Strain Fitness in Escherichia coli BW25113 around b3947

Experiment: T7_phage 0.00015625 MOI TC3

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	T7_phage 0.00015625 MOI TC3
4,136,784	+	gldA	b3945	0.75	-1.6
4,136,784	+	gldA	b3945	0.75	+1.2
4,136,812	-	gldA	b3945	0.78	-2.1
4,136,812	-	gldA	b3945	0.78	+1.4
4,136,916	+				-0.2
4,136,970	+				+0.7
4,136,970	+				+0.5
4,137,030	-				+1.8
4,137,061	-				-0.1
4,137,061	-				+2.2
4,137,105	-				-1.5
4,137,105	-				-2.9
4,137,105	-				-1.4
4,137,196	+	fsaB	b3946	0.19	-2.1
4,137,214	+	fsaB	b3946	0.22	+0.5
4,137,215	-	fsaB	b3946	0.22	-3.4
4,137,234	-	fsaB	b3946	0.25	-1.2
4,137,288	+	fsaB	b3946	0.33	-0.7
4,137,288	+	fsaB	b3946	0.33	-0.0
4,137,288	+	fsaB	b3946	0.33	-3.5
4,137,291	-	fsaB	b3946	0.33	-3.0
4,137,296	-	fsaB	b3946	0.34	-2.9
4,137,296	-	fsaB	b3946	0.34	-0.7
4,137,344	-	fsaB	b3946	0.41	+0.5
4,137,389	-	fsaB	b3946	0.48	-2.1
4,137,389	-	fsaB	b3946	0.48	-2.7
4,137,393	+	fsaB	b3946	0.49	-4.0
4,137,396	+	fsaB	b3946	0.49	-0.5
4,137,401	-	fsaB	b3946	0.50	-0.9
4,137,401	-	fsaB	b3946	0.50	-1.6
4,137,404	-	fsaB	b3946	0.51	+0.9
4,137,404	-	fsaB	b3946	0.51	-0.5
4,137,404	-	fsaB	b3946	0.51	+0.4
4,137,404	-	fsaB	b3946	0.51	-0.7
4,137,468	-	fsaB	b3946	0.60	-3.0
4,137,507	+	fsaB	b3946	0.66	-0.5
4,137,525	-	fsaB	b3946	0.69	+0.4
4,137,525	-	fsaB	b3946	0.69	+1.4
4,137,539	-	fsaB	b3946	0.71	-0.6
4,137,539	-	fsaB	b3946	0.71	+0.3
4,137,552	+	fsaB	b3946	0.73	-0.8
4,137,610	+	fsaB	b3946	0.82	-0.6
4,137,647	+	fsaB	b3946	0.87	-1.7
4,137,703	+				-1.0
4,137,703	-				+1.1
4,137,705	-				-0.0
4,137,866	-				+1.9
4,137,866	-				-2.7
4,137,900	-				+1.2
4,137,900	-				-1.8
4,137,900	-				+2.2
4,138,103	-	ptsA	b3947	0.14	+0.1
4,138,103	-	ptsA	b3947	0.14	+1.6
4,138,112	-	ptsA	b3947	0.15	-1.5
4,138,124	-	ptsA	b3947	0.15	-0.5
4,138,205	-	ptsA	b3947	0.18	-1.8
4,138,205	-	ptsA	b3947	0.18	-2.9
4,138,208	-	ptsA	b3947	0.19	-0.5
4,138,259	+	ptsA	b3947	0.21	+0.8
4,138,351	+	ptsA	b3947	0.24	-3.4
4,138,396	+	ptsA	b3947	0.26	-0.4
4,138,398	+	ptsA	b3947	0.26	-2.1
4,138,398	+	ptsA	b3947	0.26	-3.4
4,138,444	-	ptsA	b3947	0.28	-2.9
4,138,444	-	ptsA	b3947	0.28	+0.7
4,138,446	+	ptsA	b3947	0.28	+0.9
4,138,459	+	ptsA	b3947	0.29	+1.7
4,138,467	-	ptsA	b3947	0.29	+0.8
4,138,662	-	ptsA	b3947	0.37	+0.2
4,138,705	+	ptsA	b3947	0.38	-1.4
4,138,705	+	ptsA	b3947	0.38	-0.0
4,138,752	+	ptsA	b3947	0.40	-0.1
4,138,787	+	ptsA	b3947	0.42	+0.9
4,138,787	+	ptsA	b3947	0.42	+0.2
4,138,792	+	ptsA	b3947	0.42	-0.2
4,138,797	+	ptsA	b3947	0.42	-0.9
4,138,823	+	ptsA	b3947	0.43	-0.8
4,138,926	-	ptsA	b3947	0.47	+0.6
4,138,930	+	ptsA	b3947	0.47	-0.1
4,139,012	-	ptsA	b3947	0.51	+0.7
4,139,032	-	ptsA	b3947	0.52	-0.1
4,139,032	-	ptsA	b3947	0.52	+0.0
4,139,070	+	ptsA	b3947	0.53	-2.6
4,139,070	+	ptsA	b3947	0.53	+1.7
4,139,078	-	ptsA	b3947	0.53	-0.0
4,139,128	-	ptsA	b3947	0.55	-0.7
4,139,148	+	ptsA	b3947	0.56	-0.2
4,139,152	+	ptsA	b3947	0.56	-1.1
4,139,152	+	ptsA	b3947	0.56	+0.3
4,139,249	+	ptsA	b3947	0.60	-0.9
4,139,249	+	ptsA	b3947	0.60	-0.5
4,139,303	-	ptsA	b3947	0.62	-0.8
4,139,422	+	ptsA	b3947	0.67	-1.9
4,139,438	-	ptsA	b3947	0.68	-0.0
4,139,709	-	ptsA	b3947	0.79	-1.6
4,139,770	-	ptsA	b3947	0.81	-0.4
4,139,782	-	ptsA	b3947	0.81	-1.9
4,139,782	-	ptsA	b3947	0.81	+0.5
4,139,787	-	ptsA	b3947	0.82	+0.5
4,139,878	+	ptsA	b3947	0.85	-3.3
4,139,888	-	ptsA	b3947	0.86	+0.7
4,139,891	+	ptsA	b3947	0.86	+0.0
4,140,001	+				-0.5
4,140,001	+				+1.3
4,140,009	+				-1.4
4,140,096	-				+0.5
4,140,130	+				+0.2
4,140,133	-				+0.5
4,140,133	-				+0.9
4,140,196	+				-2.8
4,140,196	+				+0.8
4,140,204	-				+0.5
4,140,206	+				-2.6
4,140,206	+				+1.7
4,140,285	-				+0.9
4,140,295	+				-0.3
4,140,539	+				-1.1
4,140,667	+	frwC	b3949	0.11	+1.0
4,140,667	+	frwC	b3949	0.11	+0.8
4,140,685	+	frwC	b3949	0.12	+0.4
4,140,685	+	frwC	b3949	0.12	-0.5
4,140,685	+	frwC	b3949	0.12	+1.9
4,140,693	-	frwC	b3949	0.13	-3.0
4,140,756	+	frwC	b3949	0.19	-1.0
4,140,805	+	frwC	b3949	0.23	-1.1
4,140,808	-	frwC	b3949	0.24	+1.0
4,140,827	+	frwC	b3949	0.25	+0.7
4,140,827	+	frwC	b3949	0.25	-0.5
4,140,830	+	frwC	b3949	0.26	+0.5
4,140,895	+	frwC	b3949	0.32	+2.1
4,140,895	+	frwC	b3949	0.32	+1.4
4,140,951	+	frwC	b3949	0.37	+0.4
4,141,002	-	frwC	b3949	0.42	+0.7
4,141,010	+	frwC	b3949	0.42	-0.2
4,141,010	+	frwC	b3949	0.42	+0.3
4,141,051	-	frwC	b3949	0.46	-3.5
4,141,066	+	frwC	b3949	0.47	-0.1
4,141,069	+	frwC	b3949	0.48	-2.8
4,141,069	+	frwC	b3949	0.48	+2.1
4,141,069	+	frwC	b3949	0.48	+1.6
4,141,092	-	frwC	b3949	0.50	+1.0
4,141,105	+	frwC	b3949	0.51	+0.8
4,141,130	-	frwC	b3949	0.53	-0.6
4,141,133	+	frwC	b3949	0.54	+0.6
4,141,154	+	frwC	b3949	0.56	-1.0
4,141,167	+	frwC	b3949	0.57	+0.5
4,141,191	-	frwC	b3949	0.59	-0.5
4,141,194	-	frwC	b3949	0.59	+1.3
4,141,194	-	frwC	b3949	0.59	-3.4
4,141,218	+	frwC	b3949	0.62	-3.2
4,141,218	+	frwC	b3949	0.62	-0.6