Experiment: T7_phage 0.00015625 MOI TC3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yggM and ansB are separated by 116 nucleotides ansB and yggN are separated by 175 nucleotides
b2956: yggM - hypothetical protein (NCBI), at 3,096,580 to 3,097,587
yggM
b2957: ansB - periplasmic L-asparaginase II (NCBI), at 3,097,704 to 3,098,750
ansB
b2958: yggN - hypothetical protein (NCBI), at 3,098,926 to 3,099,645
yggN
Position (kb)
3097
3098
3099 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3096.846 kb on + strand, within yggM at 3096.847 kb on - strand, within yggM at 3096.858 kb on + strand, within yggM at 3096.866 kb on - strand, within yggM at 3096.866 kb on - strand, within yggM at 3096.899 kb on - strand, within yggM at 3097.084 kb on - strand, within yggM at 3097.107 kb on - strand, within yggM at 3097.207 kb on - strand, within yggM at 3097.252 kb on + strand, within yggM at 3097.252 kb on + strand, within yggM at 3097.420 kb on - strand, within yggM at 3097.420 kb on - strand, within yggM at 3097.534 kb on - strand at 3097.548 kb on + strand at 3097.549 kb on - strand at 3097.557 kb on + strand at 3097.560 kb on - strand at 3097.560 kb on - strand at 3097.567 kb on - strand at 3097.660 kb on + strand at 3097.695 kb on - strand at 3097.695 kb on - strand at 3097.702 kb on + strand at 3097.702 kb on + strand at 3097.710 kb on - strand at 3097.796 kb on + strand at 3097.951 kb on + strand, within ansB at 3097.958 kb on - strand, within ansB at 3098.021 kb on - strand, within ansB at 3098.063 kb on + strand, within ansB at 3098.114 kb on + strand, within ansB at 3098.133 kb on - strand, within ansB at 3098.157 kb on + strand, within ansB at 3098.157 kb on + strand, within ansB at 3098.275 kb on - strand, within ansB at 3098.275 kb on - strand, within ansB at 3098.288 kb on - strand, within ansB at 3098.288 kb on - strand, within ansB at 3098.411 kb on - strand, within ansB at 3098.411 kb on - strand, within ansB at 3098.420 kb on - strand, within ansB at 3098.429 kb on - strand, within ansB at 3098.493 kb on - strand, within ansB at 3098.500 kb on + strand, within ansB at 3098.500 kb on + strand, within ansB at 3098.536 kb on + strand, within ansB at 3098.631 kb on + strand, within ansB at 3098.631 kb on - strand, within ansB at 3098.631 kb on - strand, within ansB at 3098.647 kb on - strand at 3098.647 kb on - strand at 3098.684 kb on + strand at 3098.706 kb on - strand at 3098.706 kb on - strand at 3099.052 kb on + strand, within yggN at 3099.062 kb on + strand, within yggN at 3099.190 kb on + strand, within yggN at 3099.216 kb on + strand, within yggN at 3099.216 kb on + strand, within yggN at 3099.347 kb on + strand, within yggN at 3099.350 kb on - strand, within yggN at 3099.350 kb on - strand, within yggN at 3099.366 kb on + strand, within yggN at 3099.366 kb on + strand, within yggN at 3099.482 kb on - strand, within yggN at 3099.484 kb on - strand, within yggN at 3099.533 kb on + strand, within yggN at 3099.564 kb on + strand, within yggN at 3099.564 kb on + strand, within yggN at 3099.564 kb on + strand, within yggN at 3099.569 kb on + strand, within yggN
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC3 remove 3,096,846 + yggM b2956 0.26 -3.0 3,096,847 - yggM b2956 0.26 -1.3 3,096,858 + yggM b2956 0.28 -0.1 3,096,866 - yggM b2956 0.28 +1.3 3,096,866 - yggM b2956 0.28 +1.4 3,096,899 - yggM b2956 0.32 -1.3 3,097,084 - yggM b2956 0.50 -2.8 3,097,107 - yggM b2956 0.52 -1.1 3,097,207 - yggM b2956 0.62 -0.9 3,097,252 + yggM b2956 0.67 +0.1 3,097,252 + yggM b2956 0.67 -2.7 3,097,420 - yggM b2956 0.83 +0.2 3,097,420 - yggM b2956 0.83 +0.9 3,097,534 - -1.3 3,097,548 + -2.6 3,097,549 - +3.7 3,097,557 + -2.7 3,097,560 - +1.4 3,097,560 - -2.1 3,097,567 - -3.5 3,097,660 + -0.4 3,097,695 - -3.7 3,097,695 - +0.3 3,097,702 + -0.4 3,097,702 + -0.6 3,097,710 - -2.4 3,097,796 + -0.9 3,097,951 + ansB b2957 0.24 -1.7 3,097,958 - ansB b2957 0.24 +0.2 3,098,021 - ansB b2957 0.30 -3.0 3,098,063 + ansB b2957 0.34 -0.9 3,098,114 + ansB b2957 0.39 +0.5 3,098,133 - ansB b2957 0.41 +0.8 3,098,157 + ansB b2957 0.43 +1.1 3,098,157 + ansB b2957 0.43 -2.1 3,098,275 - ansB b2957 0.55 -2.5 3,098,275 - ansB b2957 0.55 -0.3 3,098,288 - ansB b2957 0.56 -0.3 3,098,288 - ansB b2957 0.56 +0.2 3,098,411 - ansB b2957 0.68 -3.2 3,098,411 - ansB b2957 0.68 +2.5 3,098,420 - ansB b2957 0.68 -0.6 3,098,429 - ansB b2957 0.69 +1.1 3,098,493 - ansB b2957 0.75 -0.1 3,098,500 + ansB b2957 0.76 -0.1 3,098,500 + ansB b2957 0.76 +0.2 3,098,536 + ansB b2957 0.79 -1.3 3,098,631 + ansB b2957 0.89 +0.9 3,098,631 - ansB b2957 0.89 -0.1 3,098,631 - ansB b2957 0.89 -4.4 3,098,647 - -1.8 3,098,647 - +1.7 3,098,684 + +1.0 3,098,706 - -0.8 3,098,706 - +0.2 3,099,052 + yggN b2958 0.17 -1.9 3,099,062 + yggN b2958 0.19 +0.7 3,099,190 + yggN b2958 0.37 -2.8 3,099,216 + yggN b2958 0.40 -3.0 3,099,216 + yggN b2958 0.40 -1.6 3,099,347 + yggN b2958 0.58 -3.1 3,099,350 - yggN b2958 0.59 +1.3 3,099,350 - yggN b2958 0.59 -1.6 3,099,366 + yggN b2958 0.61 -2.3 3,099,366 + yggN b2958 0.61 -1.7 3,099,482 - yggN b2958 0.77 -1.6 3,099,484 - yggN b2958 0.78 -0.1 3,099,533 + yggN b2958 0.84 -2.4 3,099,564 + yggN b2958 0.89 -3.5 3,099,564 + yggN b2958 0.89 +0.9 3,099,564 + yggN b2958 0.89 -1.1 3,099,569 + yggN b2958 0.89 -2.0
Or see this region's nucleotide sequence