Strain Fitness in Escherichia coli BW25113 around b3820
Experiment: T7_phage 0.00015625 MOI TC3
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | T7_phage 0.00015625 MOI TC3 |
---|---|---|---|---|---|
remove | |||||
4,001,253 | + | +3.0 | |||
4,001,262 | - | -3.7 | |||
4,001,262 | - | -0.4 | |||
4,001,263 | - | +1.7 | |||
4,001,263 | - | -0.7 | |||
4,001,263 | - | -0.0 | |||
4,001,268 | + | +3.0 | |||
4,001,274 | - | +1.4 | |||
4,001,276 | - | +1.1 | |||
4,001,276 | - | +3.0 | |||
4,001,276 | - | -0.5 | |||
4,001,276 | - | -2.4 | |||
4,001,276 | - | +0.4 | |||
4,001,276 | - | -1.3 | |||
4,001,277 | - | +2.2 | |||
4,001,277 | - | +0.2 | |||
4,001,279 | - | +2.5 | |||
4,001,329 | - | +0.9 | |||
4,001,329 | - | +0.9 | |||
4,001,329 | - | +0.6 | |||
4,001,329 | - | +2.0 | |||
4,001,329 | - | -2.9 | |||
4,001,329 | - | +0.4 | |||
4,001,329 | - | +0.9 | |||
4,001,329 | - | +0.3 | |||
4,001,329 | - | +1.2 | |||
4,001,329 | - | -0.1 | |||
4,001,329 | - | +1.7 | |||
4,001,329 | - | -0.6 | |||
4,001,329 | - | -1.8 | |||
4,001,329 | - | +1.4 | |||
4,001,329 | - | -2.7 | |||
4,001,329 | - | -2.4 | |||
4,001,329 | - | +0.9 | |||
4,001,329 | - | +0.6 | |||
4,001,329 | - | +1.7 | |||
4,001,329 | - | +0.6 | |||
4,001,329 | - | -0.9 | |||
4,001,329 | - | +2.3 | |||
4,001,331 | - | +2.1 | |||
4,001,331 | - | -1.0 | |||
4,001,338 | - | -0.5 | |||
4,001,338 | - | +2.4 | |||
4,001,339 | - | -0.2 | |||
4,001,339 | - | -0.8 | |||
4,001,339 | - | +0.9 | |||
4,001,341 | - | +0.3 | |||
4,001,341 | - | -0.1 | |||
4,001,347 | + | +3.0 | |||
4,001,347 | + | +3.0 | |||
4,001,372 | - | +1.1 | |||
4,001,386 | - | -2.6 | |||
4,001,386 | - | +1.0 | |||
4,001,387 | + | +2.0 | |||
4,001,395 | + | +1.4 | |||
4,001,395 | - | -0.4 | |||
4,001,403 | - | rarD | b3819 | 0.10 | +0.6 |
4,001,417 | - | rarD | b3819 | 0.12 | +1.3 |
4,001,448 | - | rarD | b3819 | 0.15 | -1.9 |
4,001,453 | + | rarD | b3819 | 0.16 | -0.0 |
4,001,453 | + | rarD | b3819 | 0.16 | -0.6 |
4,001,461 | - | rarD | b3819 | 0.17 | +1.5 |
4,001,461 | - | rarD | b3819 | 0.17 | -3.1 |
4,001,467 | - | rarD | b3819 | 0.18 | -3.8 |
4,001,467 | - | rarD | b3819 | 0.18 | +0.9 |
4,001,467 | - | rarD | b3819 | 0.18 | -0.0 |
4,001,469 | - | rarD | b3819 | 0.18 | -0.7 |
4,001,474 | - | rarD | b3819 | 0.18 | +0.7 |
4,001,478 | + | rarD | b3819 | 0.19 | +3.0 |
4,001,485 | - | rarD | b3819 | 0.20 | -0.3 |
4,001,486 | - | rarD | b3819 | 0.20 | -0.2 |
4,001,486 | - | rarD | b3819 | 0.20 | -3.6 |
4,001,530 | - | rarD | b3819 | 0.25 | +1.5 |
4,001,530 | - | rarD | b3819 | 0.25 | +0.1 |
4,001,533 | + | rarD | b3819 | 0.25 | +0.9 |
4,001,533 | + | rarD | b3819 | 0.25 | +1.2 |
4,001,586 | + | rarD | b3819 | 0.31 | +0.2 |
4,001,597 | + | rarD | b3819 | 0.32 | +1.4 |
4,001,597 | + | rarD | b3819 | 0.32 | +0.4 |
4,001,597 | + | rarD | b3819 | 0.32 | -1.4 |
4,001,605 | + | rarD | b3819 | 0.33 | -0.5 |
4,001,605 | - | rarD | b3819 | 0.33 | -0.2 |
4,001,613 | - | rarD | b3819 | 0.34 | -0.8 |
4,001,615 | - | rarD | b3819 | 0.34 | +0.7 |
4,001,615 | - | rarD | b3819 | 0.34 | -3.1 |
4,001,650 | + | rarD | b3819 | 0.38 | -0.3 |
4,001,653 | + | rarD | b3819 | 0.38 | +0.7 |
4,001,654 | - | rarD | b3819 | 0.38 | +0.8 |
4,001,662 | - | rarD | b3819 | 0.39 | -0.1 |
4,001,687 | - | rarD | b3819 | 0.42 | -2.3 |
4,001,687 | - | rarD | b3819 | 0.42 | -0.4 |
4,001,695 | + | rarD | b3819 | 0.43 | +1.4 |
4,001,698 | - | rarD | b3819 | 0.43 | -0.4 |
4,001,700 | + | rarD | b3819 | 0.44 | +0.2 |
4,001,700 | + | rarD | b3819 | 0.44 | +1.0 |
4,001,700 | - | rarD | b3819 | 0.44 | +0.9 |
4,001,701 | - | rarD | b3819 | 0.44 | -2.8 |
4,001,703 | - | rarD | b3819 | 0.44 | +0.3 |
4,001,708 | - | rarD | b3819 | 0.45 | -3.4 |
4,001,709 | - | rarD | b3819 | 0.45 | +1.5 |
4,001,709 | - | rarD | b3819 | 0.45 | +1.0 |
4,001,711 | - | rarD | b3819 | 0.45 | +0.4 |
4,001,771 | + | rarD | b3819 | 0.52 | -1.0 |
4,001,779 | - | rarD | b3819 | 0.53 | -0.9 |
4,001,779 | - | rarD | b3819 | 0.53 | +1.5 |
4,001,780 | + | rarD | b3819 | 0.53 | +1.7 |
4,001,781 | + | rarD | b3819 | 0.53 | +0.9 |
4,001,783 | - | rarD | b3819 | 0.53 | -1.4 |
4,001,830 | - | rarD | b3819 | 0.58 | -0.5 |
4,001,839 | + | rarD | b3819 | 0.59 | +1.3 |
4,001,851 | + | rarD | b3819 | 0.61 | +0.6 |
4,001,859 | - | rarD | b3819 | 0.62 | +0.9 |
4,001,898 | + | rarD | b3819 | 0.66 | +0.9 |
4,001,898 | + | rarD | b3819 | 0.66 | +1.3 |
4,001,899 | + | rarD | b3819 | 0.66 | -0.0 |
4,001,917 | + | rarD | b3819 | 0.68 | -0.7 |
4,001,917 | - | rarD | b3819 | 0.68 | -2.8 |
4,001,917 | - | rarD | b3819 | 0.68 | +1.3 |
4,001,920 | - | rarD | b3819 | 0.68 | -0.3 |
4,001,929 | + | rarD | b3819 | 0.69 | -2.3 |
4,001,929 | + | rarD | b3819 | 0.69 | -2.8 |
4,001,929 | + | rarD | b3819 | 0.69 | -3.1 |
4,001,929 | + | rarD | b3819 | 0.69 | -2.8 |
4,001,929 | + | rarD | b3819 | 0.69 | -0.2 |
4,001,929 | + | rarD | b3819 | 0.69 | -0.4 |
4,001,929 | + | rarD | b3819 | 0.69 | +0.5 |
4,001,937 | - | rarD | b3819 | 0.70 | +1.4 |
4,001,937 | - | rarD | b3819 | 0.70 | -0.8 |
4,001,937 | - | rarD | b3819 | 0.70 | +0.4 |
4,001,939 | + | rarD | b3819 | 0.70 | +0.4 |
4,001,950 | + | rarD | b3819 | 0.72 | +0.4 |
4,001,953 | + | rarD | b3819 | 0.72 | -1.7 |
4,001,953 | + | +2.4 | |||
4,001,953 | + | -0.0 | |||
4,001,956 | - | rarD | b3819 | 0.72 | -0.5 |
4,001,956 | - | rarD | b3819 | 0.72 | +1.5 |
4,001,959 | - | rarD | b3819 | 0.73 | -1.1 |
4,001,961 | + | rarD | b3819 | 0.73 | -1.9 |
4,001,961 | - | rarD | b3819 | 0.73 | -0.5 |
4,001,961 | - | rarD | b3819 | 0.73 | +1.2 |
4,001,961 | - | rarD | b3819 | 0.73 | +0.2 |
4,001,961 | - | rarD | b3819 | 0.73 | -2.1 |
4,001,961 | - | rarD | b3819 | 0.73 | +1.6 |
4,001,961 | - | rarD | b3819 | 0.73 | -0.2 |
4,001,962 | + | rarD | b3819 | 0.73 | -1.0 |
4,001,962 | + | rarD | b3819 | 0.73 | -3.7 |
4,001,962 | + | rarD | b3819 | 0.73 | +0.8 |
4,001,963 | + | rarD | b3819 | 0.73 | +0.1 |
4,001,970 | - | rarD | b3819 | 0.74 | +1.2 |
4,002,004 | - | rarD | b3819 | 0.78 | +1.4 |
4,002,004 | - | rarD | b3819 | 0.78 | -1.5 |
4,002,009 | + | rarD | b3819 | 0.78 | +0.9 |
4,002,035 | + | rarD | b3819 | 0.81 | +0.1 |
4,002,035 | + | rarD | b3819 | 0.81 | -0.6 |
4,002,038 | - | rarD | b3819 | 0.82 | -0.3 |
4,002,040 | + | rarD | b3819 | 0.82 | +0.3 |
4,002,043 | + | rarD | b3819 | 0.82 | +0.7 |
4,002,050 | + | rarD | b3819 | 0.83 | +0.3 |
4,002,051 | + | rarD | b3819 | 0.83 | +0.4 |
4,002,051 | - | rarD | b3819 | 0.83 | +0.3 |
4,002,051 | - | rarD | b3819 | 0.83 | +0.8 |
4,002,051 | - | rarD | b3819 | 0.83 | -0.7 |
4,002,051 | - | rarD | b3819 | 0.83 | +0.6 |
4,002,051 | - | rarD | b3819 | 0.83 | -1.4 |
4,002,064 | + | rarD | b3819 | 0.85 | +1.5 |
4,002,064 | + | rarD | b3819 | 0.85 | +2.0 |
4,002,087 | - | rarD | b3819 | 0.87 | +0.9 |
4,002,115 | - | -0.7 | |||
4,002,115 | - | -2.3 | |||
4,002,118 | - | +0.6 | |||
4,002,175 | + | +0.9 | |||
4,002,176 | + | +0.4 | |||
4,002,199 | + | +0.3 | |||
4,002,199 | + | +1.7 | |||
4,002,231 | - | +0.7 | |||
4,002,256 | + | +0.8 | |||
4,002,258 | + | -1.1 | |||
4,002,270 | - | -0.1 | |||
4,002,283 | + | -2.4 | |||
4,002,283 | + | +0.8 | |||
4,002,295 | - | +1.8 | |||
4,002,295 | - | -0.7 | |||
4,002,312 | - | yigI | b3820 | 0.13 | -2.8 |
4,002,333 | + | yigI | b3820 | 0.17 | +0.2 |
4,002,354 | + | yigI | b3820 | 0.22 | -1.5 |
4,002,354 | + | yigI | b3820 | 0.22 | -0.0 |
4,002,354 | + | yigI | b3820 | 0.22 | +3.0 |
4,002,363 | - | yigI | b3820 | 0.24 | -3.4 |
4,002,363 | - | yigI | b3820 | 0.24 | -1.0 |
4,002,376 | - | yigI | b3820 | 0.26 | +0.8 |
4,002,380 | + | yigI | b3820 | 0.27 | -1.1 |
4,002,388 | - | yigI | b3820 | 0.29 | -0.1 |
4,002,388 | - | yigI | b3820 | 0.29 | +1.0 |
4,002,388 | - | yigI | b3820 | 0.29 | -0.8 |
4,002,388 | - | yigI | b3820 | 0.29 | +0.9 |
4,002,419 | + | yigI | b3820 | 0.35 | -0.1 |
4,002,425 | + | -2.9 | |||
4,002,430 | + | yigI | b3820 | 0.38 | -0.9 |
4,002,441 | + | yigI | b3820 | 0.40 | -1.7 |
4,002,441 | + | yigI | b3820 | 0.40 | -1.2 |
4,002,450 | + | yigI | b3820 | 0.42 | +1.1 |
4,002,450 | + | yigI | b3820 | 0.42 | +0.3 |
4,002,475 | + | yigI | b3820 | 0.47 | -0.9 |
4,002,483 | - | yigI | b3820 | 0.49 | +1.8 |
4,002,485 | + | yigI | b3820 | 0.50 | -1.1 |
4,002,509 | - | yigI | b3820 | 0.55 | +1.4 |
4,002,519 | - | yigI | b3820 | 0.57 | +0.7 |
4,002,526 | + | yigI | b3820 | 0.58 | -0.5 |
4,002,614 | + | yigI | b3820 | 0.77 | -0.6 |
4,002,622 | + | yigI | b3820 | 0.79 | +0.4 |
4,002,622 | + | yigI | b3820 | 0.79 | +0.9 |
4,002,622 | + | yigI | b3820 | 0.79 | -0.6 |
4,002,622 | + | yigI | b3820 | 0.79 | +0.6 |
4,002,627 | + | yigI | b3820 | 0.80 | +1.2 |
4,002,627 | + | yigI | b3820 | 0.80 | -3.2 |
4,002,630 | - | yigI | b3820 | 0.81 | -3.3 |
4,002,660 | + | yigI | b3820 | 0.87 | -3.3 |
4,002,678 | - | +0.4 | |||
4,002,685 | + | -0.1 | |||
4,002,693 | - | -0.0 | |||
4,002,708 | + | -0.6 | |||
4,002,708 | + | +0.8 | |||
4,002,708 | + | +1.0 | |||
4,002,725 | + | +0.9 | |||
4,002,725 | + | -0.5 | |||
4,002,725 | + | +1.5 | |||
4,002,800 | + | -3.7 | |||
4,002,803 | - | -3.0 | |||
4,002,808 | - | -2.2 | |||
4,002,808 | - | +0.4 | |||
4,002,813 | + | -2.0 | |||
4,002,874 | + | -2.4 | |||
4,002,884 | + | -0.2 | |||
4,002,893 | - | +2.0 | |||
4,002,893 | - | -2.6 | |||
4,002,894 | + | +0.2 | |||
4,002,894 | + | -0.2 | |||
4,002,900 | - | -0.7 | |||
4,002,908 | - | +1.4 | |||
4,002,915 | + | -2.3 | |||
4,002,915 | + | +1.3 | |||
4,002,924 | - | +0.3 | |||
4,002,924 | - | +0.2 | |||
4,002,940 | + | -1.9 | |||
4,002,946 | - | -1.7 | |||
4,002,957 | + | -0.9 | |||
4,002,981 | - | pldA | b3821 | 0.11 | -0.3 |
4,002,981 | - | pldA | b3821 | 0.11 | -0.2 |
4,002,984 | + | pldA | b3821 | 0.11 | -1.3 |
4,003,000 | - | pldA | b3821 | 0.13 | +0.6 |
4,003,000 | - | pldA | b3821 | 0.13 | +1.3 |
4,003,022 | + | pldA | b3821 | 0.16 | +1.0 |
4,003,022 | + | pldA | b3821 | 0.16 | +0.6 |
4,003,050 | - | pldA | b3821 | 0.19 | +0.3 |
4,003,076 | + | pldA | b3821 | 0.22 | +0.9 |
4,003,088 | + | pldA | b3821 | 0.23 | +0.6 |
4,003,250 | - | pldA | b3821 | 0.42 | +2.2 |
4,003,250 | - | pldA | b3821 | 0.42 | -1.1 |
4,003,259 | + | pldA | b3821 | 0.43 | +0.6 |
4,003,261 | + | pldA | b3821 | 0.43 | -0.1 |
4,003,348 | + | pldA | b3821 | 0.53 | -0.6 |
4,003,356 | - | pldA | b3821 | 0.54 | -3.2 |
4,003,359 | + | pldA | b3821 | 0.54 | -0.5 |
4,003,359 | + | pldA | b3821 | 0.54 | +1.8 |
4,003,367 | - | pldA | b3821 | 0.55 | +0.1 |
4,003,367 | - | pldA | b3821 | 0.55 | -0.7 |
4,003,367 | - | pldA | b3821 | 0.55 | +0.6 |
4,003,367 | - | pldA | b3821 | 0.55 | -0.7 |
4,003,389 | + | pldA | b3821 | 0.58 | +0.3 |
4,003,412 | - | pldA | b3821 | 0.61 | +1.0 |
4,003,415 | + | pldA | b3821 | 0.61 | +0.3 |
4,003,415 | + | pldA | b3821 | 0.61 | +0.9 |
4,003,472 | + | pldA | b3821 | 0.67 | -1.1 |
4,003,472 | + | pldA | b3821 | 0.67 | -0.3 |
4,003,476 | + | pldA | b3821 | 0.68 | -2.9 |
4,003,477 | - | pldA | b3821 | 0.68 | -2.7 |
4,003,477 | - | pldA | b3821 | 0.68 | -1.2 |
4,003,480 | - | pldA | b3821 | 0.68 | +1.1 |
4,003,484 | - | pldA | b3821 | 0.69 | -0.2 |
4,003,484 | - | pldA | b3821 | 0.69 | -0.4 |
4,003,489 | - | pldA | b3821 | 0.69 | +2.0 |
4,003,512 | + | pldA | b3821 | 0.72 | +0.6 |
4,003,512 | + | pldA | b3821 | 0.72 | -0.3 |
4,003,586 | - | pldA | b3821 | 0.81 | +0.1 |
4,003,614 | + | pldA | b3821 | 0.84 | +0.7 |
4,003,614 | + | pldA | b3821 | 0.84 | +0.6 |
4,003,616 | + | pldA | b3821 | 0.84 | +0.7 |
4,003,620 | + | pldA | b3821 | 0.84 | +0.4 |
4,003,632 | - | pldA | b3821 | 0.86 | +0.4 |
4,003,632 | - | pldA | b3821 | 0.86 | -0.8 |
4,003,660 | - | pldA | b3821 | 0.89 | -1.8 |
Or see this region's nucleotide sequence