Strain Fitness in Escherichia coli BW25113 around b2011

Experiment: T7_phage 0.00015625 MOI TC3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdacD and sbcB are separated by 208 nucleotidessbcB and yeeD are separated by 42 nucleotidesyeeD and yeeE are separated by 13 nucleotides b2010: dacD - penicillin binding protein 6b (VIMSS), at 2,079,405 to 2,080,571 dacD b2011: sbcB - exonuclease I (NCBI), at 2,080,780 to 2,082,207 sbcB b2012: yeeD - hypothetical protein (NCBI), at 2,082,250 to 2,082,477 yeeD b2013: yeeE - predicted inner membrane protein (NCBI), at 2,082,491 to 2,083,549 yeeE Position (kb) 2080 2081 2082 2083Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 2079.784 kb on + strand, within dacDat 2079.833 kb on - strand, within dacDat 2079.833 kb on - strand, within dacDat 2079.857 kb on + strand, within dacDat 2079.857 kb on + strand, within dacDat 2079.966 kb on + strand, within dacDat 2080.001 kb on + strand, within dacDat 2080.041 kb on + strand, within dacDat 2080.420 kb on + strand, within dacDat 2080.420 kb on + strand, within dacDat 2080.685 kb on - strandat 2080.685 kb on - strandat 2080.691 kb on - strandat 2080.744 kb on + strandat 2080.777 kb on - strandat 2080.787 kb on - strandat 2080.857 kb on - strandat 2080.857 kb on - strandat 2080.867 kb on - strandat 2080.867 kb on - strandat 2080.982 kb on + strand, within sbcBat 2080.982 kb on + strand, within sbcBat 2080.990 kb on - strand, within sbcBat 2081.062 kb on + strand, within sbcBat 2081.062 kb on + strand, within sbcBat 2081.077 kb on - strand, within sbcBat 2081.151 kb on + strand, within sbcBat 2081.153 kb on + strand, within sbcBat 2081.218 kb on - strand, within sbcBat 2081.220 kb on + strand, within sbcBat 2081.238 kb on - strand, within sbcBat 2081.289 kb on - strand, within sbcBat 2081.289 kb on - strand, within sbcBat 2081.300 kb on + strand, within sbcBat 2081.307 kb on + strand, within sbcBat 2081.308 kb on - strand, within sbcBat 2081.308 kb on - strand, within sbcBat 2081.410 kb on - strand, within sbcBat 2081.411 kb on + strand, within sbcBat 2081.411 kb on + strand, within sbcBat 2081.513 kb on + strand, within sbcBat 2081.518 kb on - strand, within sbcBat 2081.625 kb on + strand, within sbcBat 2081.675 kb on + strand, within sbcBat 2081.679 kb on + strand, within sbcBat 2081.688 kb on - strand, within sbcBat 2081.701 kb on + strand, within sbcBat 2081.862 kb on - strand, within sbcBat 2081.862 kb on - strand, within sbcBat 2081.885 kb on - strand, within sbcBat 2082.093 kb on + strandat 2082.093 kb on + strandat 2082.168 kb on + strandat 2082.266 kb on + strandat 2082.266 kb on + strandat 2082.266 kb on + strandat 2082.299 kb on + strand, within yeeDat 2082.299 kb on + strand, within yeeDat 2082.429 kb on - strand, within yeeDat 2082.537 kb on - strandat 2082.537 kb on - strandat 2082.544 kb on - strandat 2082.544 kb on - strandat 2082.546 kb on + strandat 2082.674 kb on + strand, within yeeEat 2082.694 kb on - strand, within yeeEat 2082.775 kb on - strand, within yeeEat 2082.775 kb on - strand, within yeeEat 2082.851 kb on - strand, within yeeEat 2082.881 kb on + strand, within yeeEat 2082.897 kb on - strand, within yeeEat 2082.897 kb on - strand, within yeeEat 2082.915 kb on - strand, within yeeEat 2082.973 kb on + strand, within yeeEat 2082.973 kb on + strand, within yeeEat 2082.981 kb on - strand, within yeeEat 2083.040 kb on - strand, within yeeEat 2083.040 kb on - strand, within yeeEat 2083.126 kb on + strand, within yeeEat 2083.139 kb on + strand, within yeeEat 2083.183 kb on + strand, within yeeEat 2083.186 kb on - strand, within yeeEat 2083.186 kb on - strand, within yeeE

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC3
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2,079,784 + dacD b2010 0.32 +0.5
2,079,833 - dacD b2010 0.37 -0.1
2,079,833 - dacD b2010 0.37 +1.4
2,079,857 + dacD b2010 0.39 +0.9
2,079,857 + dacD b2010 0.39 -1.1
2,079,966 + dacD b2010 0.48 +1.4
2,080,001 + dacD b2010 0.51 +0.5
2,080,041 + dacD b2010 0.54 -0.7
2,080,420 + dacD b2010 0.87 -0.6
2,080,420 + dacD b2010 0.87 +1.3
2,080,685 - -2.8
2,080,685 - -0.2
2,080,691 - +3.1
2,080,744 + -0.4
2,080,777 - +1.4
2,080,787 - -0.5
2,080,857 - +3.2
2,080,857 - +2.9
2,080,867 - +2.4
2,080,867 - +3.4
2,080,982 + sbcB b2011 0.14 +4.0
2,080,982 + sbcB b2011 0.14 +2.2
2,080,990 - sbcB b2011 0.15 +4.7
2,081,062 + sbcB b2011 0.20 +2.9
2,081,062 + sbcB b2011 0.20 +4.0
2,081,077 - sbcB b2011 0.21 +3.8
2,081,151 + sbcB b2011 0.26 +2.7
2,081,153 + sbcB b2011 0.26 +3.7
2,081,218 - sbcB b2011 0.31 +1.9
2,081,220 + sbcB b2011 0.31 +3.3
2,081,238 - sbcB b2011 0.32 +4.1
2,081,289 - sbcB b2011 0.36 +3.4
2,081,289 - sbcB b2011 0.36 +3.6
2,081,300 + sbcB b2011 0.36 +3.2
2,081,307 + sbcB b2011 0.37 +3.2
2,081,308 - sbcB b2011 0.37 +2.9
2,081,308 - sbcB b2011 0.37 +3.0
2,081,410 - sbcB b2011 0.44 +2.7
2,081,411 + sbcB b2011 0.44 +4.5
2,081,411 + sbcB b2011 0.44 +1.8
2,081,513 + sbcB b2011 0.51 +1.6
2,081,518 - sbcB b2011 0.52 +1.5
2,081,625 + sbcB b2011 0.59 +3.3
2,081,675 + sbcB b2011 0.63 +2.2
2,081,679 + sbcB b2011 0.63 +3.8
2,081,688 - sbcB b2011 0.64 +3.4
2,081,701 + sbcB b2011 0.64 +4.1
2,081,862 - sbcB b2011 0.76 +3.6
2,081,862 - sbcB b2011 0.76 +3.9
2,081,885 - sbcB b2011 0.77 +2.1
2,082,093 + +3.0
2,082,093 + +3.0
2,082,168 + +1.7
2,082,266 + -1.4
2,082,266 + +0.6
2,082,266 + -3.3
2,082,299 + yeeD b2012 0.21 +0.7
2,082,299 + yeeD b2012 0.21 -0.5
2,082,429 - yeeD b2012 0.79 +0.2
2,082,537 - -3.2
2,082,537 - +1.1
2,082,544 - +0.9
2,082,544 - +1.1
2,082,546 + +0.2
2,082,674 + yeeE b2013 0.17 +0.6
2,082,694 - yeeE b2013 0.19 +0.8
2,082,775 - yeeE b2013 0.27 +1.1
2,082,775 - yeeE b2013 0.27 +1.5
2,082,851 - yeeE b2013 0.34 +1.1
2,082,881 + yeeE b2013 0.37 +0.3
2,082,897 - yeeE b2013 0.38 -3.2
2,082,897 - yeeE b2013 0.38 +0.5
2,082,915 - yeeE b2013 0.40 +0.6
2,082,973 + yeeE b2013 0.46 -1.4
2,082,973 + yeeE b2013 0.46 -1.8
2,082,981 - yeeE b2013 0.46 -4.4
2,083,040 - yeeE b2013 0.52 +1.0
2,083,040 - yeeE b2013 0.52 +0.1
2,083,126 + yeeE b2013 0.60 -2.5
2,083,139 + yeeE b2013 0.61 +1.0
2,083,183 + yeeE b2013 0.65 -2.6
2,083,186 - yeeE b2013 0.66 +3.0
2,083,186 - yeeE b2013 0.66 +1.3

Or see this region's nucleotide sequence