, within malEat 4244.016 kb on + strand, within malEat 4244.046 kb on + strand, within malEat 4244.050 kb on + strand, within malEat 4244.058 kb on + strand, within malEat 4244.112 kb on - strand, within malEat 4244.154 kb on - strand, within malEat 4244.154 kb on - strand, within malEat 4244.174 kb on + strand, within malEat 4244.174 kb on + strand, within malEat 4244.185 kb on + strand, within malEat 4244.198 kb on - strand, within malEat 4244.210 kb on + strand, within malEat 4244.417 kb on - strandat 4244.466 kb on - strandat 4244.491 kb on + strandat 4244.491 kb on + strandat 4244.537 kb on + strandat 4244.537 kb on + strandat 4244.809 kb on + strandat 4244.840 kb on + strandat 4244.925 kb on - strand, within malKat 4244.925 kb on - strand, within malKat 4244.960 kb on + strand, within malKat 4245.007 kb on + strand, within malKat 4245.018 kb on + strand, within malKat 4245.154 kb on + strand, within malKat 4245.154 kb on + strand, within malKat 4245.162 kb on - strand, within malKat 4245.228 kb on - strand, within malKat 4245.240 kb on + strand, within malKat 4245.240 kb on + strand, within malKat 4245.289 kb on + strand, within malKat 4245.308 kb on - strand, within malKat 4245.566 kb on - strand, within malKat 4245.799 kb on + strand, within malKat 4245.822 kb on - strandat 4245.882 kb on + strandat 4245.884 kb on + strandat 4245.884 kb on + strandat 4245.948 kb on + strandat 4245.956 kb on - strandat 4245.989 kb on + strandat 4246.115 kb on - strandat 4246.164 kb on - strand, within lamBat 4246.164 kb on - strand, within lamBat 4246.271 kb on - strand, within lamBat 4246.271 kb on - strand, within lamBat 4246.425 kb on - strand, within lamBat 4246.433 kb on + strand, within lamBat 4246.476 kb on + strand, within lamBat 4246.477 kb on + strand, within lamBat 4246.505 kb on + strand, within lamBat 4246.546 kb on + strand, within lamBat 4246.546 kb on + strand, within lamBat 4246.629 kb on + strand, within lamBat 4246.639 kb on - strand, within lamBat 4246.689 kb on - strand, within lamBat 4246.715 kb on + strand, within lamBat 4246.715 kb on + strand, within lamBat 4246.727 kb on + strand, within lamBat 4246.733 kb on - strand, within lamBat 4246.794 kb on + strand, within lamBat 4246.794 kb on + strand, within lamBat 4246.794 kb on + strand, within lamBat 4246.878 kb on + strand, within lamBat 4246.878 kb on + strand, within lamBat 4246.895 kb on + strand, within lamBat 4246.895 kb on + strand, within lamBat 4246.904 kb on + strand, within lamB

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.000015625 MOI TC3
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4,243,808 + malE b4034 0.47 -0.4
4,243,939 - malE b4034 0.58 +0.4
4,243,952 + malE b4034 0.59 +0.3
4,243,957 + malE b4034 0.59 +0.1
4,244,016 + malE b4034 0.64 +0.7
4,244,046 + malE b4034 0.67 +0.1
4,244,050 + malE b4034 0.67 +0.7
4,244,058 + malE b4034 0.68 -0.3
4,244,112 - malE b4034 0.72 -0.4
4,244,154 - malE b4034 0.76 +0.6
4,244,154 - malE b4034 0.76 +0.6
4,244,174 + malE b4034 0.77 -0.8
4,244,174 + malE b4034 0.77 -0.3
4,244,185 + malE b4034 0.78 +0.2
4,244,198 - malE b4034 0.79 -0.2
4,244,210 + malE b4034 0.80 -1.2
4,244,417 - -0.8
4,244,466 - -0.1
4,244,491 + -1.4
4,244,491 + +1.1
4,244,537 + -0.6
4,244,537 + +0.5
4,244,809 + +0.7
4,244,840 + -1.5
4,244,925 - malK b4035 0.11 -1.7
4,244,925 - malK b4035 0.11 -1.6
4,244,960 + malK b4035 0.14 -0.9
4,245,007 + malK b4035 0.18 -1.7
4,245,018 + malK b4035 0.19 -1.2
4,245,154 + malK b4035 0.31 -0.7
4,245,154 + malK b4035 0.31 -1.4
4,245,162 - malK b4035 0.32 -0.9
4,245,228 - malK b4035 0.38 -0.1
4,245,240 + malK b4035 0.39 -1.3
4,245,240 + malK b4035 0.39 -0.4
4,245,289 + malK b4035 0.43 -0.3
4,245,308 - malK b4035 0.45 -1.7
4,245,566 - malK b4035 0.68 -1.3
4,245,799 + malK b4035 0.89 -0.1
4,245,822 - -0.7
4,245,882 + +0.3
4,245,884 + -0.6
4,245,884 + -0.2
4,245,948 + -0.2
4,245,956 - -0.5
4,245,989 + +0.1
4,246,115 - +0.9
4,246,164 - lamB b4036 0.13 +0.2
4,246,164 - lamB b4036 0.13 +0.3
4,246,271 - lamB b4036 0.21 -0.5
4,246,271 - lamB b4036 0.21 +1.0
4,246,425 - lamB b4036 0.32 -0.9
4,246,433 + lamB b4036 0.33 -0.3
4,246,476 + lamB b4036 0.36 -1.9
4,246,477 + lamB b4036 0.36 -0.4
4,246,505 + lamB b4036 0.38 -1.0
4,246,546 + lamB b4036 0.41 -0.7
4,246,546 + lamB b4036 0.41 +0.1
4,246,629 + lamB b4036 0.47 +0.1
4,246,639 - lamB b4036 0.48 +0.3
4,246,689 - lamB b4036 0.52 +0.8
4,246,715 + lamB b4036 0.54 +0.6
4,246,715 + lamB b4036 0.54 -0.2
4,246,727 + lamB b4036 0.55 -1.4
4,246,733 - lamB b4036 0.55 +0.4
4,246,794 + lamB b4036 0.60 -0.1
4,246,794 + lamB b4036 0.60 +0.1
4,246,794 + lamB b4036 0.60 -0.6
4,246,878 + lamB b4036 0.66 -0.1
4,246,878 + lamB b4036 0.66 +0.1
4,246,895 + lamB b4036 0.67 +0.0
4,246,895 + lamB b4036 0.67 -1.5
4,246,904 + lamB b4036 0.68 -0.3

Or see this region's nucleotide sequence