Experiment: T7_phage 0.00015625 MOI TC3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bglA and ygfF are separated by 56 nucleotides ygfF and gcvP are separated by 266 nucleotides
b2901: bglA - 6-phospho-beta-glucosidase A (NCBI), at 3,041,684 to 3,043,123
bglA
b2902: ygfF - predicted NAD(P)-binding oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 3,043,180 to 3,043,923
ygfF
b2903: gcvP - glycine dehydrogenase (NCBI), at 3,044,190 to 3,047,063
gcvP
Position (kb)
3043
3044 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3042.251 kb on - strand, within bglA at 3042.251 kb on - strand, within bglA at 3042.253 kb on + strand, within bglA at 3042.280 kb on + strand, within bglA at 3042.280 kb on + strand, within bglA at 3042.280 kb on + strand, within bglA at 3042.288 kb on - strand, within bglA at 3042.288 kb on - strand, within bglA at 3042.288 kb on - strand, within bglA at 3042.288 kb on - strand, within bglA at 3042.288 kb on - strand, within bglA at 3042.410 kb on - strand, within bglA at 3042.429 kb on - strand, within bglA at 3042.434 kb on - strand, within bglA at 3042.434 kb on - strand, within bglA at 3042.466 kb on + strand, within bglA at 3042.466 kb on + strand, within bglA at 3042.524 kb on - strand, within bglA at 3042.524 kb on - strand, within bglA at 3042.531 kb on + strand, within bglA at 3042.586 kb on + strand, within bglA at 3042.603 kb on - strand, within bglA at 3042.707 kb on - strand, within bglA at 3042.720 kb on - strand, within bglA at 3042.760 kb on - strand, within bglA at 3042.761 kb on - strand, within bglA at 3042.784 kb on - strand, within bglA at 3042.784 kb on - strand, within bglA at 3042.793 kb on - strand, within bglA at 3042.878 kb on + strand, within bglA at 3043.041 kb on + strand at 3043.041 kb on + strand at 3043.049 kb on - strand at 3043.049 kb on - strand at 3043.088 kb on + strand at 3043.088 kb on + strand at 3043.148 kb on - strand at 3043.148 kb on - strand at 3043.148 kb on - strand at 3043.148 kb on - strand at 3043.185 kb on - strand at 3043.248 kb on - strand at 3043.275 kb on + strand, within ygfF at 3043.295 kb on - strand, within ygfF at 3043.423 kb on + strand, within ygfF at 3043.423 kb on + strand, within ygfF at 3043.610 kb on - strand, within ygfF at 3043.610 kb on - strand, within ygfF at 3043.632 kb on + strand, within ygfF at 3043.638 kb on - strand, within ygfF at 3043.658 kb on + strand, within ygfF at 3043.809 kb on - strand, within ygfF at 3043.836 kb on - strand, within ygfF at 3043.903 kb on + strand at 3043.903 kb on + strand at 3043.911 kb on - strand at 3043.911 kb on - strand at 3044.077 kb on - strand at 3044.226 kb on - strand at 3044.282 kb on - strand at 3044.282 kb on - strand at 3044.308 kb on + strand at 3044.317 kb on - strand at 3044.317 kb on - strand at 3044.650 kb on + strand, within gcvP at 3044.777 kb on + strand, within gcvP at 3044.785 kb on - strand, within gcvP at 3044.785 kb on - strand, within gcvP at 3044.785 kb on - strand, within gcvP at 3044.788 kb on + strand, within gcvP at 3044.788 kb on + strand, within gcvP at 3044.798 kb on - strand, within gcvP at 3044.831 kb on - strand, within gcvP
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC3 remove 3,042,251 - bglA b2901 0.39 -3.5 3,042,251 - bglA b2901 0.39 -0.4 3,042,253 + bglA b2901 0.40 -2.0 3,042,280 + bglA b2901 0.41 +2.0 3,042,280 + bglA b2901 0.41 -2.3 3,042,280 + bglA b2901 0.41 +0.7 3,042,288 - bglA b2901 0.42 +1.5 3,042,288 - bglA b2901 0.42 -2.5 3,042,288 - bglA b2901 0.42 +0.7 3,042,288 - bglA b2901 0.42 +1.6 3,042,288 - bglA b2901 0.42 +0.4 3,042,410 - bglA b2901 0.50 -2.0 3,042,429 - bglA b2901 0.52 +0.2 3,042,434 - bglA b2901 0.52 +0.4 3,042,434 - bglA b2901 0.52 -2.7 3,042,466 + bglA b2901 0.54 -0.2 3,042,466 + bglA b2901 0.54 -1.0 3,042,524 - bglA b2901 0.58 -2.5 3,042,524 - bglA b2901 0.58 -2.0 3,042,531 + bglA b2901 0.59 +2.0 3,042,586 + bglA b2901 0.63 -0.2 3,042,603 - bglA b2901 0.64 +0.4 3,042,707 - bglA b2901 0.71 +1.5 3,042,720 - bglA b2901 0.72 -1.9 3,042,760 - bglA b2901 0.75 -2.7 3,042,761 - bglA b2901 0.75 +1.7 3,042,784 - bglA b2901 0.76 -2.6 3,042,784 - bglA b2901 0.76 -0.5 3,042,793 - bglA b2901 0.77 -0.1 3,042,878 + bglA b2901 0.83 +0.2 3,043,041 + +1.7 3,043,041 + +0.3 3,043,049 - +0.9 3,043,049 - -0.1 3,043,088 + +0.6 3,043,088 + +1.1 3,043,148 - -2.0 3,043,148 - +1.5 3,043,148 - -3.6 3,043,148 - +0.6 3,043,185 - +0.2 3,043,248 - +0.3 3,043,275 + ygfF b2902 0.13 +1.7 3,043,295 - ygfF b2902 0.15 -4.5 3,043,423 + ygfF b2902 0.33 -0.1 3,043,423 + ygfF b2902 0.33 -3.0 3,043,610 - ygfF b2902 0.58 +0.8 3,043,610 - ygfF b2902 0.58 +0.5 3,043,632 + ygfF b2902 0.61 +0.3 3,043,638 - ygfF b2902 0.62 +1.3 3,043,658 + ygfF b2902 0.64 +0.5 3,043,809 - ygfF b2902 0.85 -0.1 3,043,836 - ygfF b2902 0.88 +2.7 3,043,903 + -3.5 3,043,903 + -0.3 3,043,911 - +0.9 3,043,911 - -2.0 3,044,077 - -0.0 3,044,226 - +0.3 3,044,282 - +0.8 3,044,282 - +1.2 3,044,308 + +0.9 3,044,317 - +2.1 3,044,317 - +2.2 3,044,650 + gcvP b2903 0.16 +1.4 3,044,777 + gcvP b2903 0.20 +2.1 3,044,785 - gcvP b2903 0.21 +1.9 3,044,785 - gcvP b2903 0.21 +2.5 3,044,785 - gcvP b2903 0.21 +1.1 3,044,788 + gcvP b2903 0.21 +2.3 3,044,788 + gcvP b2903 0.21 +1.2 3,044,798 - gcvP b2903 0.21 +0.9 3,044,831 - gcvP b2903 0.22 +1.7
Or see this region's nucleotide sequence