Strain Fitness in Escherichia coli BW25113 around b2119

Experiment: T7_phage 0.00015625 MOI TC3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyehI and yehK are separated by 60 nucleotidesyehK and yehL are separated by 306 nucleotidesyehL and yehM are separated by 10 nucleotides b2118: yehI - hypothetical protein (NCBI), at 2,198,301 to 2,201,933 yehI b4541: yehK - hypothetical protein (NCBI), at 2,201,994 to 2,202,311 yehK b2119: yehL - orf, hypothetical protein (VIMSS), at 2,202,618 to 2,203,706 yehL b2120: yehM - hypothetical protein (NCBI), at 2,203,717 to 2,205,996 yehM Position (kb) 2202 2203 2204Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 2201.645 kb on - strandat 2201.709 kb on + strandat 2201.709 kb on + strandat 2201.746 kb on + strandat 2201.764 kb on - strandat 2201.833 kb on + strandat 2201.833 kb on + strandat 2201.838 kb on + strandat 2201.955 kb on - strandat 2202.021 kb on + strandat 2202.040 kb on + strand, within yehKat 2202.102 kb on + strand, within yehKat 2202.114 kb on + strand, within yehKat 2202.116 kb on + strand, within yehKat 2202.125 kb on + strand, within yehKat 2202.125 kb on + strand, within yehKat 2202.135 kb on + strand, within yehKat 2202.152 kb on + strand, within yehKat 2202.166 kb on + strand, within yehKat 2202.166 kb on + strand, within yehKat 2202.166 kb on + strand, within yehKat 2202.174 kb on - strand, within yehKat 2202.199 kb on + strand, within yehKat 2202.431 kb on + strandat 2202.431 kb on + strandat 2202.437 kb on + strandat 2202.445 kb on + strandat 2202.445 kb on + strandat 2202.655 kb on + strandat 2202.662 kb on + strandat 2202.662 kb on + strandat 2202.663 kb on - strandat 2202.663 kb on - strandat 2202.663 kb on - strandat 2202.663 kb on - strandat 2202.665 kb on - strandat 2202.665 kb on - strandat 2202.665 kb on - strandat 2202.668 kb on - strandat 2202.670 kb on - strandat 2202.675 kb on - strandat 2202.675 kb on - strandat 2202.714 kb on - strandat 2202.719 kb on - strandat 2202.733 kb on - strand, within yehLat 2202.733 kb on - strand, within yehLat 2202.751 kb on + strand, within yehLat 2202.751 kb on + strand, within yehLat 2202.790 kb on + strand, within yehLat 2202.790 kb on + strand, within yehLat 2202.840 kb on - strand, within yehLat 2202.843 kb on - strand, within yehLat 2202.951 kb on + strand, within yehLat 2202.951 kb on + strand, within yehLat 2202.994 kb on - strand, within yehLat 2202.994 kb on - strand, within yehLat 2203.056 kb on - strand, within yehLat 2203.110 kb on + strand, within yehLat 2203.120 kb on + strand, within yehLat 2203.372 kb on + strand, within yehLat 2203.392 kb on + strand, within yehLat 2203.392 kb on + strand, within yehLat 2203.456 kb on + strand, within yehLat 2203.473 kb on + strand, within yehLat 2203.473 kb on + strand, within yehLat 2203.508 kb on + strand, within yehLat 2203.508 kb on + strand, within yehLat 2203.511 kb on - strand, within yehLat 2203.548 kb on + strand, within yehLat 2203.563 kb on - strand, within yehLat 2203.693 kb on + strandat 2203.903 kb on + strandat 2203.903 kb on + strandat 2203.918 kb on + strandat 2204.037 kb on + strand, within yehMat 2204.045 kb on - strand, within yehMat 2204.166 kb on + strandat 2204.172 kb on - strand, within yehMat 2204.172 kb on - strand, within yehMat 2204.208 kb on - strand, within yehMat 2204.213 kb on + strand, within yehMat 2204.313 kb on - strand, within yehMat 2204.313 kb on - strand, within yehMat 2204.399 kb on + strand, within yehMat 2204.639 kb on + strand, within yehMat 2204.639 kb on + strand, within yehMat 2204.639 kb on - strand, within yehMat 2204.670 kb on - strand, within yehM

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC3
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2,201,645 - +2.5
2,201,709 + +0.7
2,201,709 + +0.5
2,201,746 + +0.2
2,201,764 - +1.6
2,201,833 + -0.1
2,201,833 + -0.3
2,201,838 + +1.6
2,201,955 - -0.7
2,202,021 + +1.2
2,202,040 + yehK b4541 0.14 +3.9
2,202,102 + yehK b4541 0.34 +1.9
2,202,114 + yehK b4541 0.38 -0.4
2,202,116 + yehK b4541 0.38 -0.1
2,202,125 + yehK b4541 0.41 -0.3
2,202,125 + yehK b4541 0.41 +0.1
2,202,135 + yehK b4541 0.44 -0.4
2,202,152 + yehK b4541 0.50 +0.7
2,202,166 + yehK b4541 0.54 +1.1
2,202,166 + yehK b4541 0.54 -1.7
2,202,166 + yehK b4541 0.54 -0.7
2,202,174 - yehK b4541 0.57 +2.1
2,202,199 + yehK b4541 0.64 +2.1
2,202,431 + +2.1
2,202,431 + -1.2
2,202,437 + -1.9
2,202,445 + -0.2
2,202,445 + +1.1
2,202,655 + +2.1
2,202,662 + +2.1
2,202,662 + +2.1
2,202,663 - +0.1
2,202,663 - -0.0
2,202,663 - +0.9
2,202,663 - -0.1
2,202,665 - -1.2
2,202,665 - -0.3
2,202,665 - -2.2
2,202,668 - -2.9
2,202,670 - -2.7
2,202,675 - -1.6
2,202,675 - -1.2
2,202,714 - -2.4
2,202,719 - +0.6
2,202,733 - yehL b2119 0.11 -2.1
2,202,733 - yehL b2119 0.11 +0.1
2,202,751 + yehL b2119 0.12 +2.1
2,202,751 + yehL b2119 0.12 +2.1
2,202,790 + yehL b2119 0.16 +2.1
2,202,790 + yehL b2119 0.16 +3.3
2,202,840 - yehL b2119 0.20 +0.9
2,202,843 - yehL b2119 0.21 +0.5
2,202,951 + yehL b2119 0.31 +2.1
2,202,951 + yehL b2119 0.31 +1.1
2,202,994 - yehL b2119 0.35 -3.8
2,202,994 - yehL b2119 0.35 -0.4
2,203,056 - yehL b2119 0.40 +0.3
2,203,110 + yehL b2119 0.45 -3.9
2,203,120 + yehL b2119 0.46 -1.2
2,203,372 + yehL b2119 0.69 -0.9
2,203,392 + yehL b2119 0.71 -2.8
2,203,392 + yehL b2119 0.71 -1.1
2,203,456 + yehL b2119 0.77 -2.4
2,203,473 + yehL b2119 0.79 -2.1
2,203,473 + yehL b2119 0.79 -2.4
2,203,508 + yehL b2119 0.82 -1.2
2,203,508 + yehL b2119 0.82 -0.2
2,203,511 - yehL b2119 0.82 -1.4
2,203,548 + yehL b2119 0.85 +1.8
2,203,563 - yehL b2119 0.87 -0.7
2,203,693 + +1.8
2,203,903 + -3.3
2,203,903 + +0.1
2,203,918 + -2.9
2,204,037 + yehM b2120 0.14 +1.9
2,204,045 - yehM b2120 0.14 +1.0
2,204,166 + +0.2
2,204,172 - yehM b2120 0.20 +2.7
2,204,172 - yehM b2120 0.20 -0.4
2,204,208 - yehM b2120 0.22 -0.0
2,204,213 + yehM b2120 0.22 -3.4
2,204,313 - yehM b2120 0.26 +2.8
2,204,313 - yehM b2120 0.26 +0.6
2,204,399 + yehM b2120 0.30 +1.4
2,204,639 + yehM b2120 0.40 -1.1
2,204,639 + yehM b2120 0.40 -2.0
2,204,639 - yehM b2120 0.40 -1.6
2,204,670 - yehM b2120 0.42 +0.1

Or see this region's nucleotide sequence